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Plasmid
pET6HIS SUMO PArkin 80-465 K161A
Parent Plasmid
pET15b 6His SUMO1
Expressed
His-SUMO-Parkin 80-465 K161A
DU Number
DU43843
Genbank
O60260
Tag
SUMO
6His
Amino Acid Sequence
View Sequence
MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTH
LKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGMNATGGDDPRNAAGGCEREPQSLTRVDLSSSVLPGDSVGLAVILHT
DSRKDSPPAGSPAGRSIYNSFYVYCKGPCQRVQPGALRVQCSTCRQATLT
LTQGPSCWDDVLIPNRMSGECQSPHCPGTSAEFFFKCGAHPTSDKETSVA
LHLIATNSRNITCITCTDVRSPVLVFQCNSRHVICLDCFHLYCVTRLNDR
QFVHDPQLGYSLPCVAGCPNSLIKELHHFRILGEEQYNRYQQYGAEECVL
QMGGVLCPRPGCGAGLLPEPDQRKVTCEGGNGLGCGFAFCRECKEAYHEG
ECSAVFEASGTTTQAYRVDERAAEQARWEAASKETIKKTTKPCPRCHVPV
EKNGGCMHMKCPQPQCRLEWCWNCGCEWNRVCMGDHWFDV*
LKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGMNATGGDDPRNAAGGCEREPQSLTRVDLSSSVLPGDSVGLAVILHT
DSRKDSPPAGSPAGRSIYNSFYVYCKGPCQRVQPGALRVQCSTCRQATLT
LTQGPSCWDDVLIPNRMSGECQSPHCPGTSAEFFFKCGAHPTSDKETSVA
LHLIATNSRNITCITCTDVRSPVLVFQCNSRHVICLDCFHLYCVTRLNDR
QFVHDPQLGYSLPCVAGCPNSLIKELHHFRILGEEQYNRYQQYGAEECVL
QMGGVLCPRPGCGAGLLPEPDQRKVTCEGGNGLGCGFAFCRECKEAYHEG
ECSAVFEASGTTTQAYRVDERAAEQARWEAASKETIKKTTKPCPRCHVPV
EKNGGCMHMKCPQPQCRLEWCWNCGCEWNRVCMGDHWFDV*
Vector Type
Bacterial
Antibiotic
Amp
Comments
Introduce K161A mutation into clone DU39813
100042 R 1000 1001 1047 19007
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