PINK1 | LRRK2 Resource

Plasmid

pMAL4C Pink1 123-581 T185E S187E T257E S284E L369P

Parent Plasmid

pMAL4C

Expressed

MBP-human PINK1/T185E/S187E/T257E/S284E/L369P (residues 123-end)

DU Number

DU46382

Genbank

Q9BXM7

Tag

MBP

Species

Human

Sequence of Insert

View Sequence

CTCGGCCTGTCAGGAGATCCAGGCAATTTTTACCCAGAAAAGCAAGCCGG
GGCCTGACCCGTTGGACACGAGACGCTTGCAGGGCTTTAGGCTGGAGGAG
TATCTGATAGGGCAGTCCATTGGTAAGGGCTGCAGTGCTGCTGTGTATGA
AGCCACCATGCCTACATTGCCCCAGAACCTGGAGGTGGAAAAGGAAACCG
GGTTGCTTCCAGGGAGAGGCCCAGGTACCAGTGCACCAGGAGAAGGGCAG
GAGCGAGCTCCGGGGGCCCCTGCCTTCCCCTTGGCCATCAAGATGATGTG
GAACATCTCGGCAGGTTCCTCCAGCGAAGCCATCTTGAACACAATGAGCC
AGGAGCTGGTCCCAGCGAGCCGAGTGGCCTTGGCTGGGGAGTATGGAGCA
GTCGAATACAGAAAATCCAAGAGAGGTCCCAAGCAACTAGCCCCTCACCC
CAACATCATCCGGGTTCTCCGCGCCTTCACCTCTGAAGTGCCGCTGCTGC
CAGGGGCCCTGGTCGACTACCCTGATGTGCTGCCCTCACGCCTCCACCCT
GAAGGCCTGGGCCATGGCCGGACGCTGTTCCTCGTTATGAAGAACTATCC
CTGTACCCTGCGCCAGTACCTTTGTGTGAACACACCCAGCCCCCGCCTCG
CCGCCATGATGCTGCTGCAGCTGCTGGAAGGCGTGGACCATCTGGTTCAA
CAGGGCATCGCGCACAGAGACCTGAAATCCGACAACATCCCTGTGGAGCT
GGACCCAGACGGCTGCCCCTGGCTGGTGATCGCAGATTTTGGCTGCTGCC
TGGCTGATGAGAGCATCGGCCTGCAGTTGCCCTTCAGCAGCTGGTACGTG
GATCGGGGCGGAAACGGCTGTCTGATGGCCCCAGAGGTGTCCACGGCCCG
TCCTGGCCCCAGGGCAGTGATTGACTACAGCAAGGCTGATGCCTGGGCAG
TGGGAGCCATCGCCTATGAAATCTTCGGGCTTGTCAATCCCTTCTACGGC
CAGGGCAAGGCCCACCTTGAAAGCCGCAGCTACCAAGAGGCTCAGCTACC
TGCACTGCCCGAGTCAGTGCCTCCAGACGTGAGACAGTTGGTGAGGGCAC
TGCTCCAGCGAGAGGCCAGCAAGAGACCATCTGCCCGAGTAGCCGCAAAT
GTGCTTCATCTAAGCCTCTGGGGTGAACATATTCTAGCCCTGAAGAATCT
GAAGTTAGACAAGATGGTTGGCTGGCTCCTCCAACAATCGGCCGCCACTT
TGTTGGCCAACAGGCTCACAGAGAAGTGTTGTGTGGAAACAAAAATGAAG
ATGCTCTTTCTGGCTAACCTGGAGTGTGAAACGCTCTGCCAGGCAGCCCT
CCTCCTCTGCTCATGGAGGGCAGCCCTGTGATACAAGGACGACGATGACA
AGTAGCGGCCGC

Amino Acid Sequence

View Sequence

MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQ
VAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRY
NGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMF
NLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLI
KNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPT
FKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPL
GAVALKSYEEELVKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVIN
AASGRQTVDEALKDAQTNSSSNNNNNNNNNNLGDDDDKVPEFLEVLFQGP
GSATSACQEIQAIFTQKSKPGPDPLDTRRLQGFRLEEYLIGQSIGKGCSA
AVYEATMPTLPQNLEVEKETGLLPGRGPGTSAPGEGQERAPGAPAFPLAI
KMMWNISAGSSSEAILNTMSQELVPASRVALAGEYGAVEYRKSKRGPKQL
APHPNIIRVLRAFTSEVPLLPGALVDYPDVLPSRLHPEGLGHGRTLFLVM
KNYPCTLRQYLCVNTPSPRLAAMMLLQLLEGVDHLVQQGIAHRDLKSDNI
PVELDPDGCPWLVIADFGCCLADESIGLQLPFSSWYVDRGGNGCLMAPEV
STARPGPRAVIDYSKADAWAVGAIAYEIFGLVNPFYGQGKAHLESRSYQE
AQLPALPESVPPDVRQLVRALLQREASKRPSARVAANVLHLSLWGEHILA
LKNLKLDKMVGWLLQQSAATLLANRLTEKCCVETKMKMLFLANLECETLC
QAALLLCSWRAAL*

Vector Type

Bacterial

Antibiotic

Amp

Comments

Please introduce the following mutations: L369P into the existing clone DU43815 (MBP-human PINK1 T185E/S187E/T257E/S284E (residues 123-end) in pMAL4C vector)

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