RAB8B

Plasmid
pX335 RAB8B Nter KI antisense
Parent Plasmid
pX335
Expressed
RAB8B Nter KI antisense guide + Cas9 D10A
DU Number
DU48949
Genbank
NM_016530.2
Species
Human
Sequence of Insert
gTCTTCGCCATCTTCTCGCTC
Vector Type
Mammalian
Antibiotic
Amp
Comments
CRISPR guide plasmid. Targets antisense strand of exon 1, designed to KI an HA to tag to the N-terminus of RAB8B when co-transfected with sense guide 48935 and donor 48994. Guide sequence shown as sequence of insert. Positives may be enriched for with puromycin prior to further selection by western blotting and FACs. Note this system does not require induction with Tet/Dox. May be used in any cell-line though an empty TRex line is preferable as this allows for downstream rescue and mutant analysis if necessary.
100042 R 1000 1001 1047 19007
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