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Plasmid
pPIC9K Pink1 150-end tribolium 6HIS (opt) D359A E527A K528A Delta I261-L270
Parent Plasmid
pPIC9K
Expressed
alpha factor signal sequence Pink1 150-end 6HIS D359A E527A K528A Delta I261-L270 (tribolium)
DU Number
DU56022
Species
Tribolium castaneum
Sequence of Insert
View Sequence
GAATTCTCCAGATTTGAGAGTAATCCGATTACCTTGAACGACTTGTCATTAGGCAAGCCAATTGCCAAAGGAACTAACGGTGTCGTATATTCAGCTAAAG
TCAAAGACGATGAAACCGATGATAACAAGTATCCCTTTGCTCTTAAGATGATGTTCAACTACGACATTCAGTCCAATAGCATGGAGATCTTGAAGGCCAT
GTACAGAGAAACTGTGCCAGCTAGAATGTATTACAGTAATCACGATTTGAACAATTGGGAGATAGAGTTGGCTAACAGAAGGAAACACCTTCCACCGCAT
CCAAACATAGTAGCCATTTTCTCCGTGTTTACCGACTTGTATCCAGCTGCTTTACCACCTAGGTTGCATCCTGAAGGAGAAGGACGAAATATGTCCTTGT
TCCTATTGATGAAGCGTTATGACTGCAATCTTCAAAGCTTCCTTTCTACGGCACCTTCAACAAGAACAAGTCTGTTGTTATTGGCACAACTGCTGGAAGG
AGTCGCACATATGACTGCTCACGGTATTGCTCATAGAGACTTGAAGTCGGACAATTTGTTGCTTGATACATCTGAACCTGAAAGTCCAATTCTGGTGATT
TCTGCTTTTGGCTGTTGTTTGGCAGATAAAACCAATGGGTTATCCTTGCCCTATACCTCGTACGAAATGGATAAAGGTGGGAACACAGCCTTAATGGCAC
CAGAGATTATCTGCCAAAAGCCAGGTACCTTTTCCGTCCTTAATTACTCAAAAGCTGACTTATGGGCTGTTGGTGCCATTGCCTATGAGATCTTCAATTG
TCACAATCCATTTTACGGACCTTCTAGATTGAAGAACTTCAACTACAAAGAGGGAGATCTGCCCAAGCTACCTGATGAGGTTCCTACAGTTATCCAGGCA
TTAGTAGCAAACTTACTAAAGCGTAATCCAAACAAACGTTTGGATCCTGAAGTTGCCGCTAATGTTTGTCAACTGTTTCTGTGGGCACCTTCTACTTGGC
TGAAACCTGGTTTGAAGGTTCCGACTTCTGGTGAAATCCTACAATGGCTGCTTTCTCTAACTACTAAGGTTTTGTGCGAAGGCAAGATCAACAACAAATC
TTTCGGTGCTGCCTTTACGCGAAATTGGAGAAGAACTTATCCCGAGTACTTATTGATTTCAAGCTTTCTATGTAGGGCTAAATTGGCTAACGTTAGAAAC
GCTTTGCATTGGATACAGGAAAATCTTCCAGAGCTTGATCATCACCATCATCACCACTAAGCGGCCGC
TCAAAGACGATGAAACCGATGATAACAAGTATCCCTTTGCTCTTAAGATGATGTTCAACTACGACATTCAGTCCAATAGCATGGAGATCTTGAAGGCCAT
GTACAGAGAAACTGTGCCAGCTAGAATGTATTACAGTAATCACGATTTGAACAATTGGGAGATAGAGTTGGCTAACAGAAGGAAACACCTTCCACCGCAT
CCAAACATAGTAGCCATTTTCTCCGTGTTTACCGACTTGTATCCAGCTGCTTTACCACCTAGGTTGCATCCTGAAGGAGAAGGACGAAATATGTCCTTGT
