RAB8B

Plasmid
pMK-RQ RAB8B Nter HA donor
Parent Plasmid
pMK-RQ
DU Number
DU48994
Genbank
NM_016530.2
Species
Human
Sequence of Insert
gcggccgcAAGGAAAGAAAGCATCTCTGCTCCAGGTCTCGGCGGAAGAGATGCGGGAACTACGGGGCTCTGATTCTGAGGTCCTGGATTTGGAAGGCGTCTGCACACAAAGAAATAACAAAGTTAAGTTCCCGCGGCTCGACCAGCAGAGTACGAAACGGGGCCTCCCTTCCCAGCTCGGGTGGAAACGCCTCCACCCTGTCACCCCGCCCATAGCAGGTCTTTTATCCGCTGGAGCCGCCTGGACAGTCCTCGCGGGGCACGCCCGAGGCTCCCCGGAGGAACTACAAGTCCCATGAAGTCCCGGGGCGCGGGCGGTGATGACGAAGCCCTCGCCGATTGGCCGCACGGGGCGGGGCGCGCAGGCCTTCTCCGAGAGGAGGGGCGGGGCGTGGGGCGGGGCGAGCAGCGGCGGATTGGCGGGCACGCCCCCTCGCCCGCGGCCCCCTCCCCGCCTCTCTCCACCGCCTCCTCTGGCTCCCCGGTCAGAGGGCCGGAGCGAGAAGATGTACCCATACGATGTGCCAGATTACGCCGCGAAGACGTACGATTATCTCTTCAAGCTCCTGttaATCGGtGACagcGGGGTAGGCAAGACCTGCCTCCTGTTCCGCTTCTCAGAGGACGCCTTCAACACCACCTTCATCTCCACCATCGGTGAGGGAGGGGCCGCGGCCCGGGACCGGGTAGAGAATTGGGGGGCGGGTACTAGGGGACGGGGAAAGGGCGGGGGGCGCTTGGGAGCCCGGGAGAGGAGACCCCGGGGACTGCAAGGTGGCGGGGGCACTGAGAGGGGCGAGGGCGTGAGGGAGAGGGGCGAGGGCGTGAGGGAGAGAGGCGAGCGGGAACGTGAGGCTAAGGGCGAAGGGGGAACTTGGGGGCAAAGGAACGGGAAGGGGAACAGAGGAACAAGGACTAGGGGTATTGGGGGAACGAGAGGGTTCGGAGACTGACAGGGAGGGGTCTTTGAGGGACAAAATGTGTTGGGGGGAAGACTCAGGAAATCTCATGTAGAGAGAAGGGGATAGTGGACGTGCCAATGATTAAAAGACAGGAGAGGAAGGAAATGTGAAGGGGGAAATGAGcggccgc
Vector Type
Other
Antibiotic
Kan
Comments
CRISPR donor plasmid. Designed to KI an HA tag to the N-terminus of RAB8B when co-transfected with guides 48935 and 48949. Donor sequence shown as sequence of insert. Positives may be enriched for with puromycin prior to further selection by western blotting and FACs. Note this system does not require induction with Tet/Dox. May be used in any cell-line, contains several silent mutations to prevent binding of guides.
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