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Plasmid
pLVX hygro GFP VPS35
Parent Plasmid
pLVX Hygro GFP
Expressed
GFP VPS35
DU Number
DU29494
Genbank
NM_018206
Tag
GFP
Species
Human
Sequence of Insert
View Sequence
ctcgagATGCCTACAACACAGCAGTCCCCTCAGGATGAGCAGGAAAAGCTCTTGGATGAAGCCATACAGGCTGTGAAGGTCCAGTCATTCCAAATGAAGAGATGCCTGGACAAAAACAAGCTTATGGATGCTCTAAAACATGCTTCTAATATGCTTGGTGAACTCCGGACTTCTATGTTATCACCAAAGAGTTACTATGAACTTTATATGGCCATTTCTGATGAACTGCACTACTTGGAGGTCTACCTGACAGATGAGTTTGCTAAAGGAAGGAAAGTGGCAGATCTCTACGAACTTGTACAGTATGCTGGAAACATTATCCCAAGGCTTTACCTTTTGATCACAGTTGGAGTTGTATATGTCAAGTCATTTCCTCAGTCCAGGAAGGATATTTTGAAAGATTTGGTAGAAATGTGCCGTGGTGTGCAACATCCCTTGAGGGGTCTGTTTCTTCGAAATTACCTTCTTCAGTGTACCAGAAATATCTTACCTGATGAAGGAGAGCCAACAGATGAAGAAACAACTGGTGACATCAGTGATTCCATGGATTTTGTACTGCTCAACTTTGCAGAAATGAACAAGCTCTGGGTGCGAATGCAGCATCAGGGACATAGCCGAGATAGAGAAAAAAGAGAACGAGAAAGACAAGAACTGAGAATTTTAGTGGGAACAAATTTGGTGCGCCTCAGTCAGTTGGAAGGTGTAAATGTGGAACGTTACAAACAGATTGTTTTGACTGGCATATTGGAGCAAGTTGTAAACTGTAGGGATGCTTTGGCTCAAGAATATCTCATGGAGTGTATTATTCAGGTTTTCCCTGATGAATTTCACCTCCAGACTTTGAATCCTTTTCTTCGGGCCTGTGCTGAGTTACACCAGAATGTAAATGTGAAGAACATAATCATTGCTTTAATTGATAGATTAGCTTTATTTGCTCACCGTGAAGATGGACCTGGAATCCCAGCGGATATTAAACTTTTTGATATATTTTCACAGCAGGTGGCTACAGTGATACAGTCTAGACAAGACATGCCTTCAGAGGATGTTGTATCTTTACAAGTCTCTCTGATTAATCTTGCCATGAAATGTTACCCTGATCGTGTGGACTATGTTGATAAAGTTCTAGAAACAACAGTGGAGATATTCAATAAGCTCAACCTTGAACATATTGCTACCAGTAGTGCAGTTTCAAAGGAACTCACCAGACTTTTGAAAATACCAGTTGACACTTACAACAATATTTTAACAGTCTTGAAATTAAAACATTTTCACCCACTCTTTGAGTACTTTGACTACGAGTCCAGAAAGAGCATGAGTTGTTATGTGCTTAGTAATGTTCTGGATTATAACACAGAAATTGTCTCTCAAGACCAGGTGGATTCCATAATGAATTTGGTATCCACGTTGATTCAAGATCAGCCAGATCAACCTGTAGAAGACCCTGATCCAGAAGATTTTGCTGATGAGCAGAGCCTTGTGGGCCGCTTCATTCATCTGCTGCGCTCTGAGGACCCTGACCAGCAGTACTTGATTTTGAACACAGCACGAAAACATTTTGGAGCTGGTGGAAATCAGCGGATTCGCTTCACACTGCCACCTTTGGTATTTGCAGCTTACCAGCTGGCTTTTCGATATAAAGAGAATTCTAAAGTGGATGACAAATGGGAAAAGAAATGCCAGAAGATTTTTTCATTTGCCCACCAGACTATCAGTGCTTTGATCAAAGCAGAGCTGGCAGAATTGCCCTTAAGACTTTTTCTTCAAGGAGCACTAGCTGCTGGGGAAATTGGTTTTGAAAATCATGAGACAGTCGCATATGAATTCATGTCCCAGGCATTTTCTCTGTATGAAGATGAAATCAGCGATTCCAAAGCACAGCTAGCTGCCATCACCTTGATCATTGGCACTTTTGAAAGGATGAAGTGCTTCAGTGAAGAGAATCATGAACCTCTGAGGACTCAGTGTGCCCTTGCTGCATCCAAACTTCTAAAGAAACCTGATCAGGGCCGAGCTGTGAGCACCTGTGCACATCTCTTCTGGTCTGGCAGAAACACGGACAAAAATGGGGAGGAGCTTCACGGAGGCAAGAGGGTAATGGAGTGCCTAAAAAAAGCTCTAAAAATAGCAAATCAGTGCATGGACCCCTCTCTACAAGTGCAGCTTTTTATAGAAATTCTGAACAGATATATCTATTTTTATGAAAAGGAAAATGATGCGGTAACAATTCAGGTTTTAAACCAGCTTATCCAAAAGATTCGAGAAGACCTCCCGAATCTTGAATCCAGTGAAGAAACAGAGCAGATTAACAAACATTTTCATAACACACTGGAGCATTTGCGCTTGCGGCGGGAATCACCAGAATCCGAGGGGCCAATTTATGAAGGTCTCATCCTTtaaggatcc
Amino Acid Sequence
View Sequence
MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKLEMPTTQQSPQ
DEQEKLLDEAIQAVKVQSFQMKRCLDKNKLMDALKHASNMLGELRTSMLS
PKSYYELYMAISDELHYLEVYLTDEFAKGRKVADLYELVQYAGNIIPRLY
LLITVGVVYVKSFPQSRKDILKDLVEMCRGVQHPLRGLFLRNYLLQCTRN
ILPDEGEPTDEETTGDISDSMDFVLLNFAEMNKLWVRMQHQGHSRDREKR
ERERQELRILVGTNLVRLSQLEGVNVERYKQIVLTGILEQVVNCRDALAQ
EYLMECIIQVFPDEFHLQTLNPFLRACAELHQNVNVKNIIIALIDRLALF
AHREDGPGIPADIKLFDIFSQQVATVIQSRQDMPSEDVVSLQVSLINLAM
KCYPDRVDYVDKVLETTVEIFNKLNLEHIATSSAVSKELTRLLKIPVDTY
NNILTVLKLKHFHPLFEYFDYESRKSMSCYVLSNVLDYNTEIVSQDQVDS
IMNLVSTLIQDQPDQPVEDPDPEDFADEQSLVGRFIHLLRSEDPDQQYLI
LNTARKHFGAGGNQRIRFTLPPLVFAAYQLAFRYKENSKVDDKWEKKCQK
IFSFAHQTISALIKAELAELPLRLFLQGALAAGEIGFENHETVAYEFMSQ
AFSLYEDEISDSKAQLAAITLIIGTFERMKCFSEENHEPLRTQCALAASK
LLKKPDQGRAVSTCAHLFWSGRNTDKNGEELHGGKRVMECLKKALKIANQ
CMDPSLQVQLFIEILNRYIYFYEKENDAVTIQVLNQLIQKIREDLPNLES
SEETEQINKHFHNTLEHLRLRRESPESEGPIYEGLIL*
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKLEMPTTQQSPQ
DEQEKLLDEAIQAVKVQSFQMKRCLDKNKLMDALKHASNMLGELRTSMLS
PKSYYELYMAISDELHYLEVYLTDEFAKGRKVADLYELVQYAGNIIPRLY
LLITVGVVYVKSFPQSRKDILKDLVEMCRGVQHPLRGLFLRNYLLQCTRN
ILPDEGEPTDEETTGDISDSMDFVLLNFAEMNKLWVRMQHQGHSRDREKR
ERERQELRILVGTNLVRLSQLEGVNVERYKQIVLTGILEQVVNCRDALAQ
EYLMECIIQVFPDEFHLQTLNPFLRACAELHQNVNVKNIIIALIDRLALF
AHREDGPGIPADIKLFDIFSQQVATVIQSRQDMPSEDVVSLQVSLINLAM
KCYPDRVDYVDKVLETTVEIFNKLNLEHIATSSAVSKELTRLLKIPVDTY
NNILTVLKLKHFHPLFEYFDYESRKSMSCYVLSNVLDYNTEIVSQDQVDS
IMNLVSTLIQDQPDQPVEDPDPEDFADEQSLVGRFIHLLRSEDPDQQYLI
LNTARKHFGAGGNQRIRFTLPPLVFAAYQLAFRYKENSKVDDKWEKKCQK
IFSFAHQTISALIKAELAELPLRLFLQGALAAGEIGFENHETVAYEFMSQ
AFSLYEDEISDSKAQLAAITLIIGTFERMKCFSEENHEPLRTQCALAASK
LLKKPDQGRAVSTCAHLFWSGRNTDKNGEELHGGKRVMECLKKALKIANQ
CMDPSLQVQLFIEILNRYIYFYEKENDAVTIQVLNQLIQKIREDLPNLES
SEETEQINKHFHNTLEHLRLRRESPESEGPIYEGLIL*
Vector Type
Mammalian
Antibiotic
Amp
Comments
Parental 62134
100042 R 1000 1001 1047 19007
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