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Plasmid
pET6HIS SUMO Pink1 trichoplax 162-558 + linker (Codon optimized)
Parent Plasmid
pET15b 6His SUMO1
Expressed
His-SUMO-Trichoplax PINK1 162-558 + linker
DU Number
DU43764
Genbank
XP_002109370.1
Tag
SUMO
Species
Trichoplax adhaerens
Sequence of Insert
View Sequence
GGATCCGACACCACGAATTCATATAAAGTCGGCCCGCGTCTGGGTTCTCG
CAGTTGCTGTTCTGCGGTGTACGCCACCAAATGCAGTTGTGGCAAAGAAT
ACGCGATGAAAATGATGTTCAACTACGGTAACTCTAAAGATGACTTCATC
CGCAAAAACTTCCTGCGTGAATATTGCCTGCTGAGTCCGCTGCCGGTGAC
GCGTGTTGATGAAATGGAAAGCTCTAAAAACTCCCAGTCACGCATTCTGG
TGCACCCGAACATCATCCGTCTGGATAAAGTTATCATCGACGAAATCCAA
TACTGTGATGACGCAATGCATGAATACCCGGATGCTCTGCCGCCGTCGCC
GCTGAATCCGAGCGGTCTGGGTCGTCACCGTACCATGTTCCTGTTCATGA
AAAAATACGACATGACGCTGCGCGAATACGTTCATTCCAAAGGCATTATC
CCGAACGATGAAGCAACCATGCTGCTGCTGCAGCTGCTGCAGGGTGTGGA
CTATCTGAACAAAAATTACATTGCTCACCGTGATCTGAAAAGCGACAATA
TTCTGATCGAAGAAGTTGATCCGTATCCGCTGCTGGCGATCACCGACTTC
GGCGAATGCCTGGATGACGCAGCTCTGAAACTGGTTGTCCCGTACATTAG
CAACTGTATCGATCGCGGCGGTAATAGTTCCCTGATGGCACCGGAAATTA
AAAACGCTGAACCGGGTCATAATTCTGTTCTGGATTATCGTCGCAGTGAC
GTCTGGGCGATTGGCTGCCTGGCCTTTGAAATCTTCGGTCAGGAAAACCC
GTTTCTGAATACCTTCGATTCATCGAGCTATCAAGAATACGAACTGCCGG
AACTGCCGTCTCGTGTCCCGCTGATTGTGCGTAAAATCGTTCGCAACCTG
CTGCGTAAAGATTCTAGTAATCGTCTGCCGGCATCCCTGGCTATTGCAGC
ACTGTCATTTTGGCGTTGGGGTCCGATCAATTGGCGTGCACTGATGATGT
ATGAAACCGGTCTGAGTAACCTGAATGAAGAAGATATTCAGCGTTGGCTG
TCGGAAACGGCCTTCCAGCTGAGCATTCAAATCATGCAGTGTCAAGCGCA
TGGTCACAAACTGACCCCGGAACTGCAGGCCAAATACCATTTTCTGGTCT
CAACGAGTGCCGCTGACATCAAACTGGCTGTGGAATCCCTGTATTGCTAA
GCGGCCGC
CAGTTGCTGTTCTGCGGTGTACGCCACCAAATGCAGTTGTGGCAAAGAAT
ACGCGATGAAAATGATGTTCAACTACGGTAACTCTAAAGATGACTTCATC
CGCAAAAACTTCCTGCGTGAATATTGCCTGCTGAGTCCGCTGCCGGTGAC
GCGTGTTGATGAAATGGAAAGCTCTAAAAACTCCCAGTCACGCATTCTGG
TGCACCCGAACATCATCCGTCTGGATAAAGTTATCATCGACGAAATCCAA
TACTGTGATGACGCAATGCATGAATACCCGGATGCTCTGCCGCCGTCGCC
GCTGAATCCGAGCGGTCTGGGTCGTCACCGTACCATGTTCCTGTTCATGA
AAAAATACGACATGACGCTGCGCGAATACGTTCATTCCAAAGGCATTATC
