LRRK2 (mouse)

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Synonyms
4921513O20Rik, 9330188B09Rik, AW561911, D630001M17Rik, Gm927, cI-46
Plasmid
pKN7 LRRK2m KO sense B
Parent Plasmid
pKN7
Expressed
LRRK2m KO sense guide B
DU Number
DU48892
Genbank
NM_025730.3
Species
Mouse
Sequence of Insert
GCTCTGAAGAAGTTGATAGTC



Vector Type
Mammalian
Antibiotic
Kan
Comments
CRISPR guide plasmid. Targets sense strand in exon 1, designed to KO LRRK2m when co-transfected with antisense guide 48900. Guide sequence shown as sequence of insert. Positives may be enriched for with puromycin prior to further selection by western blotting. Note this system does not require induction with Tet/Dox. May be used in any cell-line though an empty flp-in line is preferable as this allows for downstream rescue and mutant analysis if necessary.
100042 R 1000 1001 1047 19007
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