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Plasmid
pET6HIS SUMO pink1 Acyrthosiphon 146-578 + linker
Parent Plasmid
pET15b 6His SUMO1
Expressed
His-SUMO-Acyrthosiphon PINK1 kinase-dead [residues 146-end] [D370A]
DU Number
DU46135
Genbank
XM_001946909
Tag
6His
SUMO
Species
Acyrthosiphon pisum
Sequence of Insert
View Sequence
ggatccctgaaattaaattcatctttgggtttgaagcagtttgaattagg
atctgttattgctaaaggaaataatgctgttgtatacgaagcaagaaaaa
ttgaaaaggacagagacccaagtgacgtaatcaagcaagggtcatcaaat
acaatgtatcctttggctgtgaaaatgatgtttaattatgatgctgaaag
taatgccatgtcaatattacgtgccatgagtagagaaacggttccatctc
gtagtgtgcaacatgatttttctagatcagatcagcaatggcctattggt
tatgatcaaaatttaattaaattatcaccacacccaaacattgttatgat
gcatttgtgctttgcggattacgtgccagagttgattgattcgcatacat
tatatcctcaagctctaccaactagaataaatccagaaggttatggtcgg
aacatgagtctatttctggtgatgaaaaagtatgacatgagtttgaaaca
gtatttatctaaagctccatcactgaaaattagagataaaattcttctgc
tcgctcaattattggaagctgtttctcatttgtctaatcaaaacgtggca
catagggatatgaagactgataatatcttattagatgtatccgaaggtaa
tgatgtatgtcctgcattagtcattacagattttgggtgttgtcttgctg
ataaggataatggacttaatcttccatataaatctgctgacactgatcgt
ggaggaaatatagcgctgatggcaccagaagttgtcactgctcgaccagg
aacatttggatatatagattattcaaaagcagatgtatgggctgttggtg
ctatggcatatgaaatattttctgataataatccattttatagttatgaa
agaggtaaacctccattgttgcgcaattctacttacaaggaagaagatct
tcctgcatttagtagtgacataccattaattatacaaacattaattcact
cactattgcagagatcaacatctaagagattagatgcatcatttgctgct
acggtttgtcagatatatctatgggcaccatcttcatggattacaaaata
tgtgatgccaactagtaatgaggtgttgcaatggatgttgtgtttaacaa
ccaaggtgttatgtgaaggaggaacaatgatgtcaacttcaagcactaaa
caacgtacctgtacagaatatcaattgatcactacatttttacatagagc
aaaattgtatgaaataaaaaatgcgttatattggatgcattcaatacaac
aagaataagcggccgc
atctgttattgctaaaggaaataatgctgttgtatacgaagcaagaaaaa
ttgaaaaggacagagacccaagtgacgtaatcaagcaagggtcatcaaat
acaatgtatcctttggctgtgaaaatgatgtttaattatgatgctgaaag
taatgccatgtcaatattacgtgccatgagtagagaaacggttccatctc
gtagtgtgcaacatgatttttctagatcagatcagcaatggcctattggt
tatgatcaaaatttaattaaattatcaccacacccaaacattgttatgat
gcatttgtgctttgcggattacgtgccagagttgattgattcgcatacat
tatatcctcaagctctaccaactagaataaatccagaaggttatggtcgg
aacatgagtctatttctggtgatgaaaaagtatgacatgagtttgaaaca
gtatttatctaaagctccatcactgaaaattagagataaaattcttctgc
tcgctcaattattggaagctgtttctcatttgtctaatcaaaacgtggca
catagggatatgaagactgataatatcttattagatgtatccgaaggtaa
tgatgtatgtcctgcattagtcattacagattttgggtgttgtcttgctg
ataaggataatggacttaatcttccatataaatctgctgacactgatcgt
ggaggaaatatagcgctgatggcaccagaagttgtcactgctcgaccagg
aacatttggatatatagattattcaaaagcagatgtatgggctgttggtg
ctatggcatatgaaatattttctgataataatccattttatagttatgaa
agaggtaaacctccattgttgcgcaattctacttacaaggaagaagatct
tcctgcatttagtagtgacataccattaattatacaaacattaattcact
cactattgcagagatcaacatctaagagattagatgcatcatttgctgct
acggtttgtcagatatatctatgggcaccatcttcatggattacaaaata
tgtgatgccaactagtaatgaggtgttgcaatggatgttgtgtttaacaa
ccaaggtgttatgtgaaggaggaacaatgatgtcaacttcaagcactaaa
caacgtacctgtacagaatatcaattgatcactacatttttacatagagc
aaaattgtatgaaataaaaaatgcgttatattggatgcattcaatacaac
aagaataagcggccgc
Amino Acid Sequence
View Sequence
MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTH
LKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGSLKLNSSLGLKQFELGSVIAKGNNAVVYEARKIEKDRDPSDVIKQG
SSNTMYPLAVKMMFNYDAESNAMSILRAMSRETVPSRSVQHDFSRSDQQW
PIGYDQNLIKLSPHPNIVMMHLCFADYVPELIDSHTLYPQALPTRINPEG
YGRNMSLFLVMKKYDMSLKQYLSKAPSLKIRDKILLLAQLLEAVSHLSNQ
NVAHRDMKTDNILLDVSEGNDVCPALVITDFGCCLADKDNGLNLPYKSAD
TDRGGNIALMAPEVVTARPGTFGYIDYSKADVWAVGAMAYEIFSDNNPFY
SYERGKPPLLRNSTYKEEDLPAFSSDIPLIIQTLIHSLLQRSTSKRLDAS
FAATVCQIYLWAPSSWITKYVMPTSNEVLQWMLCLTTKVLCEGGTMMSTS
STKQRTCTEYQLITTFLHRAKLYEIKNALYWMHSIQQE*
LKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGSLKLNSSLGLKQFELGSVIAKGNNAVVYEARKIEKDRDPSDVIKQG
SSNTMYPLAVKMMFNYDAESNAMSILRAMSRETVPSRSVQHDFSRSDQQW
PIGYDQNLIKLSPHPNIVMMHLCFADYVPELIDSHTLYPQALPTRINPEG
YGRNMSLFLVMKKYDMSLKQYLSKAPSLKIRDKILLLAQLLEAVSHLSNQ
NVAHRDMKTDNILLDVSEGNDVCPALVITDFGCCLADKDNGLNLPYKSAD
TDRGGNIALMAPEVVTARPGTFGYIDYSKADVWAVGAMAYEIFSDNNPFY
SYERGKPPLLRNSTYKEEDLPAFSSDIPLIIQTLIHSLLQRSTSKRLDAS
FAATVCQIYLWAPSSWITKYVMPTSNEVLQWMLCLTTKVLCEGGTMMSTS
STKQRTCTEYQLITTFLHRAKLYEIKNALYWMHSIQQE*
Vector Type
Bacterial
Antibiotic
Amp
Comments
in pET vector
100042 R 1000 1001 1047 19007
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