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Plasmid
pET15 6HIS SUMO-JIP4
Parent Plasmid
pET15 6HIS SUMO
Expressed
6HIS SUMO-JIP4
DU Number
DU28521
Genbank
NM_001130528.2
Tag
6His
SUMO
Species
Human
Sequence of Insert
View Sequence
ggatccATGGAGCTGGAGGACGGTGTGGTGTATCAGGAGGAGCCCGGCGG
CTCCGGGGCCGTGATGTCGGAGCGGGTGTCCGGCCTGGCCGGCTCCATCT
ACCGCGAGTTCGAGCGGCTTATCGGGCGCTATGACGAGGAGGTGGTCAAA
GAGCTGATGCCGCTGGTGGTGGCTGTGCTGGAGAACCTGGACTCGGTGTT
CGCGCAGGACCAGGAGCACCAGGTGGAGCTGGAGCTGCTGCGGGACGACA
ACGAGCAGCTCATCACCCAGTACGAGCGGGAGAAGGCGCTGCGCAAGCAC
GCTGAGGAGAAATTCATTGAATTTGAAGACTCTCAAGAACAGGAAAAAAA
GGACTTACAGACCCGAGTGGAATCTTTAGAATCTCAAACAAGACAACTTG
AGCTGAAAGCGAAAAACTATGCTGACCAGATTAGCAGACTTGAAGAAAGA
GAAGCAGAACTGAAGAAGGAATATAATGCATTACATCAAAGACACACTGA
GATGATCCATAATTATATGGAACATTTAGAAAGAACAAAACTTCATCAGC
TCTCAGGGAGTGATCAACTAGAATCCACAGCTCATAGTAGAATTAGAAAA
GAACGCCCTATATCATTAGGAATTTTCCCATTACCTGCTGGAGATGGATT
GCTTACACCTGATGCTCAGAAAGGAGGAGAGACCCCTGGATCTGAGCAAT
GGAAATTTCAGGAATTAAGTCAACCACGTTCTCATACCAGCCTGAAGGTC
AGCAATAGTCCTGAACCTCAGAAGGCTGTAGAACAGGAGGATGAGCTTTC
TGATGTTAGCCAAGGCGGATCTAAAGCTACCACTCCAGCATCAACAGCTA
ATTCAGATGTGGCAACAATTCCTACTGATACTCCCTTAAAGGAAGAAAAC
GAAGGATTTGTGAAGGTTACAGATGCGCCAAATAAATCAGAGATAAGCAA
ACACATTGAAGTACAGGTAGCCCAGGAAACTAGAAATGTATCTACTGGCT
CTGCTGAAAATGAAGAAAAGTCAGAAGTTCAAGCAATCATCGAATCTACT
CCTGAGCTGGATATGGACAAAGATCTCAGTGGATATAAAGGTTCAAGCAC
TCCCACCAAAGGCATAGAGAACAAAGCTTTTGATCGCAATACAGAATCTC
TCTTTGAAGAACTGTCTTCAGCTGGCTCAGGCCTAATAGGAGATGTGGAT
GAAGGAGCAGATTTACTAGGAATGGGTCGGGAAGTTGAGAATCTTATATT
AGAAAATACACAACTGTTGGAAACCAAAAATGCTTTGAACATAGTGAAGA
ATGATTTGATAGCAAAAGTGGATGAACTGACCTGTGAGAAAGATGTGCTG
CAAGGGGAATTGGAGGCTGTGAAGCAAGCCAAACTGAAACTAGAGGAAAA
GAACAGAGAATTGGAGGAAGAGCTTAGGAAAGCTCGGGCAGAAGCTGAAG
ATGCAAGGCAAAAAGCAAAAGATGACGATGATAGTGATATTCCCACAGCC
CAGAGGAAACGGTTTACTAGAGTAGAAATGGCCCGTGTTCTCATGGAGCG
AAACCAGTATAAAGAGAGATTGATGGAGCTTCAGGAAGCTGTTCGATGGA
CAGAGATGATTCGGGCATCACGAGAAAATCCAGCCATGCAGGAAAAAAAA
