PINK1 | LRRK2 Resource

Plasmid

pMAL4C Pink1 123-581 T185E S187E T257E S284E G309D

Parent Plasmid

pMAL4C

Expressed

MBP-Pink1 123-581 T185E S187E T257E S284E G309D

DU Number

DU46116

Genbank

Q9BXM7

Tag

MBP

Species

Human

Sequence of Insert

View Sequence

GGATCCGCCACCTCGGCCTGTCAGGAGATCCAGGCAATTTTTACCCAGAA
AAGCAAGCCGGGGCCTGACCCGTTGGACACGAGACGCTTGCAGGGCTTTA
GGCTGGAGGAGTATCTGATAGGGCAGTCCATTGGTAAGGGCTGCAGTGCT
GCTGTGTATGAAGCCACCATGCCTACATTGCCCCAGAACCTGGAGGTGGA
AAAGGAAACCGGGTTGCTTCCAGGGAGAGGCCCAGGTACCAGTGCACCAG
GAGAAGGGCAGGAGCGAGCTCCGGGGGCCCCTGCCTTCCCCTTGGCCATC
AAGATGATGTGGAACATCTCGGCAGGTTCCTCCAGCGAAGCCATCTTGAA
CACAATGAGCCAGGAGCTGGTCCCAGCGAGCCGAGTGGCCTTGGCTGGGG
AGTATGGAGCAGTCGAATACAGAAAATCCAAGAGAGGTCCCAAGCAACTA
GCCCCTCACCCCAACATCATCCGGGTTCTCCGCGCCTTCACCTCTGAAGT
GCCGCTGCTGCCAGGGGCCCTGGTCGACTACCCTGATGTGCTGCCCTCAC
GCCTCCACCCTGAAGGCCTGGACCATGGCCGGACGCTGTTCCTCGTTATG
AAGAACTATCCCTGTACCCTGCGCCAGTACCTTTGTGTGAACACACCCAG
CCCCCGCCTCGCCGCCATGATGCTGCTGCAGCTGCTGGAAGGCGTGGACC
ATCTGGTTCAACAGGGCATCGCGCACAGAGACCTGAAATCCGACAACATC
CTTGTGGAGCTGGACCCAGACGGCTGCCCCTGGCTGGTGATCGCAGATTT
TGGCTGCTGCCTGGCTGATGAGAGCATCGGCCTGCAGTTGCCCTTCAGCA
GCTGGTACGTGGATCGGGGCGGAAACGGCTGTCTGATGGCCCCAGAGGTG
TCCACGGCCCGTCCTGGCCCCAGGGCAGTGATTGACTACAGCAAGGCTGA
TGCCTGGGCAGTGGGAGCCATCGCCTATGAAATCTTCGGGCTTGTCAATC
CCTTCTACGGCCAGGGCAAGGCCCACCTTGAAAGCCGCAGCTACCAAGAG
GCTCAGCTACCTGCACTGCCCGAGTCAGTGCCTCCAGACGTGAGACAGTT
GGTGAGGGCACTGCTCCAGCGAGAGGCCAGCAAGAGACCATCTGCCCGAG
TAGCCGCAAATGTGCTTCATCTAAGCCTCTGGGGTGAACATATTCTAGCC
CTGAAGAATCTGAAGTTAGACAAGATGGTTGGCTGGCTCCTCCAACAATC
GGCCGCCACTTTGTTGGCCAACAGGCTCACAGAGAAGTGTTGTGTGGAAA
CAAAAATGAAGATGCTCTTTCTGGCTAACCTGGAGTGTGAAACGCTCTGC
CAGGCAGCCCTCCTCCTCTGCTCATGGAGGGCAGCCCTGTGATACAAGGA
CGACGATGACAAGTAGCGGCCGC

Amino Acid Sequence

View Sequence

MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQ
VAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRY
NGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMF
NLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLI
KNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPT
FKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPL
GAVALKSYEEELVKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVIN
AASGRQTVDEALKDAQTNSSSNNNNNNNNNNLGDDDDKVPEFLEVLFQGP
GSATSACQEIQAIFTQKSKPGPDPLDTRRLQGFRLEEYLIGQSIGKGCSA
AVYEATMPTLPQNLEVEKETGLLPGRGPGTSAPGEGQERAPGAPAFPLAI
KMMWNISAGSSSEAILNTMSQELVPASRVALAGEYGAVEYRKSKRGPKQL
APHPNIIRVLRAFTSEVPLLPGALVDYPDVLPSRLHPEGLDHGRTLFLVM
KNYPCTLRQYLCVNTPSPRLAAMMLLQLLEGVDHLVQQGIAHRDLKSDNI
LVELDPDGCPWLVIADFGCCLADESIGLQLPFSSWYVDRGGNGCLMAPEV
STARPGPRAVIDYSKADAWAVGAIAYEIFGLVNPFYGQGKAHLESRSYQE
AQLPALPESVPPDVRQLVRALLQREASKRPSARVAANVLHLSLWGEHILA
LKNLKLDKMVGWLLQQSAATLLANRLTEKCCVETKMKMLFLANLECETLC
QAALLLCSWRAAL*

Vector Type

Bacterial

Antibiotic

Amp

Comments

Mutate G309D into existing plasmid DU43815

100042 R 1000 1001 1047 19007