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Plasmid
pGEX6P-1 parkin 1-76 TAG amber
Parent Plasmid
pGEX6P1
Expressed
GST-Parkin [residues 1-76] [mutate Ser65 codon to TAG]
DU Number
DU43028
Genbank
O60260
Tag
GST
Species
Human
Sequence of Insert
View Sequence
GGATCCATGATAGTGTTTGTCAGGTTCAACTCCAGCCATGGTTTCCCAGT
GGAGGTCGATTCTGACACCAGCATCTTCCAGCTCAAGGAGGTGGTTGCTA
AGCGACAGGGGGTTCCGGCTGACCAGTTGCGTGTGATTTTCGCAGGGAAG
GAGCTGAGGAATGACTGGACTGTGCAGAATTGTGACCTGGATCAGCAGAG
CATTGTTCACATTGTGCAGAGACCGTGGAGAAAATagGCGGCCGC
GGAGGTCGATTCTGACACCAGCATCTTCCAGCTCAAGGAGGTGGTTGCTA
AGCGACAGGGGGTTCCGGCTGACCAGTTGCGTGTGATTTTCGCAGGGAAG
GAGCTGAGGAATGACTGGACTGTGCAGAATTGTGACCTGGATCAGCAGAG
CATTGTTCACATTGTGCAGAGACCGTGGAGAAAATagGCGGCCGC
Amino Acid Sequence
View Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMIVFVRFNSSHGFPVEVDSDTSIFQLKEVVAKRQGVPADQLRVIFAGKELRNDWTVQNCDLDQQSIVHIVQRPWRK*
Vector Type
Bacterial
Antibiotic
Amp
Comments
Dear Rachel - as discussed, please use clone DU37369 and mutate Ser65 codon to an Amber TAG stop codon. Urgent please.
100042 R 1000 1001 1047 19007
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