AGGTCAAGCATTTGGCAGTTTTTCAGCCGACTTTTCAGCTCCTCAAGTAA
CACGACTAAGAAGCCTGAACCACCTGTTAATCTGAAGTACAATGCACCCA
CGTCTCATGTTACTCCGTCCGTCAAGAAAAGAAGCAGCACCTTATCTCAG
CTCCCTGGGGATAAGTCCAAAGCCTTTGATTTCCTTAGTGAAGAAACTGA
AGCTAGTTTAGCCTCACGCAGAGAACAAAAGAGAGAGCAGTATCGTCAGG
TAAAAGCACATGTTCAGAAGGAAGACGGTAGAGTGCAGGCTTTTGGCTGG
AGTCTGCCTCAGAAGTACAAACAGGTAACCAATGGTCAAGGTGAAAATAA
GATGAAAAATTTACCTGTGCCTGTCTATCTCAGACCTCTGGATGAAAAAG
ATACATCAATGAAGCTGTGGTGTGCTGTTGGAGTCAATTTATCTGGTGGG
AAGACCAGAGATGGTGGTTCTGTTGTTGGAGCAAGTGTATTTTACAAGGA
TGTTGCTGGTTTGGATACAGAAGGCAGTAAACAGCGAAGTGCCTCTCAGA
GTAGTTTAGATAAGTTAGATCAGGAACTTAAGGAACAGCAGAAGGAGTTA
AAAAATCAAGAAGAATTATCCAGTCTAGTTTGGATCTGTACCAGCACTCA
TTCGGCTACAAAAGTTCTTATTATTGATGCTGTTCAACCTGGCAACATCC
TAGACAGTTTCACTGTTTGCAACTCTCATGTTCTGTGCATTGCAAGTGTG
CCAGGTGCACGAGAAACAGACTACCCTGCAGGAGAAGATCTTTCAGAATC
TGGTCAGGTAGACAAAGCATCTTTATGTGGAAGTATGACAAGCAACAGCT
CAGCAGAGACAGACAGCCTGTTAGGAGGCATCACAGTGGTTGGTTGTTCT
GCAGAAGGTGTGACGGGAGCTGCCACTTCCCCTAGTACAAATGGTGCTTC
TCCAGTGATGGATAAACCACCAGAAATGGAAGCAGAAAATAGTGAGGTTG
ATGAAAATGTTCCAACAGCAGAAGAAGCAACTGAAGCTACAGAAGGGAAT
GCGGGGTCAGCTGAAGACACAGTGGACATCTCCCAAACTGGCGTCTACAC
AGAGCATGTCTTTACAGATCCTTTGGGAGTTCAGATCCCAGAAGACCTCT
CCCCAGTGTATCAGTCGAGCAATGACTCAGATGCATATAAAGATCAAATA
TCAGTACTGCCAAATGAACAAGACTTGGTGAGAGAAGAAGCCCAGAAAAT
GAGTAGTCTTTTACCAACTATGTGGCTTGGAGCTCAAAATGGCTGTTTGT
ATGTCCATTCATCTGTAGCCCAGTGGAGGAAATGTCTCCATTCCATTAAA
CTTAAAGATTCGATTCTCAGTATTGTACACGTGAAGGGAATCGTGTTAGT
AGCCCTGGCTGACGGCACCCTTGCAATCTTTCACAGAGGAGTGGATGGGC
AGTGGGATTTGTCAAACTATCACCTCTTAGACCTTGGACGGCCTCATCAT
TCCATCCGTTGCATGACTGTGGTACATGACAAAGTCTGGTGTGGCTATAG
GAACAAAATCTATGTGGTGCAGCCAAAGGCCATGAAAATAGAGAAATCTT
TTGATGCACATCCCAGGAAGGAGAGCCAAGTGCGACAGCTTGCGTGGGTG
GGGGATGGCGTGTGGGTCTCCATTCGCTTGGATTCTACGCTCCGTCTCTA
TCATGCACACACTTATCAACATCTACAGGATGTGGACATTGAGCCTTATG
TAAGCAAAATGTTAGGTACTGGAAAACTGGGCTTCTCTTTTGTGAGAATT
ACAGCTCTTATGGTGTCTTGTAATCGTTTGTGGGTGGGGACAGGAAATGG
