Parkin | LRRK2 Resource

Plasmid

pLVX puro HA Parkin (mouse) S65A

Parent Plasmid

pLVX puro HA

Expressed

HA Parkin (mouse) S65A

DU Number

DU51093

Genbank

NM_016694.3

Tag

HA

Species

Mouse

Sequence of Insert

View Sequence

AGATCTATGATAGTGTTTGTCAGGTTCAACTCCAGCTATGGCTTCCCAGTGGAGGTCGATTCTGACACCAGCATCTTGCAGCTCAAGGAAGTGGTTGCTA
AGCGACAGGGGGTTCCAGCTGACCAGCTGCGTGTGATTTTTGCCGGGAAGGAGCTTCCGAATCACCTGACGGTTCAAAACTGCGACCTGGAACAACAGGC
TATTGTACACATAGTACAGAGACCACGGAGGAGAAGTCATGAAACAAATGCATCTGGAGGGGACGAACCCCAGAGCACCTCAGAGGGCTCCATATGGGAG
TCCAGGAGCTTGACACGAGTGGACCTGAGCAGCCATACCCTGCCGGTGGACTCTGTGGGGCTGGCGGTCATTCTGGACACAGACAGTAAGAGGGATTCAG
AAGCAGCCAGAGGTCCAGTTAAACCCACCTACAACAGCTTTTTCATCTACTGCAAAGGCCCCTGCCACAAGGTCCAGCCTGGAAAGCTCCGAGTTCAGTG
TGGCACCTGCAAACAAGCAACCCTCACCTTGGCCCAGGGCCCATCTTGCTGGGACGATGTCTTAATTCCAAACCGGATGAGTGGTGAGTGCCAGTCTCCA
GACTGCCCTGGAACCAGAGCTGAATTTTTCTTTAAATGTGGAGCACACCCAACCTCAGACAAGGACACGTCGGTAGCTTTGAACCTGATCACCAGCAACA
GGCGCAGCATCCCTTGCATAGCGTGCACAGATGTCAGGAGCCCTGTCCTGGTCTTCCAGTGTAACCACCGTCACGTGATCTGTTTGGACTGTTTCCACTT
GTATTGTGTCACAAGACTCAACGATCGGCAGTTTGTCCACGATGCTCAACTTGGCTACTCCCTGCCGTGTGTAGCTGGCTGTCCCAACTCCCTGATTAAA
GAGCTCCATCACTTCAGGATCCTTGGAGAAGAGCAGTACACTAGGTACCAGCAGTATGGGGCCGAGGAATGCGTGCTGCAAATGGGAGGTGTGCTGTGCC
CCCGTCCTGGCTGTGGAGCTGGACTGCTACCTGAACAGGGCCAGAGGAAAGTCACCTGCGAAGGGGGCAACGGCCTGGGCTGCGGGTTTGTTTTCTGCCG
GGACTGTAAGGAAGCATACCATGAAGGGGATTGCGACTCACTGCTCGAACCCTCAGGAGCCACTTCTCAGGCCTACAGGGTGGACAAAAGAGCCGCTGAG
CAAGCTCGCTGGGAGGAGGCCTCCAAGGAAACCATCAAGAAGACCACCAAGCCTTGTCCTCGCTGCAACGTGCCAATTGAAAAAAACGGAGGATGTATGC
ACATGAAGTGTCCTCAGCCCCAGTGCAAGCTGGAGTGGTGCTGGAACTGTGGCTGTGAGTGGAACCGAGCCTGCATGGGAGATCACTGGTTTGACGTGTA
GGCGGCCGC

Amino Acid Sequence

View Sequence

MYPYDVPDYAGSMIVFVRFNSSYGFPVEVDSDTSILQLKEVVAKRQGVPADQLRVIFAGKELPNHLTVQNCDLEQQAIVHIVQRPRRRSHETNASGGDEP
QSTSEGSIWESRSLTRVDLSSHTLPVDSVGLAVILDTDSKRDSEAARGPVKPTYNSFFIYCKGPCHKVQPGKLRVQCGTCKQATLTLAQGPSCWDDVLIP
NRMSGECQSPDCPGTRAEFFFKCGAHPTSDKDTSVALNLITSNRRSIPCIACTDVRSPVLVFQCNHRHVICLDCFHLYCVTRLNDRQFVHDAQLGYSLPC
VAGCPNSLIKELHHFRILGEEQYTRYQQYGAEECVLQMGGVLCPRPGCGAGLLPEQGQRKVTCEGGNGLGCGFVFCRDCKEAYHEGDCDSLLEPSGATSQ
AYRVDKRAAEQARWEEASKETIKKTTKPCPRCNVPIEKNGGCMHMKCPQPQCKLEWCWNCGCEWNRACMGDHWFDV*

Vector Type

Mammalian

Antibiotic

Amp

Comments

Please clone HA-tagged Parkin (mouse, full length) with a point mutation of Ser65 to Ala, into a lentiviral vector suitable for mammalian transfecton. Many thanks in advance.

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