pX335 RAB8A Nter KI antisense
RAB8A Nter KI antisense guide + Cas9 D10A
Sequence of InsertView Sequence
CRISPR guide plasmid. Targets antisense strand of exon 1, designed to KI an HA tag to the N-terminus of RAB8A when co-transfected with sense guide 48934 and donor 48993. Guide sequence shown as sequence of insert. Positives may be enriched for with puromycin prior to further selection by western blotting and FACs. Note this system does not require induction with Tet/Dox. May be used in any cell-line though an empty TRex line is preferable as this allows for downstream rescue and mutant analysis if necessary.
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