RAB1B

Plasmid
pMA RAB1B Nter HA donor
Parent Plasmid
pMA
DU Number
DU48991
Genbank
NM_030981.2
Species
Human
Sequence of Insert
gcggccgcAGTTAGGACTGCCCAGTACACGAAGAACCCTGGACAAATGTACTTTTATTAGTCTTACTAGAACCCACATTCTCATTTAATGTTCACAACGTGGGTATTGGGAGGGCAAGGATGACTACCTCCGTCAGACAGATGTGAAACGTGCCAAGCCCAGCCCTAAATTGAGCTTTCTAAACACCGAGTCGCGGTCAGGCTTTCCAGCACTGTCCTCAAGCCTGAGTGCGTCCGGAGTGCAAACTACAAGCCCCAGAAGGCAATGCGCCAGCAGTGATGGGAGTGGGCGCGGAGGCGCGGGCTCCCCGACTACGCCTGCGCAGCGGGGCTGAGTTGGAGAACGTCTTAGACTACAAGTTCCGGGAATCAGCGCGGCGAGGCCTGAGAAGAAGACGTTTGCGCCTGCGCGTCGCGGGCGGGGCCTCTGGGGCGGAGCGGCCACCATCTTGGAACGGGAGGCGGAGCAGAGTCGACTGGGAGCGACCGAGCGGGCCGCCGCCGCCGCCATGTACCCATACGATGTGCCAGATTACGCCAACCCaGAgTAGTGAGTGAGCCCCGCCCGCGCCGTCCAGCGCAGCCTCGATCCCGAATACAttGCTGTACGCTTACCGGGGTTGTCTGGCCCGGGTCAGGACTGTGCGGCGCCCCCACATCCGGGTCCCTCTTCCGCTGACCCCCCCCCACATCCGGGTTCTGCCCCTCCCCCCAGGGGCCCCAGGACCCCGGCTCAAAACCCCGAGCCCTCGCACCCGGGGCCCAGACGTGGCGACCCCCAGGGGCAGCTCTTGGAGCTCGAGACATCGGTCCTACCAGGCCTTATCGGTCAGATGACCCCGCAGTCCCGGTCACTTCAGGCCTCAGTTTCCCCACCTGCTGCTTGACAACTGTCCCGGAGTTTTGGAGCCCGTCTTGATAGTTTCTGGTCTCGGGTTCAGGCTTGGGCCCTTTAGGTCGGGCAGTGAATCCCTCTACCCTTCTCTTTCTCCTGAAGCCCGGCTCCTGGGGCCGGCCCAGAAGCTAGCCAACTACGCGGGAGGCCCCGAAGCGCCGCTTGGGTCGCGTCTCCCGCAGGTCCTCTCTTCCGGGGCTGCCTCCTgcggccgc
Vector Type
Other
Antibiotic
Amp
Comments
CRISPR donor plasmid. Designed to KI an HA tag to the N-terminus of RAB1B when co-transfected with guides 48938 and 48952. Donor sequence shown as sequence of insert. Positives may be enriched for with puromycin prior to further selection by western blotting and FACs. Note this system does not require induction with Tet/Dox. May be used in any cell-line, contains several silent mutations to prevent binding of guides.
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