PINK1

CRISPR guide plasmid. Targets sense strand of last coding exon, designed to KI and in-frame YFP reporter when co-transfected with antisense guide 48947 and donor 48992. Guide sequence shown as sequence of insert. Positives may be enriched for with puromycin prior to further selection by western blotting and FACs. Note this system does not require induction with Tet/Dox. May be used in any cell-line though an empty TRex line is preferable as this allows for downstream rescue and mutant analysis if necessary.