PINK1 (Alvinella pompejana)

Plasmid

pET15 6HIS SUMO Pink1 (alvinella)

Parent Plasmid

pET15 6HIS SUMO

Expressed

Alvinella pompejana PINK1 (not full-length)

DU Number

DU51291

Genbank

TERA00314

Tag

SUMO

6His

Species

Alvinella pompejana

Sequence of Insert

View Sequence

GGATTTGTTGTTAGCAATGAAGCACCGGGTGTTACCGCAAGCGTTGAAGCAGGTAGCCAGAGCGATATTGAAGTTATTGATGAACAAGAACTGACCGCAGCAGAAGATTATACCCTGAGCAATCATGCAATTGGTGTTGAAGTTGCAGATACCGGTAGTGATATCGAAGTGATCGAAGATGAATTTCCGCCTGGTATGTTTGTGATCAATAATGAAACCCCTGGTGTGACCGCATCAGTTGAAGCCGGTAATCAGTCAGGTATTGAAATCACCGATGAGCAAGAAGTGATTGTTGCAACCCTGACAGCAGGTCGTTTTCTGCCGAAACAGAAAGTTCATCACGATGTTACCGAAGGTGAAGCCAGCCAGCTGAGCCAGGGTCAGAATCCGGAAGAAAATAGCGATTGGAATATCGCCATCAAAATCATGTATAACTATGGCATTGAAAGCAACGCCAGCGAACTGTATCGTCATATGGCAAAAGAACTGCTGCCTGCACATGCAAATTTTCTGCAAGAAACCCATGAACTGTGGCACAATCGCAAAAAAGAAAATTGTCAGCTGCCTGGCCATCCGAATATTGTTACCATGTATGGTGGTTTCGTTGATGATTTTCCGGTTCTGAGCGAAGCACTGGAAGTTTATCCGTATGCACTGCCTCCGCGTATTAACCCGGAAGGTTGTGGTCGTAATCGTACCATGTTTATTGTGATGAAACGCTATCCGTATACCCTGCGTGAATATCTGCAGGTTAAACATGATCTGAGCTTTCGTCATAGCACCTTACTGCTGCTGCAGCTGGTTGAAGCGGTTGTTCATATGACCGAACATGGTGTTGTTCATCGTGATCTGAAAAGCGATAACATCTTTGTGGAAGAGATCGATGATTGTCCGCATCTGGTTGTTAGCGATTTTGGTGAAAGCATCCATTTCGATCAAGAGGATAATCTGTGTCTGCCGTATATGACGGATGAAATTAACAAAGGTGGTAATAGCGCACTGCAGGCACCGGAAATTGCCTGTGCAGTTCCGGGTCGGAAAAAATTCCTGGATTATCGTAAAAGCGATCTGTGGACCGTTGGCACCCTGGCATATGAAATTTTTGGTATGCCGAACCCGTTTTATGAAACCGTTGATGGTAAACGTCTGCAGAGCGCAACCTATAGCGAAGATGATCTGCCGAGCCTGGATCATGTTCCGGATGTTCTGGCATGGCTGATTCGTAATATGCTGAGCCGTGATCCTGATCAGCGTTGTAGTCCGCGTCAGATTAGCGTTTGTCTGCATCTGATTCTGTGGTCACCGCTGGAATGGACCGTTAGCCTGAATGTTCGTAGCGCATATAAACTGACCTATCCGCCTACCGAACTGAATCTGTATCGTTGGATTATGCTGCTGACCGCACAGGTTCTGCTGGAAAAACTGATTTGTTATCAGCGTGTTGAACATGAACTGCGTCGTCTGTTTCTGAGCCACTTTGATTTCAAAGAACTGGTTCAGTGCGTGAAACTGTTTGAAAACGTGAACTAAGCGGCCGC

Amino Acid Sequence

View Sequence

MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTHLKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGFVVSNEAPGVTASVEAGSQSDIEVIDEQELTAAEDYTLSNHAIGVEVADTGSDIEVIEDEFPPGMFVINNETPGVTASVEAGNQSGIEITDEQEVI
VATLTAGRFLPKQKVHHDVTEGEASQLSQGQNPEENSDWNIAIKIMYNYGIESNASELYRHMAKELLPAHANFLQETHELWHNRKKENCQLPGHPNIVTM
YGGFVDDFPVLSEALEVYPYALPPRINPEGCGRNRTMFIVMKRYPYTLREYLQVKHDLSFRHSTLLLLQLVEAVVHMTEHGVVHRDLKSDNIFVEEIDDC
PHLVVSDFGESIHFDQEDNLCLPYMTDEINKGGNSALQAPEIACAVPGRKKFLDYRKSDLWTVGTLAYEIFGMPNPFYETVDGKRLQSATYSEDDLPSLD
HVPDVLAWLIRNMLSRDPDQRCSPRQISVCLHLILWSPLEWTVSLNVRSAYKLTYPPTELNLYRWIMLLTAQVLLEKLICYQRVEHELRRLFLSHFDFKE
LVQCVKLFENVN

Vector Type

Bacterial

Antibiotic

Amp

Comments

codon optimised for E.coli expression. urgent timescale. Sequence and links to be emailed.

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