RAB13

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Synonyms
GIG4
Plasmid
pBABED P U6 RAB13 Nter KI sense
Parent Plasmid
pBABED P U6
Expressed
RAB13 Nter KI sense guide
DU Number
DU48936
Genbank
NM_005370.4
Species
Human
Sequence of Insert
gCTTCAAGTTGCTGCTGATCG
Vector Type
Mammalian
Antibiotic
Amp
Comments
CRISPR guide plasmid. Targets sense strand of exon 1, designed to KI an HA tag to the N-terminus of RAB13 when co-transfected with antisense guide 48950 and donor 48972. Guide sequence shown as sequence of insert. Positives may be enriched for with puromycin prior to further selection by western blotting and FACs. Note this system does not require induction with Tet/Dox. May be used in any cell-line though an empty TRex line is preferable as this allows for downstream rescue and mutant analysis if necessary.
100042 R 1000 1001 1047 19007
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