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Plasmid
pET6HIS SUMO pink1 rat 144-580 + linker
Parent Plasmid
pET15b 6His SUMO1
Expressed
His-SUMO-Rat PINK1 [residues 144-end]
DU Number
DU46134
Genbank
B5DFG1
Tag
6His
SUMO
Species
Rat
Sequence of Insert
View Sequence
agatctgacacacgacgttggcagggcttccgcctggaggattatctgat
aggacaggccatcggcaagggctgcaatgccgctgtgtatgaagccacca
tgcccacactgccccagcacctggagaaggccaaacaccttggccttcta
ggaaaaggcccagatgtcgtctcaaagggagcagatggggagcaggctcc
aggggcccccgccttcccctttgccatcaaaatgatgtggaatatctcgg
caggatcctccagcgaagccatcttaagcaaaatgagccaggagctggtc
ccggcaagccgcatggctttggatggagagtatggagccgttacttacag
aagatccagagacggtcccaagcagcttgccccgcaccccaacatcatcc
gggttttccgcgccttcacttcatctgtgcccctcctgcccggggccctg
gctgactatcctgacatgctacccccacactactacccagaaggcctggg
ccacggccgcacactcttcctcgttatgaagaactacccctgtaccctgc
gccagtaccttgaagagcaaactcccagctcccgcctggctaccatgatg
accttgcagttactggagggcgtggaccacctggttcagcagggcattgc
ccatcgagacctcaagtccgacaacatacttgtggaatgggactcagatg
gctgcccctggctggtgatctccgactttggctgctgcctggctgatgag
cgtgttggcctgcagttacctttcaacagctccagtgtagagcgtggtgg
caatggctccctcatggcccctgaggtgtccacagcccattctggccccc
atgcagtaattgactacagcaaggctgacacgtgggctgtgggggccatc
gcctatgaaatcttcggccttgccaaccccttctatggccaaggcagtgc
ccaccttgagagccgcagctaccaggaagctcagctgcccgagatgccca
agtcggtgcctccagaaacaagacagctggtgaggtccctgctccagcga
gaggccaacaagaggccgtctgcgcgcatagctgccaacgtgctgcactt
aagcctctggggtgagcatcttctagccctgaagaatctgaaattggaca
agatgattgcctggctcctccagcagtcagcagccactctgctggctgac
aggctgagagagaagagctgcgtggagacgaagctgcagatgctgttcct
ggctaacctggagtgtgaggcactctgccaggcagccctcctcctctctt
cctggagggcagccccatgagcggcgc
aggacaggccatcggcaagggctgcaatgccgctgtgtatgaagccacca
tgcccacactgccccagcacctggagaaggccaaacaccttggccttcta
ggaaaaggcccagatgtcgtctcaaagggagcagatggggagcaggctcc
aggggcccccgccttcccctttgccatcaaaatgatgtggaatatctcgg
caggatcctccagcgaagccatcttaagcaaaatgagccaggagctggtc
ccggcaagccgcatggctttggatggagagtatggagccgttacttacag
aagatccagagacggtcccaagcagcttgccccgcaccccaacatcatcc
gggttttccgcgccttcacttcatctgtgcccctcctgcccggggccctg
gctgactatcctgacatgctacccccacactactacccagaaggcctggg
ccacggccgcacactcttcctcgttatgaagaactacccctgtaccctgc
gccagtaccttgaagagcaaactcccagctcccgcctggctaccatgatg
accttgcagttactggagggcgtggaccacctggttcagcagggcattgc
ccatcgagacctcaagtccgacaacatacttgtggaatgggactcagatg
gctgcccctggctggtgatctccgactttggctgctgcctggctgatgag
cgtgttggcctgcagttacctttcaacagctccagtgtagagcgtggtgg
caatggctccctcatggcccctgaggtgtccacagcccattctggccccc
atgcagtaattgactacagcaaggctgacacgtgggctgtgggggccatc
gcctatgaaatcttcggccttgccaaccccttctatggccaaggcagtgc
ccaccttgagagccgcagctaccaggaagctcagctgcccgagatgccca
agtcggtgcctccagaaacaagacagctggtgaggtccctgctccagcga
gaggccaacaagaggccgtctgcgcgcatagctgccaacgtgctgcactt
aagcctctggggtgagcatcttctagccctgaagaatctgaaattggaca
agatgattgcctggctcctccagcagtcagcagccactctgctggctgac
aggctgagagagaagagctgcgtggagacgaagctgcagatgctgttcct
ggctaacctggagtgtgaggcactctgccaggcagccctcctcctctctt
cctggagggcagccccatgagcggcgc
Amino Acid Sequence
View Sequence
MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTH
LKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGSDTRRWQGFRLEDYLIGQAIGKGCNAAVYEATMPTLPQHLEKAKHL
GLLGKGPDVVSKGADGEQAPGAPAFPFAIKMMWNISAGSSSEAILSKMSQ
ELVPASRMALDGEYGAVTYRRSRDGPKQLAPHPNIIRVFRAFTSSVPLLP
GALADYPDMLPPHYYPEGLGHGRTLFLVMKNYPCTLRQYLEEQTPSSRLA
TMMTLQLLEGVDHLVQQGIAHRDLKSDNILVEWDSDGCPWLVISDFGCCL
ADERVGLQLPFNSSSVERGGNGSLMAPEVSTAHSGPHAVIDYSKADTWAV
GAIAYEIFGLANPFYGQGSAHLESRSYQEAQLPEMPKSVPPETRQLVRSL
LQREANKRPSARIAANVLHLSLWGEHLLALKNLKLDKMIAWLLQQSAATL
LADRLREKSCVETKLQMLFLANLECEALCQAALLLSSWRAAP*
LKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGSDTRRWQGFRLEDYLIGQAIGKGCNAAVYEATMPTLPQHLEKAKHL
GLLGKGPDVVSKGADGEQAPGAPAFPFAIKMMWNISAGSSSEAILSKMSQ
ELVPASRMALDGEYGAVTYRRSRDGPKQLAPHPNIIRVFRAFTSSVPLLP
GALADYPDMLPPHYYPEGLGHGRTLFLVMKNYPCTLRQYLEEQTPSSRLA
TMMTLQLLEGVDHLVQQGIAHRDLKSDNILVEWDSDGCPWLVISDFGCCL
ADERVGLQLPFNSSSVERGGNGSLMAPEVSTAHSGPHAVIDYSKADTWAV
GAIAYEIFGLANPFYGQGSAHLESRSYQEAQLPEMPKSVPPETRQLVRSL
LQREANKRPSARIAANVLHLSLWGEHLLALKNLKLDKMIAWLLQQSAATL
LADRLREKSCVETKLQMLFLANLECEALCQAALLLSSWRAAP*
Vector Type
Bacterial
Antibiotic
Amp
Comments
in pET vector
100042 R 1000 1001 1047 19007
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