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Synonyms
GIG4
Plasmid
pMK-RQ RAB13 Nter HA donor
Parent Plasmid
pMK-RQ
Expressed
RAB13 Nter KI sense guide
DU Number
DU48972
Genbank
NM_005370.4
Species
Human
Sequence of Insert
View Sequence
gcggccgCTCCAAAACTCAGCCCCATTTTTTTTTTTCAATTTCTAGTGCTCTCCCTACCTTCACCCTTATTCCCAAAACTCTTGGTTCTCTTCCCCTAATCCCTCTAAAATTTCTCTTCTAAAATTTCTGGTCGTCGTGGTTAAACCTCTACTTCCCTTCACCCCAGCCTCTCTGGCCTCAGAATGGAAGAGTAAGGTCCTAAGAGGGGAGCTACAATTACCGAAAGGCTGTGGAAAATAGCGAGAGAGACAGGCCGGCCTAAGCTAGGAAGGGAAAACCGAGAGAACAGAGGCCGGGCTGGCAGCCTTGGCCTGCTCAAAAGGGTGGGCGGGCAGGGGGTGGAGCCCGGAGGGGTGCCGCCTCTCCTCTTTCCCGGAGCCTGGGCGGAGAGGGAGGAAAACTTCTTCCTGGCCTGGGCTCCGTGCCGCTCTGTTTGCCAACCGTCCAGTCCCGCCTACCAGTGCCGGGCGCTCCCCACCCCTCCCCCGGCTCCCCCGGTGTCCGCCATGTACCCATACGATGTGCCAGATTACGCCGCgAAgGCaTACGAtCAtCTCTTCAAGctcCTGttaATaGGaGACTCGGGGGTGGGCAAGACTTGTCTGATCATTCGCTTTGCAGAGGACAACTTCAACAACACTTACATCTCCACCATCGGTGAGCCCGCTGGCCATGTCCCAGACCCCGACTCTCCTGTGACCTCCTTCTCCGGCTCCCGGATTACTAGTGACCCCACTTTTCAGTCCCCTTAGAGTAAGGCTCCTAAATTCTAGTCCCTCAACCCCTGGCTCTGACCCTTCCCCCGTGCATGTCTCTGTATAGTAACCAAACCTCTCACCTCTCTGAGGCCTTCAGGTAACTCTTCAACTGCTGCCAGCTCCTCATTTTCTTGGTACCATCGCCTTAGTCTCCCCAAATCCACAAGAACTCTTCTCTATTTTTCTCTGTTCCCCTCTTCCCCTACCCCCACCACCTAGAACTCTCTCGATCCTATCTCCATGTTTCCTGTATCCCTGTCCGCCACATCTTTTTCTCCCGATCCGATGCTAGCCTCTTTCTGTCTTGCTGAGCTTCAAACgcggccgc
Vector Type
Other
Antibiotic
Kan
Comments
CRISPR donor plasmid. Targets N-terminus of coding region, designed to KI an HA tag to the N-terminus of RAB13 when co-transfected with sense guide 48936 and antisense guide 48950. Donor sequence shown as sequence of insert, contains silent changes to prevent attack from Cas9. Positives may be enriched for with puromycin prior to further selection by western blotting and FACs. Note this system does not require induction with Tet/Dox. May be used in any cell-line though an empty TRex line is preferable as this allows for downstream rescue and mutant analysis if necessary.
100042 R 1000 1001 1047 19007
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