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Parkin (tribolium)

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Plasmid
pET156P SUMO Parkin (tribolium)
Parent Plasmid
pET15b 6His SUMO1
Expressed
6HIS SUMO parkin (tribolium)
DU Number
DU46750
Genbank
XP_969040.2
Tag
6His
SUMO
Species
Tribolium castaneum
Sequence of Insert
GGATCCATGATGAGCTTTATCATCAACGTGATTAAAGATCTGCTGCATAAAATGCTGCAGTTTCTGAGCTTTAGCCGTAAAACCCTGAGCAATAATCTGA
GCATCTACATCAAAACCAATACCGGTAATACCCTGAGTGTTGATCTGGACCCGACCTGGGATATTAAAGAAGTGAAAGAATTTGTTGCACCGCAGCTGGG
TCTGAAACCGGGTGAAGTTAAAATCATTTTTGCCGGTAAAGAACTGGGCGATAACATTAGCATTAGCGAATGTGATCTGGGTCAGCAGAGCACCCTGCAT
GCAATCAAAATTACCAAAAAACCGAATCCGCCTACCCTGAATAATGCCGTTATTGAAGAAAGTGATGAAGGTAAACCGCTGTGTGAAACACTGAGCGATC
TGAGTTTTAGCAATGATGATAGCGAAGAAGGTCCGACCGAACAGCGTAAAGTTCATTTTTTTGTTTATTGCCCGACCTGCAAAGCCCTGAAAAATGGTAA
ACTGCGTGTTCGTTGCCACTTTTGTAAAAGCGGTGCATTTACCGTTCATAGCGATCCGCAGAATTGGAATGATGTTCTGCAGAGCAAACAAATTACCGGT
CTGTGCGAAAATAATCCGGAACTGTGTGCAAATGTGCTGAATAACAAAGAACCGACCTTTGCCGAGTTCTATTTCAAATGTAGCGAACATACCAGCCTGG
GTGAAACCGATGAAGCAGTTCCGCTGTATCTGATTCGTCCGAATCTGAAAGAGGTTCCTTGTCTGGCATGTGCCGATATTAATGAAACCGTTTTTGTGTT
TCCGTGCAAAGAAGGTCACGTTACCTGTCTGGATTGTTTTCGTCAGTATTGTACCACCCGTCTGCGTGAACGTCAGTTTTGGCAGCATCCGGAATTTGGT
TATACCCTGGCATGTCCGGCAGGTTGTCCGGATAGCTTTATTCAAGAAATTCATCATTTTCGCCTGCTGAGCGAAAGCCAGTATACCCAGTATCAGCGTT
TTGCAACCGAAGAATATGTGCTGCGTAGCGGTGGTGTTCTGTGTCCGCAGCCTGGTTGTGGTATGGGTATTCTGGCCGATCCGGAATGTACCAAAATCAC
CTGTATTAATGGTTGCGGCTATGTGTTTTGTCGTCTGTGTCTGCAGGGTTTTCATATTGGTGAATGTGAAGCACCGGAAGGTGATGTTGGTGCACCGGGT
GATTGTAGCTATAATGTTGATCCGACCCGTGCAGCCGAAGCACGTTGGGATGAAGCAAGCAAAGTTGCAATTAAAGTTCTGACCAAACCGTGTCCGAAAT
GTCGTACCCCGACAGAACGTGATGGTTAAGC
Amino Acid Sequence
MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTHLKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGMMSFIINVIKDLLHKMLQFLSFSRKTLSNNLSIYIKTNTGNTLSVDLDPTWDIKEVKEFVAPQLGLKPGEVKIIFAGKELGDNISISECDLGQQST
LHAIKITKKPNPPTLNNAVIEESDEGKPLCETLSDLSFSNDDSEEGPTEQRKVHFFVYCPTCKALKNGKLRVRCHFCKSGAFTVHSDPQNWNDVLQSKQI
TGLCENNPELCANVLNNKEPTFAEFYFKCSEHTSLGETDEAVPLYLIRPNLKEVPCLACADINETVFVFPCKEGHVTCLDCFRQYCTTRLRERQFWQHPE
FGYTLACPAGCPDSFIQEIHHFRLLSESQYTQYQRFATEEYVLRSGGVLCPQPGCGMGILADPECTKITCINGCGYVFCRLCLQGFHIGECEAPEGDVGA
PGDCSYNVDPTRAAEARWDEASKVAIKVLTKPCPKCRTPTERDG*
Vector Type
Bacterial
Antibiotic
Amp
Comments
codon optimised for E coli expression
100042 R 1000 1001 1047 19007
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