RILPL1

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Plasmid
pET15b 6HIS SUMO RILPL1 M1-T295
Parent Plasmid
pET15b 6His SUMO
Expressed
6HIS SUMO RILPL1 M1-T295
DU Number
DU26804
Genbank
NM_178314.4
Tag
6His
SUMO
Sequence of Insert
ATGGAGGAGGAGCGGGGGTCGGCGCTGGCGGCCGAGTCGGCGCTGGAGAAGAACGTGGCCGAGCTGACCGTCATGGACGTGTACGACATCGCGTCGCTTGTGGGCCACGAGTTCGAGCGGGTCATTGACCAGCACGGCTGCGAGGCCATCGCGCGCCTCATGCCCAAGGTCGTGCGCGTCCTGGAGATCCTGGAGGTGCTGGTCAGCCGCCACCACGTCGCGCCCGAGCTGGACGAGCTGCGCCTGGAGCTGGACCGCCTGCGCCTGGAGAGGATGGACCGCATCGAGAAGGAGCGCAAGCACCAGAAGGAGCTGGAGCTGGTGGAGGATGTGTGGCGAGGGGAGGCGCAGGACCTCCTCTCCCAGATCGCCCAGCTGCAGGAGGAGAACAAGCAGCTCATGACCAACCTCTCCCACAAGGATGTCAACTTCTCAGAGGAGGAGTTCCAGAAGCATGAAGGCATGTCAGAGCGGGAGCGACAGGTGATGAAGAAGCTGAAGGAGGTGGTGGACAAACAACGCGACGAGATCCGCGCCAAGGACAGGGAGCTGGGCCTGAAAAATGAGGACGTTGAGGCTTTACAGCAGCAGCAGACACGGCTGATGAAGATCAACCATGACCTTCGGCACCGGGTCACGGTGGTGGAGGCCCAGGGGAAAGCCCTGATCGAACAGAAGGTGGAGCTGGAGGCAGACCTGCAGACCAAGGAGCAGGAGATGGGCAGCCTGCGAGCAGAGCTGGGGAAGTTGCGAGAGAGGCTGCAGGGGGAGCACAGCCAGAATGGGGAGGAGGAGCCTGAGACGGAGCCGGTGGGAGAGGAGAGCATCTCCGACGCAGAGAAGGTGGCCATGGATCTCAAGGACCCCAACCGCCCCCGGTTCACCTGACAGGAGCTGCGGGACGTGCTGCACGAGAGGAACGAGCTCAAGTCCAAGGTGTTCTTGCTGCAGGAGGAGCTGGCTTACTATAAGAGTGAAGAAATGGAAGAGGAAAACCGAATACCCCAACCCCCACCCATCGCCCACCCGAGGACGTCCCCCCAGCCGGAGTCGGGCATCAAGCGACTGTTTAGCTTCTTCTCCCGAGATAAGAAGCGCCTGGCCAACACACAGAGAAACGTGCACATCCAGGAGTCCTTTGGACAGTGGGCAAACACCCACCGCGATGACGGTTACACAGAGCAAGGACAGGAAGCCCTGCAGCATCTGTGAGCGGCCGC
Amino Acid Sequence
MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTHLKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQEQTGGMEEERGSALAAESALEKNVAELTVMDVYDIASLVGHEFERVIDQHGCEAIARLMPKVVRVLEILEVLVSRHHVAPELDELRLELDRLRLERMDRIEKERKHQKELELVEDVWRGEAQDLLSQIAQLQEENKQLMTNLSHKDVNFSEEEFQKHEGMSERERQVMKKLKEVVDKQRDEIRAKDRELGLKNEDVEALQQQQTRLMKINHDLRHRVTVVEAQGKALIEQKVELEADLQTKEQEMGSLRAELGKLRERLQGEHSQNGEEEPETEPVGEESISDAEKVAMDLKDPNRPRFT*
Vector Type
Bacterial
Antibiotic
Amp
Comments
Linker after SENP Cleavage deleted.
Stop inserted by mutagenesis at amino acid 295
Silent mutation N143
100042 R 1000 1001 1047 19007
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