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Plasmid
pMAL4C Rab13 S164A S173A S178A T191A
Parent Plasmid
pMAL4C
Expressed
MBP Rab13 S164A S173A S178A T191A
DU Number
DU46480
Genbank
Q504R6
Tag
MBP
Species
Human
Sequence of Insert
View Sequence
GGATCCATGGCCAAAGCCTACGACCACCTCTTCAAGTTGCTGCTGATCGGGGACTCGGGGGTGGGCAAGACTTGTCTGATCATTCGCTTTGCAGAGGACA
ACTTCAACAACACTTACATCTCCACCATCGGAATTGATTTCAAGATCCGCACTGTGGATATAGAGGGGAAGAAGATCAAACTACAAGTCTGGGACACGGC
TGGCCAAGAGCGGTTCAAGACAATAACTACTGCCTACTACCGTGGAGCCATGGGCATTATCCTAGTATACGACATCACGGATGAGAAATCTTTCGAGAAT
ATTCAGAACTGGATGAAAAGCATCAAGGAGAATGCCTCGGCTGGGGTGGAGCGCCTCTTGCTGGGGAACAAATGTGACATGGAGGCCAAGAGGAAGGTGC
AGAAGGAGCAGGCCGATAAGTTGGCTCGAGAGCATGGAATCCGATTTTTCGAAACTAGTGCTAAATCCAGTATGAATGTGGATGAGGCTTTTAGTGCCCT
GGCCCGGGACATCTTGCTCAAGGCAGGAGGCCGGAGAGCAGGAAACGGCAACAAGCCTCCCAGTACTGACCTGAAAGCTTGTGACAAGAAGAACACCAAC
AAGTGCTCCCTGGGCTGAGCGGCCGC
ACTTCAACAACACTTACATCTCCACCATCGGAATTGATTTCAAGATCCGCACTGTGGATATAGAGGGGAAGAAGATCAAACTACAAGTCTGGGACACGGC
TGGCCAAGAGCGGTTCAAGACAATAACTACTGCCTACTACCGTGGAGCCATGGGCATTATCCTAGTATACGACATCACGGATGAGAAATCTTTCGAGAAT
ATTCAGAACTGGATGAAAAGCATCAAGGAGAATGCCTCGGCTGGGGTGGAGCGCCTCTTGCTGGGGAACAAATGTGACATGGAGGCCAAGAGGAAGGTGC
AGAAGGAGCAGGCCGATAAGTTGGCTCGAGAGCATGGAATCCGATTTTTCGAAACTAGTGCTAAATCCAGTATGAATGTGGATGAGGCTTTTAGTGCCCT
GGCCCGGGACATCTTGCTCAAGGCAGGAGGCCGGAGAGCAGGAAACGGCAACAAGCCTCCCAGTACTGACCTGAAAGCTTGTGACAAGAAGAACACCAAC
AAGTGCTCCCTGGGCTGAGCGGCCGC
Amino Acid Sequence
View Sequence
MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRY
NGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLI
KNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPL
GAVALKSYEEELVKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTNSSSNNNNNNNNNNLGDDDDKVPEFLEVLFQGP
GSMAKAYDHLFKLLLIGDSGVGKTCLIIRFAEDNFNNTYISTIGIDFKIRTVDIEGKKIKLQVWDTAGQERFKTITTAYYRGAMGIILVYDITDEKSFEN
IQNWMKSIKENASAGVERLLLGNKCDMEAKRKVQKEQADKLAREHGIRFFETSAKSSMNVDEAFSALARDILLKAGGRRAGNGNKPPSTDLKACDKKNTN
KCSLG*
NGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLI
KNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPL
GAVALKSYEEELVKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTNSSSNNNNNNNNNNLGDDDDKVPEFLEVLFQGP
GSMAKAYDHLFKLLLIGDSGVGKTCLIIRFAEDNFNNTYISTIGIDFKIRTVDIEGKKIKLQVWDTAGQERFKTITTAYYRGAMGIILVYDITDEKSFEN
IQNWMKSIKENASAGVERLLLGNKCDMEAKRKVQKEQADKLAREHGIRFFETSAKSSMNVDEAFSALARDILLKAGGRRAGNGNKPPSTDLKACDKKNTN
KCSLG*
Vector Type
Bacterial
Antibiotic
Amp
Comments
mutate wild-type clone DU43886; small scale pilot expression please
100042 R 1000 1001 1047 19007
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