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Plasmid
pET15b 6HIS SUMO PPM1H Linker deleted
Parent Plasmid
pET15b 6His SUMO
Expressed
6HIS SUMO PPM1H
DU Number
DU62835
Genbank
Q9ULR3.2
Tag
6His
SUMO
Species
Human
Sequence of Insert
View Sequence
GGATCCATGCTCACTCGAGTGAAATCTGCCGTGGCCAATTTCATGGGCGGCATCATGGCTGGCAGCTCAGGCTCCGAGCACGGCGGCGGCAGCTGCGGAGGCTCGGACCTGCCCCTGCGTTTCCCCTACGGGCGGCCAGAGTTCCTGGGGCTGTCTCAGGACGAGGTGGAGTGCAGCGCCGACCACATCGCCCGCCCCATCCTCATCCTCAAGGAGACTCGGCGGCTGCCCTGGGCCACTGGCTACGCAGAGGTTATCAATGCCGGGAAGAGCACACACAATGAAGACCAAGCCAGCTGTGAGGTGCTCACTGTGAAGAAGAAGGCAGGGGCCGTGACCTCAACCCCAAACAGGAACTCATCCAAGAGACGGTCCTCCCTTCCCAATGGGGAAGGGCTGCAGCTGAAGGAGAACTCGGAATCCGAGGGTGTTTCCTGCCACTATTGGTCGCTGTTTGACGGGCACGCGGGGTCCGGGGCCGCGGTGGTGGCGTCACGCCTGCTGCAGCACCACATCACGGAGCAGCTGCAGGACATCGTGGACATCCTGAAGAACTCCGCCGTCCTGCCCCCTACCTGCCTGGGGGAGGAGCCTGAGAACACGCCCGCCAACAGCCGGACTCTGACCCGGGCAGCCTCCCTGCGCGGAGGGGTGGGGGCCCCGGGCTCCCCCAGCACGCCCCCCACACGCTTCTTTACCGAGAAGAAGATTCCCCATGAGTGCCTGGTCATCGGAGCGCTTGAAAGTGCATTCAAGGAAATGGACCTACAGATAGAACGAGAGAGGAGTTCATATAATATATCTGGTGGCTGCACGGCCCTCATTGTGATTTGCCTTTTGGGGAAGCTGTATGTTGCAAATGCTGGGGATAGCAGGGCCATAATCATCAGAAATGGAGAAATTATCCCCATGTCTTCAGAATTTACCCCCGAGACGGAGCGCCAGCGACTTCAGTACCTGGCATTCATGCAGCCTCACTTGCTGGGAAATGAGTTCACACATTTGGAGTTTCCAAGGAGAGTACAGAGAAAGGAGCTTGGAAAGAAGATGCTCTACAGGGACTTTAATATGACAGGCTGGGCATACAAAACCATTGAGGATGAGGACTTGAAGTTCCCCCTTATATATGGAGAAGGCAAGAAGGCCCGGGTAATGGCAACTATTGGAGTGACCAGGGGACTTGGGGACCATGACCTGAAGGTGCATGACTCCAACATCTACATTAAACCATTCCTGTCTTCAGCTCCAGAGGTAAGAATCTACGATCTTTCAAAATATGATCATGGATCAGATGATGTGCTGATCTTGGCCACTGATGGACTCTGGGACGTTTTATCAAATGAAGAAGTAGCAGAAGCAATCACTCAGTTTCTTCCTAACTGTGATCCAGATGATCCTCACAGGTACACACTGGCAGCTCAGGACCTGGTGATGCGTGCCCGGGGTGTGCTGAAGGACAGAGGATGGCGGATATCTAATGACCGACTGGGCTCAGGAGACGACATTTCTGTATATGTCATTCCTTTAATACATGGAAACAAGCTGTCATGAGCGGCCGC
Amino Acid Sequence
View Sequence
MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTHLKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQEQTGGMLTRVKSAVANFMGGIMAGSSGSEHGGGSCGGSDLPLRFPYGRPEFLGLSQDEVECSADHIARPILILKETRRLPWATGYAEVINAGKSTHNEDQASCEVLTVKKKAGAVTSTPNRNSSKRRSSLPNGEGLQLKENSESEGVSCHYWSLFDGHAGSGAAVVASRLLQHHITEQLQDIVDILKNSAVLPPTCLGEEPENTPANSRTLTRAASLRGGVGAPGSPSTPPTRFFTEKKIPHECLVIGALESAFKEMDLQIERERSSYNISGGCTALIVICLLGKLYVANAGDSRAIIIRNGEIIPMSSEFTPETERQRLQYLAFMQPHLLGNEFTHLEFPRRVQRKELGKKMLYRDFNMTGWAYKTIEDEDLKFPLIYGEGKKARVMATIGVTRGLGDHDLKVHDSNIYIKPFLSSAPEVRIYDLSKYDHGSDDVLILATDGLWDVLSNEEVAEAITQFLPNCDPDDPHRYTLAAQDLVMRARGVLKDRGWRISNDRLGSGDDISVYVIPLIHGNKLS*
Vector Type
Bacterial
Antibiotic
Amp
Comments
linker after SENP cleavage deleted to make clone flush.
100042 R 1000 1001 1047 19007
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