RAB1A

Synonyms
RAB1, YPT1
Plasmid
pBABED P U6 RAB1A Nter KI sense
Parent Plasmid
pBABED P U6
Expressed
RAB1A Nter KI sense
DU Number
DU48937
Genbank
NM_004161.4
Species
Human
Sequence of Insert
gTAGTGAGTTCAGGAGAGCAC

Vector Type
Mammalian
Antibiotic
Amp
Comments
CRISPR guide plasmid. Targets sense strand of exon 1, designed to KI an HA tag to the N-terminus of RAB1A when co-transfected with antisense guide 48951 and donor 48990. Guide sequence shown as sequence of insert. Positives may be enriched for with puromycin prior to further selection by western blotting and FACs. Note this system does not require induction with Tet/Dox. May be used in any cell-line though an empty TRex line is preferable as this allows for downstream rescue and mutant analysis if necessary.
100042 R 1000 1001 1047 19007
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