TGTCATTATCTCCATCCCATTGACAGAAACAAATAAAACCTCAGGTGTAC
CAGGAAATCGTCCTGGAAGTGTAATCCGTGTATATGGTGATGAAAACAGT
GATAAAGTGACTCCAGGGACATTTATACCCTATTGTTCAATGGCACATGC
ACAGCTTTGCTTCCATGGGCACCGGGATGCTGTGAAATTCTTTGTGGCAG
TCCCAGGTCAAGTCATCAGCCCACAAAGTAGCAGTAGTGGCACGGATCTG
ACGGGTGACAAAGCAGGGCCATCTGCACAGGAGCCTGGTAGTCAGACGCC
CTTGAAGTCTATGCTTGTCATCAGTGGAGGAGAGGGCTACATCGACTTCC
GAATGGGTGATGAAGGTGGAGAATCAGAACTTCTTGGAGAGGATCTTCCA
CTTGAACCTTCTGTCACCAAAGCAGAAAGGAGTCACTTGATAGTGTGGCA
AGTGATGTATGGCAATGAGTGAgcggccgc
CTCCGGGGCCGTGATGTCGGAGCGGGTGTCCGGCCTGGCCGGCTCCATCT
ACCGCGAGTTCGAGCGGCTTATCGGGCGCTATGACGAGGAGGTGGTCAAA
GAGCTGATGCCGCTGGTGGTGGCTGTGCTGGAGAACCTGGACTCGGTGTT
CGCGCAGGACCAGGAGCACCAGGTGGAGCTGGAGCTGCTGCGGGACGACA
ACGAGCAGCTCATCACCCAGTACGAGCGGGAGAAGGCGCTGCGCAAGCAC
GCTGAGGAGAAATTCATTGAATTTGAAGACTCTCAAGAACAGGAAAAAAA
GGACTTACAGACCCGAGTGGAATCTTTAGAATCTCAAACAAGACAACTTG
AGCTGAAAGCGAAAAACTATGCTGACCAGATTAGCAGACTTGAAGAAAGA
GAAGCAGAACTGAAGAAGGAATATAATGCATTACATCAAAGACACACTGA
GATGATCCATAATTATATGGAACATTTAGAAAGAACAAAACTTCATCAGC
TCTCAGGGAGTGATCAACTAGAATCCACAGCTCATAGTAGAATTAGAAAA
GAACGCCCTATATCATTAGGAATTTTCCCATTACCTGCTGGAGATGGATT
GCTTACACCTGATGCTCAGAAAGGAGGAGAGACCCCTGGATCTGAGCAAT
GGAAATTTCAGGAATTAAGTCAACCACGTTCTCATACCAGCCTGAAGGTC
AGCAATAGTCCTGAACCTCAGAAGGCTGTAGAACAGGAGGATGAGCTTTC
TGATGTTAGCCAAGGCGGATCTAAAGCTACCACTCCAGCATCAACAGCTA
ATTCAGATGTGGCAACAATTCCTACTGATACTCCCTTAAAGGAAGAAAAC
GAAGGATTTGTGAAGGTTACAGATGCGCCAAATAAATCAGAGATAAGCAA
ACACATTGAAGTACAGGTAGCCCAGGAAACTAGAAATGTATCTACTGGCT
CTGCTGAAAATGAAGAAAAGTCAGAAGTTCAAGCAATCATCGAATCTACT
CCTGAGCTGGATATGGACAAAGATCTCAGTGGATATAAAGGTTCAAGCAC
TCCCACCAAAGGCATAGAGAACAAAGCTTTTGATCGCAATACAGAATCTC
TCTTTGAAGAACTGTCTTCAGCTGGCTCAGGCCTAATAGGAGATGTGGAT
GAAGGAGCAGATTTACTAGGAATGGGTCGGGAAGTTGAGAATCTTATATT
AGAAAATACACAACTGTTGGAAACCAAAAATGCTTTGAACATAGTGAAGA
ATGATTTGATAGCAAAAGTGGATGAACTGACCTGTGAGAAAGATGTGCTG
CAAGGGGAATTGGAGGCTGTGAAGCAAGCCAAACTGAAACTAGAGGAAAA
