PINK1 | LRRK2 Resource

Synonyms

Park6

Plasmid

pcDNA5FRTTO PINK FLAG S73A T185A S187A T257A S284A

Parent Plasmid

pcDNA5 FRT/TO

Expressed

PINK FLAG S73A T185A S187A T257A S284A

DU Number

DU46272

Genbank

Q9BXM7, EAW94935

Tag

FLAG

Species

Human

Sequence of Insert

View Sequence

GGATCCGCCACCATGGCGGTGCGACAGGCGCTGGGCCGCGGCCTGCAGCT
GGGTCGAGCGCTGCTGCTGCGCTTCACGGGCAAGCCCGGCCGGGCCTACG
GCTTGGGGCGGCCGGGCCCGGCGGCGGGCTGTGTCCGCGGGGAGCGTCCA
GGCTGGGCCGCAGGACCGGGCGCGGAGCCTCGCAGGGTCGGGCTCGGGCT
CCCTAACCGTCTCCGCTTCTTCCGCCAGGCGGTGGCCGGGCTGGCGGCGC
GGTTGCAGCGGCAGTTCGTGGTGCGGGCCTGGGGCTGCGCGGGCCCTTGC
GGCCGGGCAGTCTTTCTGGCCTTCGGGCTAGGGCTGGGCCTCATCGAGGA
AAAACAGGCGGAGAGCCGGCGGGCGGTCTCGGCCTGTCAGGAGATCCAGG
CAATTTTTACCCAGAAAAGCAAGCCGGGGCCTGACCCGTTGGACACGAGA
CGCTTGCAGGGCTTTCGGCTGGAGGAGTATCTGATAGGGCAGTCCATTGG
TAAGGGCTGCAGTGCTGCTGTGTATGAAGCCACCATGCCTACATTGCCCC
AGAACCTGGAGGTGGCAAAGGCCACCGGGTTGCTTCCAGGGAGAGGCCCA
GGTACCAGTGCACCAGGAGAAGGGCAGGAGCGAGCTCCGGGGGCCCCTGC
CTTCCCCTTGGCCATCAAGATGATGTGGAACATCTCGGCAGGTTCCTCCA
GCGAAGCCATCTTGAACACAATGAGCCAGGAGCTGGTCCCAGCGAGCCGA
GTGGCCTTGGCTGGGGAGTATGGAGCAGTCGCTTACAGAAAATCCAAGAG
AGGTCCCAAGCAACTAGCCCCTCACCCCAACATCATCCGGGTTCTCCGCG
CCTTCACCTCTGCCGTGCCGCTGCTGCCAGGGGCCCTGGTCGACTACCCT
GATGTGCTGCCCTCACGCCTCCACCCTGAAGGCCTGGGCCATGGCCGGAC
GCTGTTCCTCGTTATGAAGAACTATCCCTGTACCCTGCGCCAGTACCTTT
GTGTGAACACACCCAGCCCCCGCCTCGCCGCCATGATGCTGCTGCAGCTG
CTGGAAGGCGTGGACCATCTGGTTCAACAGGGCATCGCGCACAGAGACCT
GAAATCCGACAACATCCTTGTGGAGCTGGACCCAGACGGCTGCCCCTGGC
TGGTGATCGCAGATTTTGGCTGCTGCCTGGCTGATGAGAGCATCGGCCTG
CAGTTGCCCTTCAGCAGCTGGTACGTGGATCGGGGCGGAAACGGCTGTCT
GATGGCCCCAGAGGTGTCCACGGCCCGTCCTGGCCCCAGGGCAGTGATTG
ACTACAGCAAGGCTGATGCCTGGGCAGTGGGAGCCATCGCCTATGAAATC
TTCGGGCTTGTCAATCCCTTCTACGGCCAGGGCAAGGCCCACCTTGAAAG
CCGCAGCTACCAAGAGGCTCAGCTACCTGCACTGCCCGAGTCAGTGCCTC
CAGACGTGAGACAGTTGGTGAGGGCACTGCTCCAGCGAGAGGCCAGCAAG
AGACCATCTGCCCGAGTAGCCGCAAATGTGCTTCATCTAAGCCTCTGGGG
TGAACATATTCTAGCCCTGAAGAATCTGAAGTTAGACAAGATGGTTGGCT
GGCTCCTCCAACAATCGGCCGCCACTTTGTTGGCCAACAGGCTCACAGAG
AAGTGTTGTGTGGAAACAAAAATGAAGATGCTCTTTCTGGCTAACCTGGA
GTGTGAAACGCTCTGCCAGGCAGCCCTCCTCCTCTGCTCATGGAGGGCAG
CCCTGGACTACAAGGACGACGATGACAAGTAGCGGCCGC

Amino Acid Sequence

View Sequence

MAVRQALGRGLQLGRALLLRFTGKPGRAYGLGRPGPAAGCVRGERPGWAA
GPGAEPRRVGLGLPNRLRFFRQAVAGLAARLQRQFVVRAWGCAGPCGRAV
FLAFGLGLGLIEEKQAESRRAVSACQEIQAIFTQKSKPGPDPLDTRRLQG
FRLEEYLIGQSIGKGCSAAVYEATMPTLPQNLEVAKATGLLPGRGPGTSA
PGEGQERAPGAPAFPLAIKMMWNISAGSSSEAILNTMSQELVPASRVALA
GEYGAVAYRKSKRGPKQLAPHPNIIRVLRAFTSAVPLLPGALVDYPDVLP
SRLHPEGLGHGRTLFLVMKNYPCTLRQYLCVNTPSPRLAAMMLLQLLEGV
DHLVQQGIAHRDLKSDNILVELDPDGCPWLVIADFGCCLADESIGLQLPF
SSWYVDRGGNGCLMAPEVSTARPGPRAVIDYSKADAWAVGAIAYEIFGLV
NPFYGQGKAHLESRSYQEAQLPALPESVPPDVRQLVRALLQREASKRPSA
RVAANVLHLSLWGEHILALKNLKLDKMVGWLLQQSAATLLANRLTEKCCV
ETKMKMLFLANLECETLCQAALLLCSWRAALDYKDDDDK*

Vector Type

Mammalian

Antibiotic

Amp

Comments

Please introduce S73A mutation into the existing clone DU43236 (PINK1 T185A/S187A/T257A/S284A-FLAG in pcDNA5FRTTO vector)

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