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Plasmid
pCDNA5-FRT/TO-PINK1-FLAG T257E
Parent Plasmid
pcDNA5 FRT/TO
Expressed
PINK1 FLAG T257E
DU Number
DU43714
Genbank
Q9BXM7
Tag
FLAG
Sequence of Insert
View Sequence
GGATCCGCCACCATGGCGGTGCGACAGGCGCTGGGCCGCGGCCTGCAGCT
GGGTCGAGCGCTGCTGCTGCGCTTCACGGGCAAGCCCGGCCGGGCCTACG
GCTTGGGGCGGCCGGGCCCGGCGGCGGGCTGTGTCCGCGGGGAGCGTCCA
GGCTGGGCCGCAGGACCGGGCGCGGAGCCTCGCAGGGTCGGGCTCGGGCT
CCCTAACCGTCTCCGCTTCTTCCGCCAGTCGGTGGCCGGGCTGGCGGCGC
GGTTGCAGCGGCAGTTCGTGGTGCGGGCCTGGGGCTGCGCGGGCCCTTGC
GGCCGGGCAGTCTTTCTGGCCTTCGGGCTAGGGCTGGGCCTCATCGAGGA
AAAACAGGCGGAGAGCCGGCGGGCGGTCTCGGCCTGTCAGGAGATCCAGG
CAATTTTTACCCAGAAAAGCAAGCCGGGGCCTGACCCGTTGGACACGAGA
CGCTTGCAGGGCTTTCGGCTGGAGGAGTATCTGATAGGGCAGTCCATTGG
TAAGGGCTGCAGTGCTGCTGTGTATGAAGCCACCATGCCTACATTGCCCC
AGAACCTGGAGGTGACAAAGAGCACCGGGTTGCTTCCAGGGAGAGGCCCA
GGTACCAGTGCACCAGGAGAAGGGCAGGAGCGAGCTCCGGGGGCCCCTGC
CTTCCCCTTGGCCATCAAGATGATGTGGAACATCTCGGCAGGTTCCTCCA
GCGAAGCCATCTTGAACACAATGAGCCAGGAGCTGGTCCCAGCGAGCCGA
GTGGCCTTGGCTGGGGAGTATGGAGCAGTCGAATACAGAAAATCCAAGAG
AGGTCCCAAGCAACTAGCCCCTCACCCCAACATCATCCGGGTTCTCCGCG
CCTTCACCTCTTCCGTGCCGCTGCTGCCAGGGGCCCTGGTCGACTACCCT
GATGTGCTGCCCTCACGCCTCCACCCTGAAGGCCTGGGCCATGGCCGGAC
GCTGTTCCTCGTTATGAAGAACTATCCCTGTACCCTGCGCCAGTACCTTT
GTGTGAACACACCCAGCCCCCGCCTCGCCGCCATGATGCTGCTGCAGCTG
CTGGAAGGCGTGGACCATCTGGTTCAACAGGGCATCGCGCACAGAGACCT
GAAATCCGACAACATCCTTGTGGAGCTGGACCCAGACGGCTGCCCCTGGC
TGGTGATCGCAGATTTTGGCTGCTGCCTGGCTGATGAGAGCATCGGCCTG
CAGTTGCCCTTCAGCAGCTGGTACGTGGATCGGGGCGGAAACGGCTGTCT
GATGGCCCCAGAGGTGTCCACGGCCCGTCCTGGCCCCAGGGCAGTGATTG
ACTACAGCAAGGCTGATGCCTGGGCAGTGGGAGCCATCGCCTATGAAATC
TTCGGGCTTGTCAATCCCTTCTACGGCCAGGGCAAGGCCCACCTTGAAAG
CCGCAGCTACCAAGAGGCTCAGCTACCTGCACTGCCCGAGTCAGTGCCTC
CAGACGTGAGACAGTTGGTGAGGGCACTGCTCCAGCGAGAGGCCAGCAAG
