PINK1 (C.elegans) | LRRK2 Resource

Plasmid

pET6HIS SUMO Pink1 C elegans 129-641 + linker

Parent Plasmid

pET15b 6His SUMO1

Expressed

6HIS SUMO Pink1 c elegans129-641 + linker

DU Number

DU46164

Genbank

NP_495017

Tag

6His

SUMO

Species

Caenorhabditis elegans

Sequence of Insert

View Sequence

ggatccGAATGGCCGAATAGAATTGATTCTTATGAGTTTGGGGAATTTCT CGGTCAAGGATGCAATGCAGCAGTTTACTCTGCGAGATTAGCCAATTCTG ATGCAGAATCCTCAGGGAATACTCACTATGGTGCAGGGTTTAATGAAGTC ACAAATATACTTGCAGAAATTCCGCCAGTTAGCAAAGTTGCACAAAAGAA ATTCCCGTTGGCAATCAAATTAATGTTTAATTTTGAACATGATCGCGATG GAGATGCTCATCTCTTGAAATCAATGGGAAATGAATTGGCTCCATATCCG AATGCTGCAAAGTTGCTCAATGGACAAATGGGAACATTTAGACCTCTTCC AGCAAAACATCCAAATGTTGTTCGAATTCAGACAGCTTTTATTGATTCGT TAAAAGTTTTGCCAGATGCGATTGAACGATATCCAGATGCCCTTCACACT GCACGTTGGTATGAGTCAATTGCCTCCGAACCGAAAACAATGTACGTAGT AATGAGACGATACCGACAAACACTTCATGAATATGTATGGACTCGTCATC GAAATTATTGGACAGGACGAGTGATAATTGCTCAACTATTAGAAGCATGT ACATATCTTCATAAGCATAAAGTTGCTCAGCGAGACATGAAAAGTGATAA TATTCTTCTGGAATATGATTTTGACGACGAGATTCCCCAATTAGTTGTCG CCGATTTTGGATGTGCACTTGCATGTGACAATTGGCAAGTAGACTATGAA TCAGATGAAGTTAGTCTTGGAGGAAATGCCAAGACAAAAGCACCAGAAAT TGCGACGGCTGTTCCTGGAAAGAATGTAAAAGTAAACTTCGAAATGGCAG ATACATGGGCAGCTGGAGGCCTTTCTTATGAAGTTCTAACACGATCAAAT CCATTCTACAAACTTCTTGATACTGCAACATACCAGGAATCAGAACTACC AGCACTCCCATCTCGTGTCAATTTTGTGGCACGAGATGTCATTTTTGACC TACTCAAGCGAGATCCTAATGAAAGAGTCAAGCCGAATATTGCTGCAAAT GCGTTGAATTTGTCATTGTTCAGAATGGGAGAAGATGTGAAGCAGATGAT GGAAAAATGTGGAATATCTCAAATGACTACTCTATTGGCTGGAAGTTCTA AAGTTTTGAGTCAAAAAATCAATAGTCGTCTGGACAAAGTGATGAATCTG ATTACTGCTGAAACTATCATGGCCAACCTAGCTCCACATTTGATTAGTCG AGCAGAACGACAACTTCGAGCAACATTTCTTTCAAGAATGAATCGAGAAG ATATTTGGAGAAGTCTTCAATATTTCTTCCCAGCTGGTGTTCAACTTGAC ACACCTGCCACATCATCAGACTGTTTGGAGACTATTTCCAGTTTGATGTC GAGTTTTTCAAATGATTCAGAAAATTACGAGAAGCAACAGAAACCGGCTA AAAATGGATACAACAATGTTCCACTTCTTCTCAGAAATGTTATCCGTACA GATGCGGATGGAATCAATGGAATTGTACATAGAGTTCGATCTAAATAGgc ggccgc

Amino Acid Sequence

View Sequence

MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTH
LKKLKESYCQRQGVPMNSLRFLFEGQRIADNHSPKELGMEEEDVIEVYQE
QTGGSEWPNRIDSYEFGEFLGQGCNAAVYSARLANSDAESSGNTHYGAGF
NEVTNILAEIPPVSKVAQKKFPLAIKLMFNFEHDRDGDAHLLKSMGNELA
PYPNAAKLLNGQMGTFRPLPAKHPNVVRIQTAFIDSLKVLPDAIERYPDA
LHTARWYESIASEPKTMYVVMRRYRQTLHEYVWTRHRNYWTGRVIIAQLL
EACTYLHKHKVAQRDMKSDNILLEYDFDDEIPQLVVADFGCALACDNWQV
DYESDEVSLGGNAKTKAPEIATAVPGKNVKVNFEMADTWAAGGLSYEVLT
RSNPFYKLLDTATYQESELPALPSRVNFVARDVIFDLLKRDPNERVKPNI
AANALNLSLFRMGEDVKQMMEKCGISQMTTLLAGSSKVLSQKINSRLDKV
MNLITAETIMANLAPHLISRAERQLRATFLSRMNREDIWRSLQYFFPAGV
QLDTPATSSDCLETISSLMSSFSNDSENYEKQQKPAKNGYNNVPLLLRNV
IRTDADGINGIVHRVRSK*

Vector Type

Bacterial

Antibiotic

Amp

100042 R 1000 1001 1047 19007