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Plasmid
pET6HIS SUMO PINK1 Nasonia 153-614 D382A
Parent Plasmid
pET15b 6His SUMO1
Expressed
His-SUMO-Nasonia-PINK1 kinase dead [residues 153-end] [D382A]
DU Number
DU46166
Genbank
XM_001605929
Tag
6His
SUMO
Species
Nasonia vitripennis
Amino Acid Sequence
View Sequence
MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTH
LKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGNYETTLTDDNQISLQNFVIGPLIAKGCSAVVYAARTKNSDDNQNSE
EITIDRSSNITSFPLAIKMMFNYDAESNATAILRAMFRETVPAWKHYKNE
ELSHWEQKMADNKKTLPAHSNIVAMYSVFADRVPFLPGSIKMYPDALPAR
LNPEGSGRNMSLFLLMKRYDVTLKQYVSDRQLNTRESILLFAQLLEGVAH
MNSHGIAHRDLKADNILLDISEESDNCPTLVITAFGCCLADKNHGLYLPY
NTHDTDKGGNAALMAPEIVTAEPGPFTSLNYTKSDLWTVGTIAYEIFGMK
NPFYGSVKEKAMLKNYDYKVEDLPPLPETVPEIISALVRNILTRSTYKRL
SVEMAATIMQLFLWAPSSWLQKDSKIPSSSEILQWLLCLTTKVLCEGRND
SLEERNSIDAQKTESPHQLSYRRSLSTRSCGRRTMPEYQLIASFLGRVSL
FNIREGFKWIQRNNYV*
LKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGNYETTLTDDNQISLQNFVIGPLIAKGCSAVVYAARTKNSDDNQNSE
EITIDRSSNITSFPLAIKMMFNYDAESNATAILRAMFRETVPAWKHYKNE
ELSHWEQKMADNKKTLPAHSNIVAMYSVFADRVPFLPGSIKMYPDALPAR
LNPEGSGRNMSLFLLMKRYDVTLKQYVSDRQLNTRESILLFAQLLEGVAH
MNSHGIAHRDLKADNILLDISEESDNCPTLVITAFGCCLADKNHGLYLPY
NTHDTDKGGNAALMAPEIVTAEPGPFTSLNYTKSDLWTVGTIAYEIFGMK
NPFYGSVKEKAMLKNYDYKVEDLPPLPETVPEIISALVRNILTRSTYKRL
SVEMAATIMQLFLWAPSSWLQKDSKIPSSSEILQWLLCLTTKVLCEGRND
SLEERNSIDAQKTESPHQLSYRRSLSTRSCGRRTMPEYQLIASFLGRVSL
FNIREGFKWIQRNNYV*
Vector Type
Bacterial
Antibiotic
Amp
Comments
in pET vector
100042 R 1000 1001 1047 19007
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