AGACCATCTGCCCGAGTAGCCGCAAATGTGCTTCATCTAAGCCTCTGGGG
TGAACATATTCTAGCCCTGAAGAATCTGAAGTTAGACAAGATGGTTGGCT
GGCTCCTCCAACAATCGGCCGCCACTTTGTTGGCCAACAGGCTCACAGAG
AAGTGTTGTGTGGAAACAAAAATGAAGATGCTCTTTCTGGCTAACCTGGA
GTGTGAAACGCTCTGCCAGGCAGCCCTCCTCCTCTGCTCATGGAGGGCAG
CCCTGGACTACAAGGACGACGATGACAAGTAGCGGCCGC
GGGTCGAGCGCTGCTGCTGCGCTTCACGGGCAAGCCCGGCCGGGCCTACG
GCTTGGGGCGGCCGGGCCCGGCGGCGGGCTGTGTCCGCGGGGAGCGTCCA
GGCTGGGCCGCAGGACCGGGCGCGGAGCCTCGCAGGGTCGGGCTCGGGCT
CCCTAACCGTCTCCGCTTCTTCCGCCAGTCGGTGGCCGGGCTGGCGGCGC
GGTTGCAGCGGCAGTTCGTGGTGCGGGCCTGGGGCTGCGCGGGCCCTTGC
GGCCGGGCAGTCTTTCTGGCCTTCGGGCTAGGGCTGGGCCTCATCGAGGA
AAAACAGGCGGAGAGCCGGCGGGCGGTCTCGGCCTGTCAGGAGATCCAGG
CAATTTTTACCCAGAAAAGCAAGCCGGGGCCTGACCCGTTGGACACGAGA
CGCTTGCAGGGCTTTCGGCTGGAGGAGTATCTGATAGGGCAGTCCATTGG
TAAGGGCTGCAGTGCTGCTGTGTATGAAGCCACCATGCCTACATTGCCCC
AGAACCTGGAGGTGACAAAGAGCACCGGGTTGCTTCCAGGGAGAGGCCCA
GGTACCAGTGCACCAGGAGAAGGGCAGGAGCGAGCTCCGGGGGCCCCTGC
CTTCCCCTTGGCCATCAAGATGATGTGGAACATCTCGGCAGGTTCCTCCA
GCGAAGCCATCTTGAACACAATGAGCCAGGAGCTGGTCCCAGCGAGCCGA
GTGGCCTTGGCTGGGGAGTATGGAGCAGTCGAATACAGAAAATCCAAGAG
AGGTCCCAAGCAACTAGCCCCTCACCCCAACATCATCCGGGTTCTCCGCG
CCTTCACCTCTTCCGTGCCGCTGCTGCCAGGGGCCCTGGTCGACTACCCT
GATGTGCTGCCCTCACGCCTCCACCCTGAAGGCCTGGGCCATGGCCGGAC
GCTGTTCCTCGTTATGAAGAACTATCCCTGTACCCTGCGCCAGTACCTTT
GTGTGAACACACCCAGCCCCCGCCTCGCCGCCATGATGCTGCTGCAGCTG
CTGGAAGGCGTGGACCATCTGGTTCAACAGGGCATCGCGCACAGAGACCT
GAAATCCGACAACATCCTTGTGGAGCTGGACCCAGACGGCTGCCCCTGGC
TGGTGATCGCAGATTTTGGCTGCTGCCTGGCTGATGAGAGCATCGGCCTG
CAGTTGCCCTTCAGCAGCTGGTACGTGGATCGGGGCGGAAACGGCTGTCT
GATGGCCCCAGAGGTGTCCACGGCCCGTCCTGGCCCCAGGGCAGTGATTG
ACTACAGCAAGGCTGATGCCTGGGCAGTGGGAGCCATCGCCTATGAAATC
TTCGGGCTTGTCAATCCCTTCTACGGCCAGGGCAAGGCCCACCTTGAAAG
CCGCAGCTACCAAGAGGCTCAGCTACCTGCACTGCCCGAGTCAGTGCCTC
CAGACGTGAGACAGTTGGTGAGGGCACTGCTCCAGCGAGAGGCCAGCAAG