GAACAGAGAATTGGAGGAAGAGCTTAGGAAAGCTCGGGCAGAAGCTGAAG
ATGCAAGGCAAAAAGCAAAAGATGACGATGATAGTGATATTCCCACAGCC
CAGAGGAAACGGTTTACTAGAGTAGAAATGGCCCGTGTTCTCATGGAGCG
AAACCAGTATAAAGAGAGATTGATGGAGCTTCAGGAAGCTGTTCGATGGA
CAGAGATGATTCGGGCATCACGAGAAAATCCAGCCATGCAGGAAAAAAAA
AGGTCAAGCATTTGGCAGTTTTTCAGCCGACTTTTCAGCTCCTCAAGTAA
CACGACTAAGAAGCCTGAACCACCTGTTAATCTGAAGTACAATGCACCCA
CGTCTCATGTTACTCCGTCCGTCAAGAAAAGAAGCAGCACCTTATCTCAG
CTCCCTGGGGATAAGTCCAAAGCCTTTGATTTCCTTAGTGAAGAAACTGA
AGCTAGTTTAGCCTCACGCAGAGAACAAAAGAGAGAGCAGTATCGTCAGG
TAAAAGCACATGTTCAGAAGGAAGACGGTAGAGTGCAGGCTTTTGGCTGG
AGTCTGCCTCAGAAGTACAAACAGGTAACCAATGGTCAAGGTGAAAATAA
GATGAAAAATTTACCTGTGCCTGTCTATCTCAGACCTCTGGATGAAAAAG
ATACATCAATGAAGCTGTGGTGTGCTGTTGGAGTCAATTTATCTGGTGGG
AAGACCAGAGATGGTGGTTCTGTTGTTGGAGCAAGTGTATTTTACAAGGA
TGTTGCTGGTTTGGATACAGAAGGCAGTAAACAGCGAAGTGCCTCTCAGA
GTAGTTTAGATAAGTTAGATCAGGAACTTAAGGAACAGCAGAAGGAGTTA
AAAAATCAAGAAGAATTATCCAGTCTAGTTTGGATCTGTACCAGCACTCA
TTCGGCTACAAAAGTTCTTATTATTGATGCTGTTCAACCTGGCAACATCC
TAGACAGTTTCACTGTTTGCAACTCTCATGTTCTGTGCATTGCAAGTGTG
CCAGGTGCACGAGAAACAGACTACCCTGCAGGAGAAGATCTTTCAGAATC
TGGTCAGGTAGACAAAGCATCTTTATGTGGAAGTATGACAAGCAACAGCT
CAGCAGAGACAGACAGCCTGTTAGGAGGCATCACAGTGGTTGGTTGTTCT
GCAGAAGGTGTGACGGGAGCTGCCACTTCCCCTAGTACAAATGGTGCTTC
TCCAGTGATGGATAAACCACCAGAAATGGAAGCAGAAAATAGTGAGGTTG
ATGAAAATGTTCCAACAGCAGAAGAAGCAACTGAAGCTACAGAAGGGAAT
GCGGGGTCAGCTGAAGACACAGTGGACATCTCCCAAACTGGCGTCTACAC
AGAGCATGTCTTTACAGATCCTTTGGGAGTTCAGATCCCAGAAGACCTCT
CCCCAGTGTATCAGTCGAGCAATGACTCAGATGCATATAAAGATCAAATA
TCAGTACTGCCAAATGAACAAGACTTGGTGAGAGAAGAAGCCCAGAAAAT
GAGTAGTCTTTTACCAACTATGTGGCTTGGAGCTCAAAATGGCTGTTTGT
ATGTCCATTCATCTGTAGCCCAGTGGAGGAAATGTCTCCATTCCATTAAA
CTTAAAGATTCGATTCTCAGTATTGTACACGTGAAGGGAATCGTGTTAGT
AGCCCTGGCTGACGGCACCCTTGCAATCTTTCACAGAGGAGTGGATGGGC
AGTGGGATTTGTCAAACTATCACCTCTTAGACCTTGGACGGCCTCATCAT
TCCATCCGTTGCATGACTGTGGTACATGACAAAGTCTGGTGTGGCTATAG
GAACAAAATCTATGTGGTGCAGCCAAAGGCCATGAAAATAGAGAAATCTT
TTGATGCACATCCCAGGAAGGAGAGCCAAGTGCGACAGCTTGCGTGGGTG
