Parkin | LRRK2 Resource

Plasmid

pET6HIS SUMO PArkin K161A

Parent Plasmid

pET15b 6His SUMO1

Expressed

His-SUMO Parkin [K161A]

DU Number

DU43800

Genbank

O60260

Tag

SUMO

6His

Species

Human

Sequence of Insert

View Sequence

ATGGGTCATCATCACCATCACCATTCTGACCAGGAGGCAAAACCTTCAAC
TGAGGACTTGGGGGATAAGAAGGAAGGTGAATATATTAAACTCAAAGTCA
TTGGACAGGATAGCAGTGAGATTCACTTCAAAGTGAAAATGACAACACAT
CTCAAGAAACTCAAAGAATCATACTGTCAAAGACAGGGTGTTCCAATGAA
CTCACTCAGGTTTCTCTTTGAGGGTCAGAGAATTGCTGATAATCATACTC
CAAAAGAACTGGGAATGGAGGAAGAAGATGTGATTGAAGTTTATCAGGAA
CAAACGGGGGGAatgatagtgtttgtcaggttcaactccagccatggttt
cccagtggaggtcgattctgacaccagcatcttccagctcaaggaggtgg
ttgctaagcgacagggggttccggctgaccagttgcgtgtgattttcgca
gggaaggagctgaggaatgactggactgtgcagaattgtgacctggatca
gcagagcattgttcacattgtgcagagaccgtggagaaaaggtcaagaaa
tgaatgcaactggaggcgacgaccccagaaacgcggcgggaggctgtgag
cgggagccccagagcttgactcgggtggacctcagcagctcagtcctccc
aggagactctgtggggctggctgtcattctgcacactgacagcaggaagg
actcaccaccagctggaagtccagcaggtagatcaatctacaacagcttt
tatgtgtattgcaaaggcccctgtcaaagagtgcagccgggagcactcag
ggtacagtgcagcacctgcaggcaggcaacgctcaccttgacccagggtc
catcttgctgggatgatgttttaattccaaaccggatgagtggtgaatgc
caatccccacactgccctgggactagtgcagaatttttctttaaatgtgg
agcacaccccacctctgacaaggaaacatcagtagctttgcacctgatcg
caacaaatagtcggaacatcacttgcattacgtgcacagacgtcaggagc
cccgtcctggttttccagtgcaactcccgccacgtgatttgcttagactg
tttccacttatactgtgtgacaagactcaatgatcggcagtttgttcacg
accctcaacttggctactccctgccttgtgtggctggctgtcccaactcc
ttgattaaagagctccatcacttcaggattctgggagaagagcagtacaa
ccggtaccagcagtatggtgcagaggagtgtgtcctgcagatggggggcg
tgttatgcccccgccctggctgtggagcggggctgctgccggagcctgac
cagaggaaagtcacctgcgaagggggcaatggcctgggctgtgggtttgc
cttctgccgggaatgtaaagaagcgtaccatgaaggggagtgcagtgccg
tatttgaagcctcaggaacaactactcaggcctacagagtcgatgaaaga
gccgccgagcaggctcgttgggaagcagcctccaaagaaaccatcaagaa
aaccaccaagccctgtccccgctgccatgtaccagtggaaaaaaatggag
gctgcatgcacatgaagtgtccgcagccccagtgcaggctcgagtggtgc
tggaactgtggctgcgagtggaaccgcgtctgcatgggggaccactggtt
cgacgtgtaggcggccgc

Amino Acid Sequence

View Sequence

MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTH
LKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGMIVFVRFNSSHGFPVEVDSDTSIFQLKEVVAKRQGVPADQLRVIFA
GKELRNDWTVQNCDLDQQSIVHIVQRPWRKGQEMNATGGDDPRNAAGGCE
REPQSLTRVDLSSSVLPGDSVGLAVILHTDSRKDSPPAGSPAGRSIYNSF
YVYCKGPCQRVQPGALRVQCSTCRQATLTLTQGPSCWDDVLIPNRMSGEC
QSPHCPGTSAEFFFKCGAHPTSDKETSVALHLIATNSRNITCITCTDVRS
PVLVFQCNSRHVICLDCFHLYCVTRLNDRQFVHDPQLGYSLPCVAGCPNS
LIKELHHFRILGEEQYNRYQQYGAEECVLQMGGVLCPRPGCGAGLLPEPD
QRKVTCEGGNGLGCGFAFCRECKEAYHEGECSAVFEASGTTTQAYRVDER
AAEQARWEAASKETIKKTTKPCPRCHVPVEKNGGCMHMKCPQPQCRLEWC
WNCGCEWNRVCMGDHWFDV*

Vector Type

Bacterial

Antibiotic

Amp

Comments

in pET vector with no overhanging residues between His-SUMO and start of protein

100042 R 1000 1001 1047 19007