RAB8A

Synonyms
MEL, RAB8
Plasmid
pBABED P U6 RAB8A Nter KI sense
Parent Plasmid
pBABED P U6
Expressed
RAB8A Nter KI sense guide
DU Number
DU48934
Genbank
NM_005370.4
Species
Human
Sequence of Insert
gTGATCGGGGACTCGGGGGTG
Vector Type
Mammalian
Antibiotic
Amp
Comments
CRISPR guide plasmid. Targets sense strand of exon 1, designed to KI an HA tag to the N-terminus of RAB8A when co-transfected with antisense guide 48948 and donor 48993. Guide sequence shown as sequence of insert. Positives may be enriched for with puromycin prior to further selection by western blotting and FACs. Note this system does not require induction with Tet/Dox. May be used in any cell-line though an empty TRex line is preferable as this allows for downstream rescue and mutant analysis if necessary.
100042 R 1000 1001 1047 19007
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