GGGGATGGCGTGTGGGTCTCCATTCGCTTGGATTCTACGCTCCGTCTCTA
TCATGCACACACTTATCAACATCTACAGGATGTGGACATTGAGCCTTATG
TAAGCAAAATGTTAGGTACTGGAAAACTGGGCTTCTCTTTTGTGAGAATT
ACAGCTCTTATGGTGTCTTGTAATCGTTTGTGGGTGGGGACAGGAAATGG
TGTCATTATCTCCATCCCATTGACAGAAACAAATAAAACCTCAGGTGTAC
CAGGAAATCGTCCTGGAAGTGTAATCCGTGTATATGGTGATGAAAACAGT
GATAAAGTGACTCCAGGGACATTTATACCCTATTGTTCAATGGCACATGC
ACAGCTTTGCTTCCATGGGCACCGGGATGCTGTGAAATTCTTTGTGGCAG
TCCCAGGTCAAGTCATCAGCCCACAAAGTAGCAGTAGTGGCACGGATCTG
ACGGGTGACAAAGCAGGGCCATCTGCACAGGAGCCTGGTAGTCAGACGCC
CTTGAAGTCTATGCTTGTCATCAGTGGAGGAGAGGGCTACATCGACTTCC
GAATGGGTGATGAAGGTGGAGAATCAGAACTTCTTGGAGAGGATCTTCCA
CTTGAACCTTCTGTCACCAAAGCAGAAAGGAGTCACTTGATAGTGTGGCA
AGTGATGTATGGCAATGAGTGAgcggccgc
Amino Acid Sequence
View Sequence
MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTH
LKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGMELEDGVVYQEEPGGSGAVMSERVSGLAGSIYREFERLIGRYDEEV
VKELMPLVVAVLENLDSVFAQDQEHQVELELLRDDNEQLITQYEREKALR
KHAEEKFIEFEDSQEQEKKDLQTRVESLESQTRQLELKAKNYADQISRLE
EREAELKKEYNALHQRHTEMIHNYMEHLERTKLHQLSGSDQLESTAHSRI
RKERPISLGIFPLPAGDGLLTPDAQKGGETPGSEQWKFQELSQPRSHTSL
KVSNSPEPQKAVEQEDELSDVSQGGSKATTPASTANSDVATIPTDTPLKE
ENEGFVKVTDAPNKSEISKHIEVQVAQETRNVSTGSAENEEKSEVQAIIE
STPELDMDKDLSGYKGSSTPTKGIENKAFDRNTESLFEELSSAGSGLIGD
VDEGADLLGMGREVENLILENTQLLETKNALNIVKNDLIAKVDELTCEKD
VLQGELEAVKQAKLKLEEKNRELEEELRKARAEAEDARQKAKDDDDSDIP
TAQRKRFTRVEMARVLMERNQYKERLMELQEAVRWTEMIRASRENPAMQE
KKRSSIWQFFSRLFSSSSNTTKKPEPPVNLKYNAPTSHVTPSVKKRSSTL
SQLPGDKSKAFDFLSEETEASLASRREQKREQYRQVKAHVQKEDGRVQAF
GWSLPQKYKQVTNGQGENKMKNLPVPVYLRPLDEKDTSMKLWCAVGVNLS
GGKTRDGGSVVGASVFYKDVAGLDTEGSKQRSASQSSLDKLDQELKEQQK
ELKNQEELSSLVWICTSTHSATKVLIIDAVQPGNILDSFTVCNSHVLCIA
SVPGARETDYPAGEDLSESGQVDKASLCGSMTSNSSAETDSLLGGITVVG
CSAEGVTGAATSPSTNGASPVMDKPPEMEAENSEVDENVPTAEEATEATE
GNAGSAEDTVDISQTGVYTEHVFTDPLGVQIPEDLSPVYQSSNDSDAYKD
QISVLPNEQDLVREEAQKMSSLLPTMWLGAQNGCLYVHSSVAQWRKCLHS
IKLKDSILSIVHVKGIVLVALADGTLAIFHRGVDGQWDLSNYHLLDLGRP
