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Plasmid
pMAL4C Pink1 123-581 T185E S187E T257E S284E E417G
Parent Plasmid
pMAL4C
Expressed
MBP Pink1 123-581 T185E S187E T257E S284E E417G
DU Number
DU46419
Genbank
Q9BXM7
Tag
MBP
Species
Human
Sequence of Insert
View Sequence
CTCGGCCTGTCAGGAGATCCAGGCAATTTTTACCCAGAAAAGCAAGCCGG
GGCCTGACCCGTTGGACACGAGACGCTTGCAGGGCTTTAGGCTGGAGGAG
TATCTGATAGGGCAGTCCATTGGTAAGGGCTGCAGTGCTGCTGTGTATGA
AGCCACCATGCCTACATTGCCCCAGAACCTGGAGGTGGAAAAGGAAACCG
GGTTGCTTCCAGGGAGAGGCCCAGGTACCAGTGCACCAGGAGAAGGGCAG
GAGCGAGCTCCGGGGGCCCCTGCCTTCCCCTTGGCCATCAAGATGATGTG
GAACATCTCGGCAGGTTCCTCCAGCGAAGCCATCTTGAACACAATGAGCC
AGGAGCTGGTCCCAGCGAGCCGAGTGGCCTTGGCTGGGGAGTATGGAGCA
GTCGAATACAGAAAATCCAAGAGAGGTCCCAAGCAACTAGCCCCTCACCC
CAACATCATCCGGGTTCTCCGCGCCTTCACCTCTGAAGTGCCGCTGCTGC
CAGGGGCCCTGGTCGACTACCCTGATGTGCTGCCCTCACGCCTCCACCCT
GAAGGCCTGGGCCATGGCCGGACGCTGTTCCTCGTTATGAAGAACTATCC
CTGTACCCTGCGCCAGTACCTTTGTGTGAACACACCCAGCCCCCGCCTCG
CCGCCATGATGCTGCTGCAGCTGCTGGAAGGCGTGGACCATCTGGTTCAA
CAGGGCATCGCGCACAGAGACCTGAAATCCGACAACATCCTTGTGGAGCT
GGACCCAGACGGCTGCCCCTGGCTGGTGAtcgcagattttggctgctgcc
tggctgatgagagcatcggcctgcagttgcccttcagcagctggtacgtg
gatcggggcggaaacggctgtctgatggccccaggggtgtccacggcccg
tcctggccccagggcagtgattgactacagcaaggctgatgcctgggcag
tgggagccatcgcctatgaaatcttcgggcttgtcaatcccttctacggc
cagggcaaggcccaccttgaaagccgcagctaccaagaggctcagctacc
tgcactgcccgagtcagtgcctccagacgtgagacagttggtgagggcac
tgctccagcgagaggccagcaagagaccatctgcccgagtagccgcaaat
gtgcttcatctaagcctctggggtgaacatattctagccctgaagaatct
gaagttagacaagatggttggctggctcctccaacaatcggccgccactt
tgttggccaacaggctcacagagaagtgttgtgtggaaacaaaaatgaag
atgctctttctggctaacctggagtgtgaaacgctctgccaggcagccct
cctcctctgctcatggagggcagccctgtgagcggccgc
GGCCTGACCCGTTGGACACGAGACGCTTGCAGGGCTTTAGGCTGGAGGAG
TATCTGATAGGGCAGTCCATTGGTAAGGGCTGCAGTGCTGCTGTGTATGA
AGCCACCATGCCTACATTGCCCCAGAACCTGGAGGTGGAAAAGGAAACCG
GGTTGCTTCCAGGGAGAGGCCCAGGTACCAGTGCACCAGGAGAAGGGCAG
GAGCGAGCTCCGGGGGCCCCTGCCTTCCCCTTGGCCATCAAGATGATGTG
GAACATCTCGGCAGGTTCCTCCAGCGAAGCCATCTTGAACACAATGAGCC
AGGAGCTGGTCCCAGCGAGCCGAGTGGCCTTGGCTGGGGAGTATGGAGCA
GTCGAATACAGAAAATCCAAGAGAGGTCCCAAGCAACTAGCCCCTCACCC
CAACATCATCCGGGTTCTCCGCGCCTTCACCTCTGAAGTGCCGCTGCTGC
CAGGGGCCCTGGTCGACTACCCTGATGTGCTGCCCTCACGCCTCCACCCT
GAAGGCCTGGGCCATGGCCGGACGCTGTTCCTCGTTATGAAGAACTATCC
CTGTACCCTGCGCCAGTACCTTTGTGTGAACACACCCAGCCCCCGCCTCG
CCGCCATGATGCTGCTGCAGCTGCTGGAAGGCGTGGACCATCTGGTTCAA
CAGGGCATCGCGCACAGAGACCTGAAATCCGACAACATCCTTGTGGAGCT
GGACCCAGACGGCTGCCCCTGGCTGGTGAtcgcagattttggctgctgcc
tggctgatgagagcatcggcctgcagttgcccttcagcagctggtacgtg
gatcggggcggaaacggctgtctgatggccccaggggtgtccacggcccg
tcctggccccagggcagtgattgactacagcaaggctgatgcctgggcag
tgggagccatcgcctatgaaatcttcgggcttgtcaatcccttctacggc
cagggcaaggcccaccttgaaagccgcagctaccaagaggctcagctacc
tgcactgcccgagtcagtgcctccagacgtgagacagttggtgagggcac
tgctccagcgagaggccagcaagagaccatctgcccgagtagccgcaaat
gtgcttcatctaagcctctggggtgaacatattctagccctgaagaatct
gaagttagacaagatggttggctggctcctccaacaatcggccgccactt
tgttggccaacaggctcacagagaagtgttgtgtggaaacaaaaatgaag
atgctctttctggctaacctggagtgtgaaacgctctgccaggcagccct
cctcctctgctcatggagggcagccctgtgagcggccgc
Amino Acid Sequence
View Sequence
MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQ
VAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRY
NGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMF
NLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLI
KNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPT
FKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPL
GAVALKSYEEELVKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVIN
AASGRQTVDEALKDAQTNSSSNNNNNNNNNNLGDDDDKVPEFLEVLFQGP
GSATSACQEIQAIFTQKSKPGPDPLDTRRLQGFRLEEYLIGQSIGKGCSA
AVYEATMPTLPQNLEVEKETGLLPGRGPGTSAPGEGQERAPGAPAFPLAI
KMMWNISAGSSSEAILNTMSQELVPASRVALAGEYGAVEYRKSKRGPKQL
APHPNIIRVLRAFTSEVPLLPGALVDYPDVLPSRLHPEGLGHGRTLFLVM
KNYPCTLRQYLCVNTPSPRLAAMMLLQLLEGVDHLVQQGIAHRDLKSDNI
LVELDPDGCPWLVIADFGCCLADESIGLQLPFSSWYVDRGGNGCLMAPGV
STARPGPRAVIDYSKADAWAVGAIAYEIFGLVNPFYGQGKAHLESRSYQE
AQLPALPESVPPDVRQLVRALLQREASKRPSARVAANVLHLSLWGEHILA
LKNLKLDKMVGWLLQQSAATLLANRLTEKCCVETKMKMLFLANLECETLC
QAALLLCSWRAAL*
Vector Type
Bacterial
Antibiotic
Amp
Comments
Please introduce the following mutations: E417G into the existing clone DU43815 (MBP-human PINK1 T185E/S187E/T257E/S284E (residues 123-end) in pMAL4C vector)
100042 R 1000 1001 1047 19007
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