This reagent and more can be found at our sister site MRC PPU Reagents & Services 
Request Now
Plasmid
pEBG-6 LRRK2 Kin Dom D1972/A dead
Parent Plasmid
pEBG6P
Expressed
GST-6-LRRK2-Kin Dom-D1972/A-dead 1856-2145
DU Number
DU6021
Genbank
XM_058513, LRRK2 Sugen
Tag
GST
Species
Human
Sequence of Insert
View Sequence
GGATCCGCCCCTGACTTGATTTTGGCTGACCTGCCTAGAAATATTATGTT
GAATAGTGATGAGTTGGAATTTGAACAAGCTCCAGAGTTTCTCCTAGGTG
ATGGCAGTTTTGGATCAGTTTACCGAGCAGCCTATGAAGGAGAAGAAGTG
GCTGTGAAGATTTTTAATAAACATACATCACTCAGGCTGTTAAGACAAGA
GCTTGTGGTGCTTTGCCACCTCCACCACCCCAGTTTGATATCTTTGCTGG
CAGCTGGGATTCGTCCCCGGATGTTGGTGATGGAGTTAGCCTCCAAGGGT
TCCTTGGATCGCCTGCTTCAGCAGGACAAAGCCAGCCTCACTAGAACCCT
ACAGCACAGGATCGCACTCCACGTAGCTGATGGTTTGAGATACCTCCACT
CAGCCATGATTATATACCGAGACCTGAAACCCCACAATGTGCTGCTTTTC
ACACTGTATCCCAATGCTGCCATCATCGCAAAGATTGCTGACTACGGCAT
TGCTCAGTACTGCTGTAGAATGGGGATAAAAACATCAGAGGGCACACCAG
GGTTTCGTGCACCTGAAGTTGCCAGAGGAAATGTCATTTATAACCAACAG
GCTGATGTTTATTCATTTGGTTTACTACTCTATGACATTTTGACAACTGG
AGGTAGAATAGTAGAGGGTTTGAAGTTTCCAAATGAGTTTGATGAATTAG
AAATACAAGGAAAATTACCTGATCCAGTTAAAGAATATGGTTGTGCCCCA
TGGCCTATGGTTGAAAGATTAATTAAACAGTGTTTGAAAGAAAATCCTCA
AGAAAGGCCTACTTCTGCCCAGGTCTTTGACATTTTGAATTCAGCTGAAT
TAGTCTGTCTGACGAGACGCATTTTATAGGATCC
GAATAGTGATGAGTTGGAATTTGAACAAGCTCCAGAGTTTCTCCTAGGTG
ATGGCAGTTTTGGATCAGTTTACCGAGCAGCCTATGAAGGAGAAGAAGTG
GCTGTGAAGATTTTTAATAAACATACATCACTCAGGCTGTTAAGACAAGA
GCTTGTGGTGCTTTGCCACCTCCACCACCCCAGTTTGATATCTTTGCTGG
CAGCTGGGATTCGTCCCCGGATGTTGGTGATGGAGTTAGCCTCCAAGGGT
TCCTTGGATCGCCTGCTTCAGCAGGACAAAGCCAGCCTCACTAGAACCCT
ACAGCACAGGATCGCACTCCACGTAGCTGATGGTTTGAGATACCTCCACT
CAGCCATGATTATATACCGAGACCTGAAACCCCACAATGTGCTGCTTTTC
ACACTGTATCCCAATGCTGCCATCATCGCAAAGATTGCTGACTACGGCAT
TGCTCAGTACTGCTGTAGAATGGGGATAAAAACATCAGAGGGCACACCAG
GGTTTCGTGCACCTGAAGTTGCCAGAGGAAATGTCATTTATAACCAACAG
GCTGATGTTTATTCATTTGGTTTACTACTCTATGACATTTTGACAACTGG
AGGTAGAATAGTAGAGGGTTTGAAGTTTCCAAATGAGTTTGATGAATTAG
AAATACAAGGAAAATTACCTGATCCAGTTAAAGAATATGGTTGTGCCCCA
TGGCCTATGGTTGAAAGATTAATTAAACAGTGTTTGAAAGAAAATCCTCA
AGAAAGGCCTACTTCTGCCCAGGTCTTTGACATTTTGAATTCAGCTGAAT
TAGTCTGTCTGACGAGACGCATTTTATAGGATCC
Amino Acid Sequence
View Sequence
MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSAPDLILADLPRNI
MLNSDELEFEQAPEFLLGDGSFGSVYRAAYEGEEVAVKIFNKHTSLRLLR
QELVVLCHLHHPSLISLLAAGIRPRMLVMELASKGSLDRLLQQDKASLTR
TLQHRIALHVADGLRYLHSAMIIYRDLKPHNVLLFTLYPNAAIIAKIAAY
GIAQYCCRMGIKTSEGTPGFRAPEVARGNVIYNQQADVYSFGLLLYDILT
TGGRIVEGLKFPNEFDELEIQGKLPDPVKEYGCAPWPMVERLIKQCLKEN
PQERPTSAQVFDILNSAELVCLTRRIL*
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSAPDLILADLPRNI
MLNSDELEFEQAPEFLLGDGSFGSVYRAAYEGEEVAVKIFNKHTSLRLLR
QELVVLCHLHHPSLISLLAAGIRPRMLVMELASKGSLDRLLQQDKASLTR
TLQHRIALHVADGLRYLHSAMIIYRDLKPHNVLLFTLYPNAAIIAKIAAY
GIAQYCCRMGIKTSEGTPGFRAPEVARGNVIYNQQADVYSFGLLLYDILT
TGGRIVEGLKFPNEFDELEIQGKLPDPVKEYGCAPWPMVERLIKQCLKEN
PQERPTSAQVFDILNSAELVCLTRRIL*
Vector Type
Mammalian
Antibiotic
Amp
Comments
All LRRK2 plasmids MUST be grown at 30°C or less to prevent recombination Contains SNP S1647T
100042 R 1000 1001 1047 19007
Download datasheet