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PINK1 (human)

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Plasmid
pET6HIS SUMO Pink1 214-581 (opt) D384A
Parent Plasmid
pET15b 6His SUMO
Expressed
6HIS SUMO Pink1 214-581 (opt) D384A
DU Number
DU51170
Tag
6His
SUMO
Species
Human
Sequence of Insert
ATGGGTCATCATCACCATCACCATTCTGACCAGGAGGCAAAACCTTCAACTGAGGACTTGGGGGATAAGAAGGAAGGTGAATATATTAAACTCAAAGTCA
TTGGACAGGATAGCAGTGAGATTCACTTCAAAGTGAAAATGACAACACATCTCAAGAAACTCAAAGAATCATACTGTCAAAGACAGGGTGTTCCAATGAA
CTCACTCAGGTTTCTCTTTGAGGGTCAGAGAATTGCTGATAATCATACTCCAAAAGAACTGGGAATGGAGGAAGAAGATGTGATTGAAGTTTATCAGGAA
CAAACGGGGGGATTTCCGCTGGCCATTAAAATGATGTGGAATATTAGCGCAGGTAGCAGCAGCGAAGCAATTCTGAATACCATGAGCCAAGAACTGGTTC
CGGCAAGCCGTGTTGCACTGGCAGGCGAATATGGTGCAGTTACCTATCGTAAAAGTAAACGTGGTCCGAAACAGCTGGCACCGCATCCGAACATTATTCG
TGTTCTGCGTGCATTTACCAGCAGCGTTCCGCTGCTGCCAGGTGCACTGGTTGATTATCCGGATGTTCTGCCGAGCCGTCTGCATCCGGAAGGCCTGGGC
CATGGTCGTACCCTGTTTCTGGTTATGAAAAACTATCCGTGTACCCTGCGTCAGTATCTGTGTGTTAATACCCCGAGTCCTCGCCTGGCAGCCATGATGC
TGCTGCAGCTGCTGGAAGGTGTTGATCATCTGGTTCAGCAGGGTATTGCACATCGTGATCTGAAAAGCGATAATATTCTGGTTGAACTGGACCCGGATGG
TTGTCCGTGGCTGGTTATTGCAGCTTTTGGTTGTTGTCTGGCAGATGAAAGCATTGGTCTGCAACTGCCGTTTAGCAGCTGGTATGTTGATCGTGGTGGT
AATGGTTGTCTGATGGCACCGGAAGTTAGCACAGCACGTCCTGGTCCGCGTGCAGTTATTGATTATAGCAAAGCAGATGCATGGGCAGTTGGTGCAATTG
CCTATGAAATTTTTGGTCTGGTGAATCCGTTTTATGGTCAGGGTAAAGCACATCTGGAAAGCCGTAGCTATCAAGAAGCACAGCTGCCTGCACTGCCGGA
AAGCGTTCCTCCGGATGTGCGTCAGCTGGTTCGTGCCCTGCTGCAGCGCGAAGCCAGCAAACGTCCGAGCGCACGTGTTGCAGCAAATGTTCTGCATCTG
AGCCTGTGGGGTGAACATATTCTGGCACTGAAAAATCTGAAACTGGATAAAATGGTTGGTTGGCTGCTGCAACAGAGCGCAGCAACCCTGCTGGCAAATC
GTCTGACCGAAAAATGTTGTGTTGAAACCAAAATGAAAATGCTGTTCCTGGCCAATCTGGAATGTGAAACCCTGTGTCAGGCAGCCCTGCTGCTGTGTAG
CTGGCGTGCAGCACTGTAAGCGGCCGC
Amino Acid Sequence
MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTHLKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGFPLAIKMMWNISAGSSSEAILNTMSQELVPASRVALAGEYGAVTYRKSKRGPKQLAPHPNIIRVLRAFTSSVPLLPGALVDYPDVLPSRLHPEGLG
HGRTLFLVMKNYPCTLRQYLCVNTPSPRLAAMMLLQLLEGVDHLVQQGIAHRDLKSDNILVELDPDGCPWLVIAAFGCCLADESIGLQLPFSSWYVDRGG
NGCLMAPEVSTARPGPRAVIDYSKADAWAVGAIAYEIFGLVNPFYGQGKAHLESRSYQEAQLPALPESVPPDVRQLVRALLQREASKRPSARVAANVLHL
SLWGEHILALKNLKLDKMVGWLLQQSAATLLANRLTEKCCVETKMKMLFLANLECETLCQAALLLCSWRAAL*
Vector Type
Bacterial
Antibiotic
Amp
Comments
based on DU3545 and 43230. It has to be optimised for bacterial expression.
100042 R 1000 1001 1047 19007
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