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Long Name Textual
RAB8A, member RAS oncogene family
MBP-Rab8A [Du 16222]
100041 R 1000 1001 1047 19007
HeLa Flp-in Trex cells stably expressing GFP-empty, GFP-Rab8A, GFP-Rab8B and GFP-Rab13 were induced by Doxycycline (0.1μg/ml) for 24h. The indicated protein extracts were subjected to SDS-PAGE analysis before transferred to nitrocellulose membrane and immunoblotted with Anti-GFP antibody or anti-Rab8A antibodies (bleed 1-4) overnight. Binding of the primary antibody was detected using anti-sheep (1:10,000) and visualised with ECL. Note: Only detect overexpressed Rab8A, the 4th bleed may detect endogenous Rab8A.