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PINK1 (Pediculus)

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Plasmid
pET15b 6HIS SUMO Pediculus Pink S115-end
Parent Plasmid
pET15b 6His SUMO
Expressed
6HIS SUMO Pediculus Pink S115-end
DU Number
DU26140
Tag
6His
SUMO
Species
Pediculus humanus
Sequence of Insert
GGATCCTCCGGTCTGCTGACCAAAGATGACGAACTGGAAGGCATTTGCTGGGAAATCCGCGAAGCAGTTAGCAAGGGTAAATGGAACGATTCAGAATCGGAAAATGTCGAACAGCTGCAAGCAGCTAATCTGGATGAACTGGACCTGGGCGAACCGATTGCAAAAGGTTGTAACGCTGTGGTTTATTCTGCGAAACTGAAAAACGTGCAGAGTAATAAACTGGCGCACCAACTGGCCGTGAAAATGATGTTCAACTACGATGTTGAAAGCAATTCTACCGCAATTCTGAAAGCTATGTATCGTGAAACGGTTCCGGCGATGTCTTACTTTTTCAACCAGAACCTGTTTAACATCGAAAACATCAGTGATTTCAAAATTCGCCTGCCGCCGCATCCGAATATCGTTCGTATGTATAGCGTCTTTGCCGATCGCATTCCGGACCTGCAGTGCAACAAACAACTGTACCCGGAAGCACTGCCGCCGCGTATCAACCCGGAAGGCTCTGGTCGCAATATGAGTCTGTTCCTGGTCATGAAACGTTATGATTGTACCCTGAAAGAATACCTGCGTGACAAAACGCCGAACATGCGCAGCTCTATTCTGCTGCTGTCCCAGCTGCTGGAAGCAGTTGCTCATATGAACATTCACAATATCTCCCATCGCGATCTGAAATCAGACAATATCCTGGTCGATCTGAGCGAAGGCGACGCCTATCCGACCATTGTGATCACGGATTTTGGCTGCTGTCTGTGCGACAAACAGAACGGTCTGGTGATTCCGTACCGTAGCGAAGATCAAGACAAAGGCGGTAACCGTGCCCTGATGGCACCGGAAATCGCGAATGCCAAACCGGGCACCTTCTCGTGGCTGAACTATAAAAAATCTGATCTGTGGGCGGTTGGTGCAATCGCTTACGAAATCTTCAACATCGATAACCCGTTCTACGACAAAACCATGAAACTGCTGAGTAAATCCTATAAAGAAGAAGATCTGCCGGAACTGCCGGACACGATCCCGTTTATTATCCGCAACCTGGTGTCTAATATGCTGTCACGTTCGACCAATAAACGCCTGGATTGTGATGTTGCCGCAACCGTGGCACAGCTGTATCTGTGGGCACCGAGTTCCTGGCTGAAAGAAAACTACACCCTGCCGAACAGTAATGAAATTATCCAATGGCTGCTGTGCCTGTCATCGAAAGTGCTGTGTGAACGTGATATTACGGCTCGCAACAAAACCAATACGATGAGCGAAAGCGTGAGTAAAGCGCAGTATAAAGGTCGTCGCTCCCTGCCGGAATACGAACTGATCGCGTCATTCCTGCGTCGCGTCCGTCTGCATCTGGTCCGTAAAGGTCTGAAATGGATTCAAGAACTGCACATCTACAACTAAGCGGCCGC
Amino Acid Sequence
MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTHLKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQEQTGGSGLLTKDDELEGICWEIREAVSKGKWNDSESENVEQLQAANLDELDLGEPIAKGCNAVVYSAKLKNVQSNKLAHQLAVKMMFNYDVESNSTAILKAMYRETVPAMSYFFNQNLFNIENISDFKIRLPPHPNIVRMYSVFADRIPDLQCNKQLYPEALPPRINPEGSGRNMSLFLVMKRYDCTLKEYLRDKTPNMRSSILLLSQLLEAVAHMNIHNISHRDLKSDNILVDLSEGDAYPTIVITDFGCCLCDKQNGLVIPYRSEDQDKGGNRALMAPEIANAKPGTFSWLNYKKSDLWAVGAIAYEIFNIDNPFYDKTMKLLSKSYKEEDLPELPDTIPFIIRNLVSNMLSRSTNKRLDCDVAATVAQLYLWAPSSWLKENYTLPNSNEIIQWLLCLSSKVLCERDITARNKTNTMSESVSKAQYKGRRSLPEYELIASFLRRVRLHLVRKGLKWIQELHIYN*
Vector Type
Bacterial
Antibiotic
Amp
Comments
Linker after SENP cleavage deleted.
Codon optimised sequence
100042 R 1000 1001 1047 19007
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