HHSIRCMTVVHDKVWCGYRNKIYVVQPKAMKIEKSFDAHPRKESQVRQLA
WVGDGVWVSIRLDSTLRLYHAHTYQHLQDVDIEPYVSKMLGTGKLGFSFV
RITALMVSCNRLWVGTGNGVIISIPLTETNKTSGVPGNRPGSVIRVYGDE
NSDKVTPGTFIPYCSMAHAQLCFHGHRDAVKFFVAVPGQVISPQSSSSGT
DLTGDKAGPSAQEPGSQTPLKSMLVISGGEGYIDFRMGDEGGESELLGED
LPLEPSVTKAERSHLIVWQVMYGNE*
LKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGMELEDGVVYQEEPGGSGAVMSERVSGLAGSIYREFERLIGRYDEEV
VKELMPLVVAVLENLDSVFAQDQEHQVELELLRDDNEQLITQYEREKALR
KHAEEKFIEFEDSQEQEKKDLQTRVESLESQTRQLELKAKNYADQISRLE
EREAELKKEYNALHQRHTEMIHNYMEHLERTKLHQLSGSDQLESTAHSRI
RKERPISLGIFPLPAGDGLLTPDAQKGGETPGSEQWKFQELSQPRSHTSL
KVSNSPEPQKAVEQEDELSDVSQGGSKATTPASTANSDVATIPTDTPLKE
ENEGFVKVTDAPNKSEISKHIEVQVAQETRNVSTGSAENEEKSEVQAIIE
STPELDMDKDLSGYKGSSTPTKGIENKAFDRNTESLFEELSSAGSGLIGD
VDEGADLLGMGREVENLILENTQLLETKNALNIVKNDLIAKVDELTCEKD
VLQGELEAVKQAKLKLEEKNRELEEELRKARAEAEDARQKAKDDDDSDIP
TAQRKRFTRVEMARVLMERNQYKERLMELQEAVRWTEMIRASRENPAMQE
KKRSSIWQFFSRLFSSSSNTTKKPEPPVNLKYNAPTSHVTPSVKKRSSTL
SQLPGDKSKAFDFLSEETEASLASRREQKREQYRQVKAHVQKEDGRVQAF
GWSLPQKYKQVTNGQGENKMKNLPVPVYLRPLDEKDTSMKLWCAVGVNLS
GGKTRDGGSVVGASVFYKDVAGLDTEGSKQRSASQSSLDKLDQELKEQQK
ELKNQEELSSLVWICTSTHSATKVLIIDAVQPGNILDSFTVCNSHVLCIA
SVPGARETDYPAGEDLSESGQVDKASLCGSMTSNSSAETDSLLGGITVVG
CSAEGVTGAATSPSTNGASPVMDKPPEMEAENSEVDENVPTAEEATEATE
GNAGSAEDTVDISQTGVYTEHVFTDPLGVQIPEDLSPVYQSSNDSDAYKD
QISVLPNEQDLVREEAQKMSSLLPTMWLGAQNGCLYVHSSVAQWRKCLHS
IKLKDSILSIVHVKGIVLVALADGTLAIFHRGVDGQWDLSNYHLLDLGRP
HHSIRCMTVVHDKVWCGYRNKIYVVQPKAMKIEKSFDAHPRKESQVRQLA
WVGDGVWVSIRLDSTLRLYHAHTYQHLQDVDIEPYVSKMLGTGKLGFSFV
RITALMVSCNRLWVGTGNGVIISIPLTETNKTSGVPGNRPGSVIRVYGDE
NSDKVTPGTFIPYCSMAHAQLCFHGHRDAVKFFVAVPGQVISPQSSSSGT
DLTGDKAGPSAQEPGSQTPLKSMLVISGGEGYIDFRMGDEGGESELLGED
LPLEPSVTKAERSHLIVWQVMYGNE*
Vector Type
Bacterial
Antibiotic
Amp
Comments
Accession number (uniprotKB): O60271. Isoform1 (O60271-1).
100042 R 1000 1001 1047 19007
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