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Parkin (mouse)

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Plasmid
pcDNA5 FRT/TO Parkin (mouse) C430F
Parent Plasmid
pcDNA5 FRT/TO
Expressed
Parkin C430F (mouse)
DU Number
DU46691
Genbank
NM_016694.3
Species
Mouse
Sequence of Insert
GAATTCGCCCTTCGGATCCGCCACCATGATAGTGTTTGTCAGGTTCAACT
CCAGCTATGGCTTCCCAGTGGAGGTCGATTCTGACACCAGCATCTTGCAG
CTCAAGGAAGTGGTTGCTAAGCGACAGGGGGTTCCAGCTGACCAGCTGCG
TGTGATTTTTGCCGGGAAGGAGCTTCCGAATCACCTGACGGTTCAAAACT
GTGACCTGGAACAACAGAGTATTGTACACATAGTACAGAGACCACGGAGG
AGAAGTCATGAAACAAATGCATCTGGAGGGGACGAACCCCAGAGCACCTC
AGAGGGCTCCATATGGGAGTCCAGGAGCTTGACACGAGTGGACCTGAGCA
GCCATACCCTGCCGGTGGACTCTGTGGGGCTGGCGGTCATTCTGGACACA
GACAGTAAGAGGGATTCAGAAGCAGCCAGAGGTCCAGTTAAACCCACCTA
CAACAGCTTTTTCATCTACTGCAAAGGCCCCTGCCACAAGGTCCAGCCTG
GAAAGCTCCGAGTTCAGTGTGGCACCTGCAAACAAGCAACCCTCACCTTG
GCCCAGGGCCCATCTTGCTGGGACGATGTCTTAATTCCAAACCGGATGAG
TGGTGAGTGCCAGTCTCCAGACTGCCCTGGAACCAGAGCTGAATTTTTCT
TTAAATGTGGAGCACACCCAACCTCAGACAAGGACACGTCGGTAGCTTTG
AACCTGATCACCAGCAACAGGCGCAGCATCCCTTGCATAGCGTGCACAGA
TGTCAGGAGCCCTGTCCTGGTCTTCCAGTGTAACCACCGTCACGTGATCT
GTTTGGACTGTTTCCACTTGTATTGTGTCACAAGACTCAACGATCGGCAG
TTTGTCCACGATGCTCAACTTGGCTACTCCCTGCCGTGTGTAGCTGGCTG
TCCCAACTCCCTGATTAAAGAGCTCCATCACTTCAGGATCCTTGGAGAAG
AGCAGTACACTAGGTACCAGCAGTATGGGGCCGAGGAATGCGTGCTGCAA
ATGGGAGGTGTGCTGTGCCCCCGTCCTGGCTGTGGAGCTGGACTGCTACC
TGAACAGGGCCAGAGGAAAGTCACCTGCGAAGGGGGCAACGGCCTGGGCT
GCGGGTTTGTTTTCTGCCGGGACTGTAAGGAAGCATACCATGAAGGGGAT
TGCGACTCACTGCTCGAACCCTCAGGAGCCACTTCTCAGGCCTACAGGGT
GGACAAAAGAGCCGCTGAGCAAGCTCGCTGGGAGGAGGCCTCCAAGGAAA
CCATCAAGAAGACCACCAAGCCTTGTCCTCGCTGCAACGTGCCAATTGAA
AAAAACGGAGGATTTATGCACATGAAGTGTCCTCAGCCCCAGTGCAAGCT
GGAGTGGTGCTGGAACTGTGGCTGTGAGTGGAACCGAGCCTGCATGGGAG
ATCACTGGTTTGACGTGTAGGCGGCCGCCCAAGGGCGAATTC
Amino Acid Sequence
MIVFVRFNSSYGFPVEVDSDTSILQLKEVVAKRQGVPADQLRVIFAGKEL
PNHLTVQNCDLEQQSIVHIVQRPRRRSHETNASGGDEPQSTSEGSIWESR
SLTRVDLSSHTLPVDSVGLAVILDTDSKRDSEAARGPVKPTYNSFFIYCK
GPCHKVQPGKLRVQCGTCKQATLTLAQGPSCWDDVLIPNRMSGECQSPDC
PGTRAEFFFKCGAHPTSDKDTSVALNLITSNRRSIPCIACTDVRSPVLVF
QCNHRHVICLDCFHLYCVTRLNDRQFVHDAQLGYSLPCVAGCPNSLIKEL
HHFRILGEEQYTRYQQYGAEECVLQMGGVLCPRPGCGAGLLPEQGQRKVT
CEGGNGLGCGFVFCRDCKEAYHEGDCDSLLEPSGATSQAYRVDKRAAEQA
RWEEASKETIKKTTKPCPRCNVPIEKNGGFMHMKCPQPQCKLEWCWNCGC
EWNRACMGDHWFDV*
Vector Type
Mammalian
Antibiotic
Amp
Comments
Please insert a point mutation at C430 to F, in wild-type mouse Parkin. Please construct in an FRT-TO vector. Many thanks in advance.
100042 R 1000 1001 1047 19007
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