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parkin

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Plasmid
pET6HIS SUMO Parkin Q316A
Parent Plasmid
pET15b 6His SUMO1
Expressed
His-SUMO-Parkin [Q316A]
DU Number
DU46037
Genbank
O60260
Tag
SUMO
6His
Species
Human
Sequence of Insert
CCATGGGTCATCATCACCATCACCATTCTGACCAGGAGGCAAAACCTTCA
ACTGAGGACTTGGGGGATAAGAAGGAAGGTGAATATATTAAACTCAAAGT
CATTGGACAGGATAGCAGTGAGATTCACTTCAAAGTGAAAATGACAACAC
ATCTCAAGAAACTCAAAGAATCATACTGTCAAAGACAGGGTGTTCCAATG
AACTCACTCAGGTTTCTCTTTGAGGGTCAGAGAATTGCTGATAATCATAC
TCCAAAAGAACTGGGAATGGAGGAAGAAGATGTGATTGAAGTTTATCAGG
AACAAACGGGGGGAatgatagtgtttgtcaggttcaactccagccatggt
ttcccagtggaggtcgattctgacaccagcatcttccagctcaaggaggt
ggttgctaagcgacagggggttccggctgaccagttgcgtgtgattttcg
cagggaaggagctgaggaatgactggactgtgcagaattgtgacctggat
cagcagagcattgttcacattgtgcagagaccgtggagaaaaggtcaaga
aatgaatgcaactggaggcgacgaccccagaaacgcggcgggaggctgtg
agcgggagccccagagcttgactcgggtggacctcagcagctcagtcctc
ccaggagactctgtggggctggctgtcattctgcacactgacagcaggaa
ggactcaccaccagctggaagtccagcaggtagatcaatctacaacagct
tttatgtgtattgcaaaggcccctgtcaaagagtgcagccgggaaaactc
agggtacagtgcagcacctgcaggcaggcaacgctcaccttgacccaggg
tccatcttgctgggatgatgttttaattccaaaccggatgagtggtgaat
gccaatccccacactgccctgggactagtgcagaatttttctttaaatgt
ggagcacaccccacctctgacaaggaaacatcagtagctttgcacctgat
cgcaacaaatagtcggaacatcacttgcattacgtgcacagacgtcagga
gccccgtcctggttttccagtgcaactcccgccacgtgatttgcttagac
tgtttccacttatactgtgtgacaagactcaatgatcggcagtttgttca
cgaccctcaacttggctactccctgccttgtgtggctggctgtcccaact
ccttgattaaagagctccatcacttcaggattctgggagaagagcagtac
aaccggtacGCgcagtatggtgcagaggagtgtgtcctgcagatgggggg
cgtgttatgcccccgccctggctgtggagcggggctgctgccggagcctg
accagaggaaagtcacctgcgaagggggcaatggcctgggctgtgggttt
gccttctgccgggaatgtaaagaagcgtaccatgaaggggagtgcagtgc
cgtatttgaagcctcaggaacaactactcaggcctacagagtcgatgaaa
gagccgccgagcaggctcgttgggaagcagcctccaaagaaaccatcaag
aaaaccaccaagccctgtccccgctgccatgtaccagtggaaaaaaatgg
aggctgcatgcacatgaagtgtccgcagccccagtgcaggctcgagtggt
gctggaactgtggctgcgagtggaaccgcgtctgcatgggggaccactgg
ttcgacgtgtaggcggccgc
Amino Acid Sequence
MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTH
LKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGMIVFVRFNSSHGFPVEVDSDTSIFQLKEVVAKRQGVPADQLRVIFA
GKELRNDWTVQNCDLDQQSIVHIVQRPWRKGQEMNATGGDDPRNAAGGCE
REPQSLTRVDLSSSVLPGDSVGLAVILHTDSRKDSPPAGSPAGRSIYNSF
YVYCKGPCQRVQPGKLRVQCSTCRQATLTLTQGPSCWDDVLIPNRMSGEC
QSPHCPGTSAEFFFKCGAHPTSDKETSVALHLIATNSRNITCITCTDVRS
PVLVFQCNSRHVICLDCFHLYCVTRLNDRQFVHDPQLGYSLPCVAGCPNS
LIKELHHFRILGEEQYNRYAQYGAEECVLQMGGVLCPRPGCGAGLLPEPD
QRKVTCEGGNGLGCGFAFCRECKEAYHEGECSAVFEASGTTTQAYRVDER
AAEQARWEAASKETIKKTTKPCPRCHVPVEKNGGCMHMKCPQPQCRLEWC
WNCGCEWNRVCMGDHWFDV*
Vector Type
Bacterial
Antibiotic
Amp
Comments
in pET vector
100042 R 1000 1001 1047 19007
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