PINK1 | LRRK2 Resource

Plasmid

pcDNA5 FRT/TO FLAG Pink1 110-581 Fis1 101-end

Parent Plasmid

pcDNA5 FRT/TO

Expressed

FLAG-PINK1 (110-581)-FIS1 (101-end)

DU Number

DU46897

Genbank

Q9BXM7

Tag

FLAG

Species

Human

Sequence of Insert

View Sequence

GGATCCGCCACCATGCTCATCGAGGAAAAACAGGCGGAGAGCCGGCGGGC
GGTCTCGGCCTGTCAGGAGATCCAGGCAATTTTTACCCAGAAAAGCAAGC
CGGGGCCTGACCCGTTGGACACGAGACGCTTGCAGGGCTTTCGGCTGGAG
GAGTATCTGATAGGGCAGTCCATTGGTAAGGGCTGCAGTGCTGCTGTGTA
TGAAGCCACCATGCCTACATTGCCCCAGAACCTGGAGGTGACAAAGAGCA
CCGGGTTGCTTCCAGGGAGAGGCCCAGGTACCAGTGCACCAGGAGAAGGG
CAGGAGCGAGCTCCGGGGGCCCCTGCCTTCCCCTTGGCCATCAAGATGAT
GTGGAACATCTCGGCAGGTTCCTCCAGCGAAGCCATCTTGAACACAATGA
GCCAGGAGCTGGTCCCAGCGAGCCGAGTGGCCTTGGCTGGGGAGTATGGA
GCAGTCACTTACAGAAAATCCAAGAGAGGTCCCAAGCAACTAGCCCCTCA
CCCCAACATCATCCGGGTTCTCCGCGCCTTCACCTCTTCCGTGCCGCTGC
TGCCAGGGGCCCTGGTCGACTACCCTGATGTGCTGCCCTCACGCCTCCAC
CCTGAAGGCCTGGGCCATGGCCGGACGCTGTTCCTCGTTATGAAGAACTA
TCCCTGTACCCTGCGCCAGTACCTTTGTGTGAACACACCCAGCCCCCGCC
TCGCCGCCATGATGCTGCTGCAGCTGCTGGAAGGCGTGGACCATCTGGTT
CAACAGGGCATCGCGCACAGAGACCTGAAATCCGACAACATCCTTGTGGA
GCTGGACCCAGACGGCTGCCCCTGGCTGGTGATCGCAGATTTTGGCTGCT
GCCTGGCTGATGAGAGCATCGGCCTGCAGTTGCCCTTCAGCAGCTGGTAC
GTGGATCGGGGCGGAAACGGCTGTCTGATGGCCCCAGAGGTGTCCACGGC
CCGTCCTGGCCCCAGGGCAGTGATTGACTACAGCAAGGCTGATGCCTGGG
CAGTGGGAGCCATCGCCTATGAAATCTTCGGGCTTGTCAATCCCTTCTAC
GGCCAGGGCAAGGCCCACCTTGAAAGCCGCAGCTACCAAGAGGCTCAGCT
ACCTGCACTGCCCGAGTCAGTGCCTCCAGACGTGAGACAGTTGGTGAGGG
CACTGCTCCAGCGAGAGGCCAGCAAGAGACCATCTGCCCGAGTAGCCGCA
AATGTGCTTCATCTAAGCCTCTGGGGTGAACATATTCTAGCCCTGAAGAA
TCTGAAGTTAGACAAGATGGTTGGCTGGCTCCTCCAACAATCGGCCGCCA
CTTTGTTGGCCAACAGGCTCACAGAGAAGTGTTGTGTGGAAACAAAAATG
AAGATGCTCTTTCTGGCTAACCTGGAGTGTGAAACGCTCTGCCAGGCAGC
CCTCCTCCTCTGCTCATGGAGGGCAGCCCTGGCTAGCGAGCCCCAGAACA
ACCAGGCCAAGGAACTGGAGCGGCTCATTGACAAGGCCATGAAGAAAGAT
GGACTCGTGGGCATGGCCATCGTGGGAGGCATGGCCCTGGGTGTGGCGGG
ACTGGCCGGACTCATCGGACTTGCTGTGTCCAAGTCCAAATCCTGAGCGG
CCGC

Amino Acid Sequence

View Sequence

MLIEEKQAESRRAVSACQEIQAIFTQKSKPGPDPLDTRRLQGFRLEEYLI
GQSIGKGCSAAVYEATMPTLPQNLEVTKSTGLLPGRGPGTSAPGEGQERA
PGAPAFPLAIKMMWNISAGSSSEAILNTMSQELVPASRVALAGEYGAVTY
RKSKRGPKQLAPHPNIIRVLRAFTSSVPLLPGALVDYPDVLPSRLHPEGL
GHGRTLFLVMKNYPCTLRQYLCVNTPSPRLAAMMLLQLLEGVDHLVQQGI
AHRDLKSDNILVELDPDGCPWLVIADFGCCLADESIGLQLPFSSWYVDRG
GNGCLMAPEVSTARPGPRAVIDYSKADAWAVGAIAYEIFGLVNPFYGQGK
AHLESRSYQEAQLPALPESVPPDVRQLVRALLQREASKRPSARVAANVLH
LSLWGEHILALKNLKLDKMVGWLLQQSAATLLANRLTEKCCVETKMKMLF
LANLECETLCQAALLLCSWRAALASEPQNNQAKELERLIDKAMKKDGLVG
MAIVGGMALGVAGLAGLIGLAVSKSKS*

Vector Type

Mammalian

Antibiotic

Amp

Comments

in pcDNA5FRT/TO - modified DU43548

100042 R 1000 1001 1047 19007