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Plasmid
pET15b 6HIS SUMO PPM1H
Parent Plasmid
pET15b 6His SUMO
Expressed
6HIS SUMO PPM1H
DU Number
DU62790
Genbank
Q9ULR3.2
Tag
6His
SUMO
Species
Human
Sequence of Insert
View Sequence
GGATCCATGCTCACTCGAGTGAAATCTGCCGTGGCCAATTTCATGGGCGGCATCATGGCTGGCAGCTCAGGCTCCGAGCACGGCGGCGGCAGCTGCGGAGGCTCGGACCTGCCCCTGCGTTTCCCCTACGGGCGGCCAGAGTTCCTGGGGCTGTCTCAGGACGAGGTGGAGTGCAGCGCCGACCACATCGCCCGCCCCATCCTCATCCTCAAGGAGACTCGGCGGCTGCCCTGGGCCACTGGCTACGCAGAGGTTATCAATGCCGGGAAGAGCACACACAATGAAGACCAAGCCAGCTGTGAGGTGCTCACTGTGAAGAAGAAGGCAGGGGCCGTGACCTCAACCCCAAACAGGAACTCATCCAAGAGACGGTCCTCCCTTCCCAATGGGGAAGGGCTGCAGCTGAAGGAGAACTCGGAATCCGAGGGTGTTTCCTGCCACTATTGGTCGCTGTTTGACGGGCACGCGGGGTCCGGGGCCGCGGTGGTGGCGTCACGCCTGCTGCAGCACCACATCACGGAGCAGCTGCAGGACATCGTGGACATCCTGAAGAACTCCGCCGTCCTGCCCCCTACCTGCCTGGGGGAGGAGCCTGAGAACACGCCCGCCAACAGCCGGACTCTGACCCGGGCAGCCTCCCTGCGCGGAGGGGTGGGGGCCCCGGGCTCCCCCAGCACGCCCCCCACACGCTTCTTTACCGAGAAGAAGATTCCCCATGAGTGCCTGGTCATCGGAGCGCTTGAAAGTGCATTCAAGGAAATGGACCTACAGATAGAACGAGAGAGGAGTTCATATAATATATCTGGTGGCTGCACGGCCCTCATTGTGATTTGCCTTTTGGGGAAGCTGTATGTTGCAAATGCTGGGGATAGCAGGGCCATAATCATCAGAAATGGAGAAATTATCCCCATGTCTTCAGAATTTACCCCCGAGACGGAGCGCCAGCGACTTCAGTACCTGGCATTCATGCAGCCTCACTTGCTGGGAAATGAGTTCACACATTTGGAGTTTCCAAGGAGAGTACAGAGAAAGGAGCTTGGAAAGAAGATGCTCTACAGGGACTTTAATATGACAGGCTGGGCATACAAAACCATTGAGGATGAGGACTTGAAGTTCCCCCTTATATATGGAGAAGGCAAGAAGGCCCGGGTAATGGCAACTATTGGAGTGACCAGGGGACTTGGGGACCATGACCTGAAGGTGCATGACTCCAACATCTACATTAAACCATTCCTGTCTTCAGCTCCAGAGGTAAGAATCTACGATCTTTCAAAATATGATCATGGATCAGATGATGTGCTGATCTTGGCCACTGATGGACTCTGGGACGTTTTATCAAATGAAGAAGTAGCAGAAGCAATCACTCAGTTTCTTCCTAACTGTGATCCAGATGATCCTCACAGGTACACACTGGCAGCTCAGGACCTGGTGATGCGTGCCCGGGGTGTGCTGAAGGACAGAGGATGGCGGATATCTAATGACCGACTGGGCTCAGGAGACGACATTTCTGTATATGTCATTCCTTTAATACATGGAAACAAGCTGTCATGAGCGGCCGC
Amino Acid Sequence
View Sequence
MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTHLKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQEQTGGSMLTRVKSAVANFMGGIMAGSSGSEHGGGSCGGSDLPLRFPYGRPEFLGLSQDEVECSADHIARPILILKETRRLPWATGYAEVINAGKSTHNEDQASCEVLTVKKKAGAVTSTPNRNSSKRRSSLPNGEGLQLKENSESEGVSCHYWSLFDGHAGSGAAVVASRLLQHHITEQLQDIVDILKNSAVLPPTCLGEEPENTPANSRTLTRAASLRGGVGAPGSPSTPPTRFFTEKKIPHECLVIGALESAFKEMDLQIERERSSYNISGGCTALIVICLLGKLYVANAGDSRAIIIRNGEIIPMSSEFTPETERQRLQYLAFMQPHLLGNEFTHLEFPRRVQRKELGKKMLYRDFNMTGWAYKTIEDEDLKFPLIYGEGKKARVMATIGVTRGLGDHDLKVHDSNIYIKPFLSSAPEVRIYDLSKYDHGSDDVLILATDGLWDVLSNEEVAEAITQFLPNCDPDDPHRYTLAAQDLVMRARGVLKDRGWRISNDRLGSGDDISVYVIPLIHGNKLS*
Vector Type
Bacterial
Antibiotic
Amp
Comments
Needs linker after SENP cleavage deleted to make clone flush.
100042 R 1000 1001 1047 19007
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