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Plasmid
pET15b 6His SUMO Pink1 drosophila 182-721 + linker
Parent Plasmid
pET15b 6His SUMO1
Expressed
6HIS SUMO Pink1 drosophila 182-721 + linker
DU Number
DU46304
Genbank
Q0KHV6
Tag
6His
SUMO
Species
Drosophila Melanogaster
Sequence of Insert
View Sequence
GGATCCTCCGATACGCTAGACAGCAAGTTCACCATCGATGACTTGGAAAT
CGGTCCGCCCATAGCCAAAGGTTGTGCCGCTGTCGTCTATGCAGCGGATT
TCAAGAAAGATGTTGCCTCGGATGGTGCATCCCTGCATACCGATGCGCAG
CCGCAGGCAACGCCAGCCTTTGCGCCGAATAGCTGGAGTACCCACGAGAT
GATGTCGCCGCTGCAGAACATGTCGCGCTTTGTTCACAACTTTGGCGGCT
CTGTGGACAACGTCTTCCACTACAGCCAGCCATCTGCGGCCAGTGATTTC
GTGGGCGCCCAGTCGAGGGAACAGGACCAGCGGCACCACGAGCAACAGCA
GCATCAGAATCAGGAACAAGAGCAGCATCAGAACCAGGAGCCCAGCAGCA
GTGCCTTCAATGTGACTTCTCCAGCGAATTCAAACATCAACAGCTCTGTG
GACAGTTATCCACTGGCACTCAAAATGATGTTCAACTACGACATCCAGAG
CAACGCTCTGTCCATACTGCGTGCCATGTACAAGGAGACGGTACCGGCAC
GCCAGCGCGGCATGAATGAGGCCGCCGATGAGTGGGAACGTTTGCTGCAG
AATCAAACTGTTCACCTGCCGCGGCATCCGAACATTGTCTGTATGTTTGG
CTTCTTTTGCGACGAGGTGCGCAACTTTCCCGATGGCCATCTCCTGTATC
CGGTGGCGCAGCCGCAACGCATCAATCCACAGGGCTATGGCCGCAATATG
TCGCTGTATCTGCTGATGAAACGCTACGATCACAGTCTTCGCGGCCTGCT
CGATAGCCAGGATCTGAGCACGCGCAATCGCATCCTGCTGCTGGCTCAAA
TGCTCGAGGCTGTCAACCATTTGAGCCGTCACGGCGTTGCCCATCGTGAC
CTAAAGTCTGATAATGTGCTAATCGAGCTGCAGGACGATGCGGCGCCGGT
GCTAGTGCTCTCCGACTTTGGCTGTTGTCTGGCGGACAAGGTGCATGGCC
TGCGCCTGCCGTACGTTTCGCACGACGTCGACAAGGGCGGCAATGCGGCG
TTGATGGCGCCAGAGATCTTCAATACGATGCCCGGTCCGTTTGCCGTACT
CAATTATGGCAAGGCCGATCTGTGGGCTTGCGGTGCCCTGGCTTACGAAA
TCTTTGGCAACCGCAATCCGTTCTATTCGAGCAGCGGTGGGATGGCGCGC
GAACGCGGTGAGATGACGCTTTCGCTGAGGAACAGCGATTACCGACAGGA
CCAATTGCCGCCAATGAGCGATGCTTGCCCGCCGCTGCTGCAACAGCTGG
TCTACAACATCCTCAATCCCAACCCGTCCAAGCGGGTCAGTCCGGATATT
GCGGCAAATGTGGTGCAGCTGTTCCTCTGGGCCCCATCCAATTGGCTTAA
GGCGGGCGGCATGCCCAACAGTCCGGAGATCCTACAGTGGCTCCTTTCGC
TGACCACTAAGATTATGTGCGAGGGTCGTCCACAGATGGGCGCCGGATTG
ATGCCAGTGGCCAGCTGTGGCAATAGGCGCGCCTATGTGGAGTACCTGCT
CATATGCAGCTTTCTGGCACGCGCCCGGCTGCGTCGCATTCGCGGCGCCC
TCAACTGGATACAGAATGTGGTGGCGTAGGCGGCCGC
CGGTCCGCCCATAGCCAAAGGTTGTGCCGCTGTCGTCTATGCAGCGGATT
TCAAGAAAGATGTTGCCTCGGATGGTGCATCCCTGCATACCGATGCGCAG
CCGCAGGCAACGCCAGCCTTTGCGCCGAATAGCTGGAGTACCCACGAGAT
GATGTCGCCGCTGCAGAACATGTCGCGCTTTGTTCACAACTTTGGCGGCT
CTGTGGACAACGTCTTCCACTACAGCCAGCCATCTGCGGCCAGTGATTTC
GTGGGCGCCCAGTCGAGGGAACAGGACCAGCGGCACCACGAGCAACAGCA
GCATCAGAATCAGGAACAAGAGCAGCATCAGAACCAGGAGCCCAGCAGCA
GTGCCTTCAATGTGACTTCTCCAGCGAATTCAAACATCAACAGCTCTGTG
GACAGTTATCCACTGGCACTCAAAATGATGTTCAACTACGACATCCAGAG
CAACGCTCTGTCCATACTGCGTGCCATGTACAAGGAGACGGTACCGGCAC
