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PINK1 (Tribolium) Codon optimised sequence

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Plasmid
pET6HIS SUMO Pink1 tribolium 150-570 S205D D359A E527A K528A
Parent Plasmid
pET15b 6His SUMO1
Expressed
6HIS SUMO Pink1 tribolium 150-570 S205D D359A E527A K528A
DU Number
DU51142
Tag
6His
SUMO
Species
Tribolium castaneum
Sequence of Insert
AGCCGCTTTGAATCTAACCCGATCACCCTGAATGATCTGAGTCTGGGTAAACCGATCGCCAAAGGCACGAACGGTGTGGTTTACTCCGCAAAAGTCAAAG
ATGACGAAACCGATGACAATAAATATCCGTTTGCCCTGAAAATGATGTTCAACTACGATATTCAGGACAATTCTATGGAAATCCTGAAAGCTATGTATCG
TGAAACCGTTCCGGCGCGCATGTATTACTCAAACCACGACCTGAACAATTGGGAAATTGAACTGGCTAACCGTCGCAAACATCTGCCGCCGCACCCGAAT
ATTGTGGCAATCTTTAGCGTTTTCACCGATCTGATCCAAGAACTGGAAGGTTCTAAAGACCTGTATCCGGCAGCGCTGCCGCCGCGTCTGCATCCGGAAG
GTGAAGGTCGCAACATGAGCCTGTTTCTGCTGATGAAACGTTATGATTGTAATCTGCAGTCATTCCTGAGCACCGCTCCGAGTACCCGCACCTCCCTGCT
GCTGCTGGCCCAACTGCTGGAAGGCGTGGCGCATATGACGGCCCACGGTATTGCACATCGTGATCTGAAATCAGACAACCTGCTGCTGGATACCTCAGAA
CCGGAATCGCCGATTCTGGTTATCTCGGCTTTTGGCTGCTGTCTGGCGGACAAAACCAACGGTCTGAGCCTGCCGTATACGTCTTACGAAATGGATAAAG
GCGGTAATACGGCGCTGATGGCCCCGGAAATTATCTGCCAGAAACCGGGCACCTTTAGTGTCCTGAATTATTCCAAAGCTGACCTGTGGGCGGTGGGTGC
AATCGCTTACGAAATCTTCAACTGTCACAATCCGTTCTACGGCCCGTCTCGCCTGAAAAACTTCAACTACAAAGAAGGTGATCTGCCGAAACTGCCGGAC
GAAGTGCCGACCGTTATTCAGGCACTGGTGGCTAACCTGCTGAAACGTAACCCGAATAAACGCCTGGACCCGGAAGTTGCGGCCAATGTCTGCCAGCTGT
TTCTGTGGGCACCGAGTACCTGGCTGAAACCGGGTCTGAAAGTTCCGACGTCAGGTGAAATTCTGCAATGGCTGCTGTCGCTGACCACGAAAGTCCTGTG
CGAAGGCAAAATCAACAACAAATCCTTCGGTGCAGCATTCACCCGTAATTGGCGTCGCACGTATCCGGAATACCTGCTGATTAGTTCCTTCCTGTGTCGC
GCGAAACTGGCTAATGTTCGTAATGCTCTGCACTGGATTCAAGAAAACCTGCCGGAACTGGATTAAGCGGCCGCGTAAAGATCCGGCTGCTAACAAAGCC
CGAAAGAGGCTGAATGG
Amino Acid Sequence
MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTHLKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGSRFESNPITLNDLSLGKPIAKGTNGVVYSAKVKDDETDDNKYPFALKMMFNYDIQDNSMEILKAMYRETVPARMYYSNHDLNNWEIELANRRKHLP
PHPNIVAIFSVFTDLIQELEGSKDLYPAALPPRLHPEGEGRNMSLFLLMKRYDCNLQSFLSTAPSTRTSLLLLAQLLEGVAHMTAHGIAHRDLKSDNLLL
DTSEPESPILVISAFGCCLADKTNGLSLPYTSYEMDKGGNTALMAPEIICQKPGTFSVLNYSKADLWAVGAIAYEIFNCHNPFYGPSRLKNFNYKEGDLP
KLPDEVPTVIQALVANLLKRNPNKRLDPEVAANVCQLFLWAPSTWLKPGLKVPTSGEILQWLLSLTTKVLCEGKINNKSFGAAFTRNWRRTYPEYLLISS
FLCRAKLANVRNALHWIQENLPELD*
Vector Type
Bacterial
Antibiotic
Amp
Comments
based on DU 46263
100042 R 1000 1001 1047 19007
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