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Synonyms
AR-JP; LPRS2; PDJ; PRKN; PARK2 , E2-F1; L-UBC; UBCH7; UbcM4
Plasmid
pET15b 6His sumo UBE2L3-Parkin S127-V465(end)
Parent Plasmid
pET15b 6His SUMO
Expressed
6His sumo UBE2L3-Parkin S127-V465(end)
DU Number
DU50097
Genbank
NM_004562.2 GI:169790968 (Parkin), NM_003347.3 GI:373432685 with silent change a423g (UBE2L3)
Tag
6His
SUMO
Species
Human
Sequence of Insert
View Sequence
GGATCCATGGCGGCCAGCAGGAGGCTGATGAAGGAGCTTGAAGAAATCCGCAAATGTGGGATGAAAAACTTCCGTAACATCCAGGTTGATGAAGCTAATTTATTGACTTGGCAAGGGCTTATTGTTCCTGACAACCCTCCATATGATAAGGGAGCCTTCAGAATCGAAATCAACTTTCCAGCAGAGTACCCATTCAAACCACCGAAGATCACATTTAAAACAAAGATCTATCACCCAAACATCGACGAAAAGGGGCAGGTCTGTCTGCCAGTAATTAGTGCCGAAAACTGGAAGCCAGCAACCAAAACCGACCAAGTAATCCAGTCCCTCATAGCACTGGTGAATGACCCCCAGCCTGAGCACCCGCTTCGGGCTGACCTAGCTGAAGAATACTCTAAGGACCGTAAAAAATTCTGTAAGAATGCTGAGGAGTTTACAAAGAAATATGGGGAAAAGCGACCTGTGGACGGATCC
Amino Acid Sequence
View Sequence
MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTH
LKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGSMAASRRLMKELEEIRKCGMKNFRNIQVDEANLLTWQGLIVPDNPP
YDKGAFRIEINFPAEYPFKPPKITFKTKIYHPNIDEKGQVCLPVISAENW
KPATKTDQVIQSLIALVNDPQPEHPLRADLAEEYSKDRKKFCKNAEEFTK
KYGEKRPVDGSSRKDSPPAGSPAGRSIYNSFYVYCKGPCQRVQPGKLRVQ
CSTCRQATLTLTQGPSCWDDVLIPNRMSGECQSPHCPGTSAEFFFKCGAH
PTSDKETSVALHLIATNSRNITCITCTDVRSPVLVFQCNSRHVICLDCFH
LYCVTRLNDRQFVHDPQLGYSLPCVAGCPNSLIKELHHFRILGEEQYNRY
QQYGAEECVLQMGGVLCPRPGCGAGLLPEPDQRKVTCEGGNGLGCGFAFC
RECKEAYHEGECSAVFEASGTTTQAYRVDERAAEQARWEAASKETIKKTT
KPCPRCHVPVEKNGGCMHMKCPQPQCRLEWCWNCGCEWNRVCMGDHWFDV
*
LKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGSMAASRRLMKELEEIRKCGMKNFRNIQVDEANLLTWQGLIVPDNPP
YDKGAFRIEINFPAEYPFKPPKITFKTKIYHPNIDEKGQVCLPVISAENW
KPATKTDQVIQSLIALVNDPQPEHPLRADLAEEYSKDRKKFCKNAEEFTK
KYGEKRPVDGSSRKDSPPAGSPAGRSIYNSFYVYCKGPCQRVQPGKLRVQ
CSTCRQATLTLTQGPSCWDDVLIPNRMSGECQSPHCPGTSAEFFFKCGAH
PTSDKETSVALHLIATNSRNITCITCTDVRSPVLVFQCNSRHVICLDCFH
LYCVTRLNDRQFVHDPQLGYSLPCVAGCPNSLIKELHHFRILGEEQYNRY
QQYGAEECVLQMGGVLCPRPGCGAGLLPEPDQRKVTCEGGNGLGCGFAFC
RECKEAYHEGECSAVFEASGTTTQAYRVDERAAEQARWEAASKETIKKTT
KPCPRCHVPVEKNGGCMHMKCPQPQCRLEWCWNCGCEWNRVCMGDHWFDV
*
Vector Type
Bacterial
Antibiotic
Amp
Comments
UBE2L3 cloned into BamHI site of DU50079. Sumo not flush, as agreed with requester.
100042 R 1000 1001 1047 19007
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