GCCAGCGCGGCATGAATGAGGCCGCCGATGAGTGGGAACGTTTGCTGCAG
AATCAAACTGTTCACCTGCCGCGGCATCCGAACATTGTCTGTATGTTTGG
CTTCTTTTGCGACGAGGTGCGCAACTTTCCCGATGGCCATCTCCTGTATC
CGGTGGCGCAGCCGCAACGCATCAATCCACAGGGCTATGGCCGCAATATG
TCGCTGTATCTGCTGATGAAACGCTACGATCACAGTCTTCGCGGCCTGCT
CGATAGCCAGGATCTGAGCACGCGCAATCGCATCCTGCTGCTGGCTCAAA
TGCTCGAGGCTGTCAACCATTTGAGCCGTCACGGCGTTGCCCATCGTGAC
CTAAAGTCTGATAATGTGCTAATCGAGCTGCAGGACGATGCGGCGCCGGT
GCTAGTGCTCTCCGACTTTGGCTGTTGTCTGGCGGACAAGGTGCATGGCC
TGCGCCTGCCGTACGTTTCGCACGACGTCGACAAGGGCGGCAATGCGGCG
TTGATGGCGCCAGAGATCTTCAATACGATGCCCGGTCCGTTTGCCGTACT
CAATTATGGCAAGGCCGATCTGTGGGCTTGCGGTGCCCTGGCTTACGAAA
TCTTTGGCAACCGCAATCCGTTCTATTCGAGCAGCGGTGGGATGGCGCGC
GAACGCGGTGAGATGACGCTTTCGCTGAGGAACAGCGATTACCGACAGGA
CCAATTGCCGCCAATGAGCGATGCTTGCCCGCCGCTGCTGCAACAGCTGG
TCTACAACATCCTCAATCCCAACCCGTCCAAGCGGGTCAGTCCGGATATT
GCGGCAAATGTGGTGCAGCTGTTCCTCTGGGCCCCATCCAATTGGCTTAA
GGCGGGCGGCATGCCCAACAGTCCGGAGATCCTACAGTGGCTCCTTTCGC
TGACCACTAAGATTATGTGCGAGGGTCGTCCACAGATGGGCGCCGGATTG
ATGCCAGTGGCCAGCTGTGGCAATAGGCGCGCCTATGTGGAGTACCTGCT
CATATGCAGCTTTCTGGCACGCGCCCGGCTGCGTCGCATTCGCGGCGCCC
TCAACTGGATACAGAATGTGGTGGCGTAGGCGGCCGC
Amino Acid Sequence
View Sequence
MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTH
LKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGSSDTLDSKFTIDDLEIGPPIAKGCAAVVYAADFKKDVASDGASLHT
DAQPQATPAFAPNSWSTHEMMSPLQNMSRFVHNFGGSVDNVFHYSQPSAA
SDFVGAQSREQDQRHHEQQQHQNQEQEQHQNQEPSSSAFNVTSPANSNIN
SSVDSYPLALKMMFNYDIQSNALSILRAMYKETVPARQRGMNEAADEWER
LLQNQTVHLPRHPNIVCMFGFFCDEVRNFPDGHLLYPVAQPQRINPQGYG
RNMSLYLLMKRYDHSLRGLLDSQDLSTRNRILLLAQMLEAVNHLSRHGVA
HRDLKSDNVLIELQDDAAPVLVLSDFGCCLADKVHGLRLPYVSHDVDKGG
NAALMAPEIFNTMPGPFAVLNYGKADLWACGALAYEIFGNRNPFYSSSGG
MARERGEMTLSLRNSDYRQDQLPPMSDACPPLLQQLVYNILNPNPSKRVS
PDIAANVVQLFLWAPSNWLKAGGMPNSPEILQWLLSLTTKIMCEGRPQMG
AGLMPVASCGNRRAYVEYLLICSFLARARLRRIRGALNWIQNVVA*A
LKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGSSDTLDSKFTIDDLEIGPPIAKGCAAVVYAADFKKDVASDGASLHT
DAQPQATPAFAPNSWSTHEMMSPLQNMSRFVHNFGGSVDNVFHYSQPSAA
SDFVGAQSREQDQRHHEQQQHQNQEQEQHQNQEPSSSAFNVTSPANSNIN
SSVDSYPLALKMMFNYDIQSNALSILRAMYKETVPARQRGMNEAADEWER
LLQNQTVHLPRHPNIVCMFGFFCDEVRNFPDGHLLYPVAQPQRINPQGYG
RNMSLYLLMKRYDHSLRGLLDSQDLSTRNRILLLAQMLEAVNHLSRHGVA
HRDLKSDNVLIELQDDAAPVLVLSDFGCCLADKVHGLRLPYVSHDVDKGG
NAALMAPEIFNTMPGPFAVLNYGKADLWACGALAYEIFGNRNPFYSSSGG
MARERGEMTLSLRNSDYRQDQLPPMSDACPPLLQQLVYNILNPNPSKRVS
PDIAANVVQLFLWAPSNWLKAGGMPNSPEILQWLLSLTTKIMCEGRPQMG
AGLMPVASCGNRRAYVEYLLICSFLARARLRRIRGALNWIQNVVA*A
Vector Type
Bacterial
Antibiotic
Amp
100042 R 1000 1001 1047 19007
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