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Plasmid
pGEX6P-1 TEV CCPgCCgggg Parkin 1-76
Parent Plasmid
pGEX6P1
Expressed
GST-TEV-CCPGCCGGGG-Parkin Ubl domain (1-76)
DU Number
DU43676
Genbank
O60260
Tag
GST
Species
Human
Sequence of Insert
View Sequence
GGATCCGAAAACCTGTATTTTCAGTGTTGCCCTGGTTGTTGCGGTGGCGG
AGGGATGATAGTGTTTGTCAGGTTCAACTCCAGCCATGGTTTCCCAGTGG
AGGTCGATTCTGACACCAGCATCTTCCAGCTCAAGGAGGTGGTTGCTAAG
CGACAGGGGGTTCCGGCTGACCAGTTGCGTGTGATTTTCGCAGGGAAGGA
GCTGAGGAATGACTGGACTGTGCAGAATTGTGACCTGGATCAGCAGAGCA
TTGTTCACATTGTGCAGAGACCGTGGAGAAAATAAAGATCTCCAAGGGCG
AATTCCACATTGGTCGCTGCAGCCCGGGGGATCC
AGGGATGATAGTGTTTGTCAGGTTCAACTCCAGCCATGGTTTCCCAGTGG
AGGTCGATTCTGACACCAGCATCTTCCAGCTCAAGGAGGTGGTTGCTAAG
CGACAGGGGGTTCCGGCTGACCAGTTGCGTGTGATTTTCGCAGGGAAGGA
GCTGAGGAATGACTGGACTGTGCAGAATTGTGACCTGGATCAGCAGAGCA
TTGTTCACATTGTGCAGAGACCGTGGAGAAAATAAAGATCTCCAAGGGCG
AATTCCACATTGGTCGCTGCAGCCCGGGGGATCC
Amino Acid Sequence
View Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSENLYFQCCPGCCGGGGMIV
FVRFNSSHGFPVEVDSDTSIFQLKEVVAKRQGVPADQLRVIFAGKELRND
WTVQNCDLDQQSIVHIVQRPWRK*
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSENLYFQCCPGCCGGGGMIV
FVRFNSSHGFPVEVDSDTSIFQLKEVVAKRQGVPADQLRVIFAGKELRND
WTVQNCDLDQQSIVHIVQRPWRK*
Vector Type
Bacterial
Antibiotic
Amp
Comments
blunt TEV cleavage site so that no overhanging residues between the TEV site and CCPGCC (FlAsH tag) - pGEX6P-1 TEV vector
100042 R 1000 1001 1047 19007
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