TCCTATTGATGAAGCGTTATGACTGCAATCTTCAAAGCTTCCTTTCTACGGCACCTTCAACAAGAACAAGTCTGTTGTTATTGGCACAACTGCTGGAAGG
AGTCGCACATATGACTGCTCACGGTATTGCTCATAGAGACTTGAAGTCGGACAATTTGTTGCTTGATACATCTGAACCTGAAAGTCCAATTCTGGTGATT
TCTGCTTTTGGCTGTTGTTTGGCAGATAAAACCAATGGGTTATCCTTGCCCTATACCTCGTACGAAATGGATAAAGGTGGGAACACAGCCTTAATGGCAC
CAGAGATTATCTGCCAAAAGCCAGGTACCTTTTCCGTCCTTAATTACTCAAAAGCTGACTTATGGGCTGTTGGTGCCATTGCCTATGAGATCTTCAATTG
TCACAATCCATTTTACGGACCTTCTAGATTGAAGAACTTCAACTACAAAGAGGGAGATCTGCCCAAGCTACCTGATGAGGTTCCTACAGTTATCCAGGCA
TTAGTAGCAAACTTACTAAAGCGTAATCCAAACAAACGTTTGGATCCTGAAGTTGCCGCTAATGTTTGTCAACTGTTTCTGTGGGCACCTTCTACTTGGC
TGAAACCTGGTTTGAAGGTTCCGACTTCTGGTGAAATCCTACAATGGCTGCTTTCTCTAACTACTAAGGTTTTGTGCGAAGGCAAGATCAACAACAAATC
TTTCGGTGCTGCCTTTACGCGAAATTGGAGAAGAACTTATCCCGAGTACTTATTGATTTCAAGCTTTCTATGTAGGGCTAAATTGGCTAACGTTAGAAAC
GCTTTGCATTGGATACAGGAAAATCTTCCAGAGCTTGATCATCACCATCATCACCACTAAGCGGCCGC
Amino Acid Sequence
View Sequence
MRFPSIFTAVLFAASSALAAPVNTTTEDETAQIPAEAVIGYSDLEGDFDVAVLPFSNSTNNGLLFINTTIASIAAKEEGVSLEKREAEAYVEFSRFESNP
ITLNDLSLGKPIAKGTNGVVYSAKVKDDETDDNKYPFALKMMFNYDIQSNSMEILKAMYRETVPARMYYSNHDLNNWEIELANRRKHLPPHPNIVAIFSV
FTDLYPAALPPRLHPEGEGRNMSLFLLMKRYDCNLQSFLSTAPSTRTSLLLLAQLLEGVAHMTAHGIAHRDLKSDNLLLDTSEPESPILVISAFGCCLAD
KTNGLSLPYTSYEMDKGGNTALMAPEIICQKPGTFSVLNYSKADLWAVGAIAYEIFNCHNPFYGPSRLKNFNYKEGDLPKLPDEVPTVIQALVANLLKRN
PNKRLDPEVAANVCQLFLWAPSTWLKPGLKVPTSGEILQWLLSLTTKVLCEGKINNKSFGAAFTRNWRRTYPEYLLISSFLCRAKLANVRNALHWIQENL
PELDHHHHHH*
ITLNDLSLGKPIAKGTNGVVYSAKVKDDETDDNKYPFALKMMFNYDIQSNSMEILKAMYRETVPARMYYSNHDLNNWEIELANRRKHLPPHPNIVAIFSV
FTDLYPAALPPRLHPEGEGRNMSLFLLMKRYDCNLQSFLSTAPSTRTSLLLLAQLLEGVAHMTAHGIAHRDLKSDNLLLDTSEPESPILVISAFGCCLAD
KTNGLSLPYTSYEMDKGGNTALMAPEIICQKPGTFSVLNYSKADLWAVGAIAYEIFNCHNPFYGPSRLKNFNYKEGDLPKLPDEVPTVIQALVANLLKRN
PNKRLDPEVAANVCQLFLWAPSTWLKPGLKVPTSGEILQWLLSLTTKVLCEGKINNKSFGAAFTRNWRRTYPEYLLISSFLCRAKLANVRNALHWIQENL
PELDHHHHHH*
Vector Type
Yeast
Antibiotic
Amp
100042 R 1000 1001 1047 19007
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