AGACCATCTGCCCGAGTAGCCGCAAATGTGCTTCATCTAAGCCTCTGGGG
TGAACATATTCTAGCCCTGAAGAATCTGAAGTTAGACAAGATGGTTGGCT
GGCTCCTCCAACAATCGGCCGCCACTTTGTTGGCCAACAGGCTCACAGAG
AAGTGTTGTGTGGAAACAAAAATGAAGATGCTCTTTCTGGCTAACCTGGA
GTGTGAAACGCTCTGCCAGGCAGCCCTCCTCCTCTGCTCATGGAGGGCAG
CCCTGGACTACAAGGACGACGATGACAAGTAGCGGCCGC
Amino Acid Sequence
View Sequence
MAVRQALGRGLQLGRALLLRFTGKPGRAYGLGRPGPAAGCVRGERPGWAA
GPGAEPRRVGLGLPNRLRFFRQSVAGLAARLQRQFVVRAWGCAGPCGRAV
FLAFGLGLGLIEEKQAESRRAVSACQEIQAIFTQKSKPGPDPLDTRRLQG
FRLEEYLIGQSIGKGCSAAVYEATMPTLPQNLEVTKSTGLLPGRGPGTSA
PGEGQERAPGAPAFPLAIKMMWNISAGSSSEAILNTMSQELVPASRVALA
GEYGAVEYRKSKRGPKQLAPHPNIIRVLRAFTSSVPLLPGALVDYPDVLP
SRLHPEGLGHGRTLFLVMKNYPCTLRQYLCVNTPSPRLAAMMLLQLLEGV
DHLVQQGIAHRDLKSDNILVELDPDGCPWLVIADFGCCLADESIGLQLPF
SSWYVDRGGNGCLMAPEVSTARPGPRAVIDYSKADAWAVGAIAYEIFGLV
NPFYGQGKAHLESRSYQEAQLPALPESVPPDVRQLVRALLQREASKRPSA
RVAANVLHLSLWGEHILALKNLKLDKMVGWLLQQSAATLLANRLTEKCCV
ETKMKMLFLANLECETLCQAALLLCSWRAALDYKDDDDK*
GPGAEPRRVGLGLPNRLRFFRQSVAGLAARLQRQFVVRAWGCAGPCGRAV
FLAFGLGLGLIEEKQAESRRAVSACQEIQAIFTQKSKPGPDPLDTRRLQG
FRLEEYLIGQSIGKGCSAAVYEATMPTLPQNLEVTKSTGLLPGRGPGTSA
PGEGQERAPGAPAFPLAIKMMWNISAGSSSEAILNTMSQELVPASRVALA
GEYGAVEYRKSKRGPKQLAPHPNIIRVLRAFTSSVPLLPGALVDYPDVLP
SRLHPEGLGHGRTLFLVMKNYPCTLRQYLCVNTPSPRLAAMMLLQLLEGV
DHLVQQGIAHRDLKSDNILVELDPDGCPWLVIADFGCCLADESIGLQLPF
SSWYVDRGGNGCLMAPEVSTARPGPRAVIDYSKADAWAVGAIAYEIFGLV
NPFYGQGKAHLESRSYQEAQLPALPESVPPDVRQLVRALLQREASKRPSA
RVAANVLHLSLWGEHILALKNLKLDKMVGWLLQQSAATLLANRLTEKCCV
ETKMKMLFLANLECETLCQAALLLCSWRAALDYKDDDDK*
Vector Type
Mammalian
Antibiotic
Amp
Comments
in pcDNA5frtTO vector. Mark can you check we haven't previously made this - I can't find the clone on the database.
100042 R 1000 1001 1047 19007
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