LRRK2 | LRRK2 Resource

This reagent and more can be found at our sister site MRC PPU Reagents & Services

Request Now

Plasmid

pGEX-LRRK2-Kin Dom-Kdead

Parent Plasmid

pGEX6P1

Expressed

GST-LRRK2-Kin Dom-Kdead

DU Number

DU6129

Genbank

XM_058513, LRRK2 Sugen

Tag

GST

Species

Human

Sequence of Insert

View Sequence

GGATCCGCCCCTGACTTGATTTTGGCTGACCTGCCTAGAAATATTATGTT
GAATAGTGATGAGTTGGAATTTGAACAAGCTCCAGAGTTTCTCCTAGGTG
ATGGCAGTTTTGGATCAGTTTACCGAGCAGCCTATGAAGGAGAAGAAGTG
GCTGTGAAGATTTTTAATAAACATACATCACTCAGGCTGTTAAGACAAGA
GCTTGTGGTGCTTTGCCACCTCCACCACCCCAGTTTGATATCTTTGCTGG
CAGCTGGGATTCGTCCCCGGATGTTGGTGATGGAGTTAGCCTCCAAGGGT
TCCTTGGATCGCCTGCTTCAGCAGGACAAAGCCAGCCTCACTAGAACCCT
ACAGCACAGGATCGCACTCCACGTAGCTGATGGTTTGAGATACCTCCACT
CAGCCATGATTATATACCGAGACCTGAAACCCCACAATGTGCTGCTTTTC
ACACTGTATCCCAATGCTGCCATCATCGCAAAGATTGCTGACTACGGCAT
TGCTCAGTACTGCTGTAGAATGGGGATAAAAACATCAGAGGGCACACCAG
GGTTTCGTGCACCTGAAGTTGCCAGAGGAAATGTCATTTATAACCAACAG
GCTGATGTTTATTCATTTGGTTTACTACTCTATGACATTTTGACAACTGG
AGGTAGAATAGTAGAGGGTTTGAAGTTTCCAAATGAGTTTGATGAATTAG
AAATACAAGGAAAATTACCTGATCCAGTTAAAGAATATGGTTGTGCCCCA
TGGCCTATGGTTGAAAGATTAATTAAACAGTGTTTGAAAGAAAATCCTCA
AGAAAGGCCTACTTCTGCCCAGGTCTTTGACATTTTGAATTCAGCTGAAT
TAGTCTGTCTGACGAGACGCATTTTATAGGATCC

Amino Acid Sequence

View Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSAPDLILADLPRNI
MLNSDELEFEQAPEFLLGDGSFGSVYRAAYEGEEVAVKIFNKHTSLRLLR
QELVVLCHLHHPSLISLLAAGIRPRMLVMELASKGSLDRLLQQDKASLTR
TLQHRIALHVADGLRYLHSAMIIYRDLKPHNVLLFTLYPNAAIIAKIAAY
GIAQYCCRMGIKTSEGTPGFRAPEVARGNVIYNQQADVYSFGLLLYDILT
TGGRIVEGLKFPNEFDELEIQGKLPDPVKEYGCAPWPMVERLIKQCLKEN
PQERPTSAQVFDILNSAELVCLTRRIL*

Vector Type

Bacterial

Antibiotic

Amp

Comments

N terminal GST for mammalian expression containing catalytic domain of LRRK2. Differs to genbank at N1827S K2064R. Mutations also in DKFZp451G151 EST.
All LRRK2 plasmids MUST be grown at 30 degrees C or less to prevent recombination. Contains SNP S1647T

100042 R 1000 1001 1047 19007

Download datasheet