RAB1A

Synonyms
RAB1, YPT1
Plasmid
pMK-RQ RAB1A Nter HA donor
Parent Plasmid
pMK-RQ
DU Number
DU48990
Genbank
NM_004161.4
Species
Human
Sequence of Insert
gcggCCGCCAGGGGGAGTCCGAGCGAAGTCCTAGCCAGCGAGTCAGAGGGGAGGGGAGCAGGGAGGGGCCGAGGGTGGGGAGGTGAGGGAGTGGGGAATGGGGCGGGCGACAACCCTTCAGGTACGCATGCCCCAGAGGCGCGGCGCTTGGCGGGAAGCTGAGTCCTGGCCTTGCGTCGCACTGTCTGTCCTCAGCTCGCGTAGCCGCGCTCGCGACTCCCTTTCCCGGCATGCCAGGCGGTGCGGCCGCCCTCTGGGCCGTGTAAAGGCCCCTCGGTCTAAGGCTTCCCTATTTCCTGGTTCGCCGGCGGCCATTTTGGGTGGAAGCGATAGCTGAGTGGCGGCGGCTGCTGATTGTGTTCTAGGGGACGGAGTAGGGGAAGACGTTTGCTCTCCCGGAACAGCCTATCTCATTCCTTTCTTTCGATTACCCGTGGCGCGGAGAGTCAGGGCGGCGGCTGCGGCAGCAAGGGCGGCGGTGGCGGCGGCGGCAGCTGCAGTGACATGTACCCATACGATGTGCCAGATTACGCCagtAGCATGAATCCCGAATAGTGAGTTCAGGAGAGCACCaaTCGGCTGGGTCCGTGGGCCAGCTTGGGGGATCTTAAAGGGGTCGAGGAGGGTTGGGGCAGAAGTCGGGGCATCGGCTGGGGTGAGGCGAGGGTGATGGGTCAGGAGAGGCTGGCGGCCGGGAGTCGGGCCCCATTGTCTGACGCGGAGGGGCGGCCGCGCGGGGGAGGGGTCGGGCCGGAGGGGTGAGCCGCCCGGGCCTGGACCGGGTCAGGTTAGAGGGCCTGACTGCGGGGCGGGTGCTGAGGAAGCCTGCCGAGGGGCCTGGGGCGGTGTGAAGGGGTATCTTCTCTCGGAGGCAGTGACTTTTGAAGGAGGACTTGTCTCTAAGGGGAGGGGATGGGGTGGGAGAGCCCTTCTAGAGGGCACTGTCAGACCCTGCGCCCGCACTCTGCGGAGCTGTCAGGATCTTCGGGGTAGAAACCAGCTTTACTTGTAAATCCTGAGCTTGTTGGCTCTCTCTCCTTCCATCCTCCCCGCCAGGTTTCAGGTAATATGGATGCggccgc
Vector Type
Mammalian
Antibiotic
Kan
Comments
CRISPR donor plasmid. Designed to KI an HA tag to the N-terminus of RAB1A when co-transfected with guides 48937 and 48951. Donor sequence shown as sequence of insert. Positives may be enriched for with puromycin prior to further selection by western blotting and FACs. Note this system does not require induction with Tet/Dox. May be used in any cell-line, contains several silent mutations to prevent binding of guides.
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