CCGAACGATGAAGCAACCATGCTGCTGCTGCAGCTGCTGCAGGGTGTGGA
CTATCTGAACAAAAATTACATTGCTCACCGTGATCTGAAAAGCGACAATA
TTCTGATCGAAGAAGTTGATCCGTATCCGCTGCTGGCGATCACCGACTTC
GGCGAATGCCTGGATGACGCAGCTCTGAAACTGGTTGTCCCGTACATTAG
CAACTGTATCGATCGCGGCGGTAATAGTTCCCTGATGGCACCGGAAATTA
AAAACGCTGAACCGGGTCATAATTCTGTTCTGGATTATCGTCGCAGTGAC
GTCTGGGCGATTGGCTGCCTGGCCTTTGAAATCTTCGGTCAGGAAAACCC
GTTTCTGAATACCTTCGATTCATCGAGCTATCAAGAATACGAACTGCCGG
AACTGCCGTCTCGTGTCCCGCTGATTGTGCGTAAAATCGTTCGCAACCTG
CTGCGTAAAGATTCTAGTAATCGTCTGCCGGCATCCCTGGCTATTGCAGC
ACTGTCATTTTGGCGTTGGGGTCCGATCAATTGGCGTGCACTGATGATGT
ATGAAACCGGTCTGAGTAACCTGAATGAAGAAGATATTCAGCGTTGGCTG
TCGGAAACGGCCTTCCAGCTGAGCATTCAAATCATGCAGTGTCAAGCGCA
TGGTCACAAACTGACCCCGGAACTGCAGGCCAAATACCATTTTCTGGTCT
CAACGAGTGCCGCTGACATCAAACTGGCTGTGGAATCCCTGTATTGCTAA
GCGGCCGC
Amino Acid Sequence
View Sequence
MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTH
LKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGSDTTNSYKVGPRLGSRSCCSAVYATKCSCGKEYAMKMMFNYGNSKD
DFIRKNFLREYCLLSPLPVTRVDEMESSKNSQSRILVHPNIIRLDKVIID
EIQYCDDAMHEYPDALPPSPLNPSGLGRHRTMFLFMKKYDMTLREYVHSK
GIIPNDEATMLLLQLLQGVDYLNKNYIAHRDLKSDNILIEEVDPYPLLAI
TDFGECLDDAALKLVVPYISNCIDRGGNSSLMAPEIKNAEPGHNSVLDYR
RSDVWAIGCLAFEIFGQENPFLNTFDSSSYQEYELPELPSRVPLIVRKIV
RNLLRKDSSNRLPASLAIAALSFWRWGPINWRALMMYETGLSNLNEEDIQ
RWLSETAFQLSIQIMQCQAHGHKLTPELQAKYHFLVSTSAADIKLAVESL
YC*
LKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGSDTTNSYKVGPRLGSRSCCSAVYATKCSCGKEYAMKMMFNYGNSKD
DFIRKNFLREYCLLSPLPVTRVDEMESSKNSQSRILVHPNIIRLDKVIID
EIQYCDDAMHEYPDALPPSPLNPSGLGRHRTMFLFMKKYDMTLREYVHSK
GIIPNDEATMLLLQLLQGVDYLNKNYIAHRDLKSDNILIEEVDPYPLLAI
TDFGECLDDAALKLVVPYISNCIDRGGNSSLMAPEIKNAEPGHNSVLDYR
RSDVWAIGCLAFEIFGQENPFLNTFDSSSYQEYELPELPSRVPLIVRKIV
RNLLRKDSSNRLPASLAIAALSFWRWGPINWRALMMYETGLSNLNEEDIQ
RWLSETAFQLSIQIMQCQAHGHKLTPELQAKYHFLVSTSAADIKLAVESL
YC*
Vector Type
Bacterial
Antibiotic
Amp
Comments
in pET vector
100042 R 1000 1001 1047 19007
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