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Plasmid
pET6HIS SUMO Pink1 tribolium 152-570 D359A
Parent Plasmid
pET15b 6His SUMO1
Expressed
6HIS SUMO Pink1 tribolium 152-570 D359A
DU Number
DU43669
Genbank
XP_968367
Tag
6His
SUMO
Species
Tribolium castaneum
Sequence of Insert
View Sequence
TTTGAATCTAACCCGATCACCCTGAATGATCTGAGTCTGGGTAAACCGAT
CGCCAAAGGCACGAACGGTGTGGTTTACTCCGCAAAAGTCAAAGATGACG
AAACCGATGACAATAAATATCCGTTTGCCCTGAAAATGATGTTCAACTAC
GATATTCAGAGCAATTCTATGGAAATCCTGAAAGCTATGTATCGTGAAAC
CGTTCCGGCGCGCATGTATTACTCAAACCACGACCTGAACAATTGGGAAA
TTGAACTGGCTAACCGTCGCAAACATCTGCCGCCGCACCCGAATATTGTG
GCAATCTTTAGCGTTTTCACCGATCTGATCCAAGAACTGGAAGGTTCTAA
AGACCTGTATCCGGCAGCGCTGCCGCCGCGTCTGCATCCGGAAGGTGAAG
GTCGCAACATGAGCCTGTTTCTGCTGATGAAACGTTATGATTGTAATCTG
CAGTCATTCCTGAGCACCGCTCCGAGTACCCGCACCTCCCTGCTGCTGCT
GGCCCAACTGCTGGAAGGCGTGGCGCATATGACGGCCCACGGTATTGCAC
ATCGTGATCTGAAATCAGACAACCTGCTGCTGGATACCTCAGAACCGGAA
TCGCCGATTCTGGTTATCTCGGcTTTTGGCTGCTGTCTGGCGGACAAAAC
CAACGGTCTGAGCCTGCCGTATACGTCTTACGAAATGGATAAAGGCGGTA
ATACGGCGCTGATGGCCCCGGAAATTATCTGCCAGAAACCGGGCACCTTT
AGTGTCCTGAATTATTCCAAAGCTGACCTGTGGGCGGTGGGTGCAATCGC
TTACGAAATCTTCAACTGTCACAATCCGTTCTACGGCCCGTCTCGCCTGA
AAAACTTCAACTACAAAGAAGGTGATCTGCCGAAACTGCCGGACGAAGTG
CCGACCGTTATTCAGGCACTGGTGGCTAACCTGCTGAAACGTAACCCGAA
TAAACGCCTGGACCCGGAAGTTGCGGCCAATGTCTGCCAGCTGTTTCTGT
GGGCACCGAGTACCTGGCTGAAACCGGGTCTGAAAGTTCCGACGTCAGGT
GAAATTCTGCAATGGCTGCTGTCGCTGACCACGAAAGTCCTGTGCGAAGG
CAAAATCAACAACAAATCCTTCGGTGAAAAATTCACCCGTAATTGGCGTC
GCACGTATCCGGAATACCTGCTGATTAGTTCCTTCCTGTGTCGCGCGAAA
CTGGCTAATGTTCGTAATGCTCTGCACTGGATTCAAGAAAACCTGCCGGA
ACTGGATTAAGCGGCCGC
CGCCAAAGGCACGAACGGTGTGGTTTACTCCGCAAAAGTCAAAGATGACG
AAACCGATGACAATAAATATCCGTTTGCCCTGAAAATGATGTTCAACTAC
GATATTCAGAGCAATTCTATGGAAATCCTGAAAGCTATGTATCGTGAAAC
CGTTCCGGCGCGCATGTATTACTCAAACCACGACCTGAACAATTGGGAAA
TTGAACTGGCTAACCGTCGCAAACATCTGCCGCCGCACCCGAATATTGTG
GCAATCTTTAGCGTTTTCACCGATCTGATCCAAGAACTGGAAGGTTCTAA
AGACCTGTATCCGGCAGCGCTGCCGCCGCGTCTGCATCCGGAAGGTGAAG
GTCGCAACATGAGCCTGTTTCTGCTGATGAAACGTTATGATTGTAATCTG
CAGTCATTCCTGAGCACCGCTCCGAGTACCCGCACCTCCCTGCTGCTGCT
GGCCCAACTGCTGGAAGGCGTGGCGCATATGACGGCCCACGGTATTGCAC
ATCGTGATCTGAAATCAGACAACCTGCTGCTGGATACCTCAGAACCGGAA
TCGCCGATTCTGGTTATCTCGGcTTTTGGCTGCTGTCTGGCGGACAAAAC
CAACGGTCTGAGCCTGCCGTATACGTCTTACGAAATGGATAAAGGCGGTA
ATACGGCGCTGATGGCCCCGGAAATTATCTGCCAGAAACCGGGCACCTTT
AGTGTCCTGAATTATTCCAAAGCTGACCTGTGGGCGGTGGGTGCAATCGC
TTACGAAATCTTCAACTGTCACAATCCGTTCTACGGCCCGTCTCGCCTGA
AAAACTTCAACTACAAAGAAGGTGATCTGCCGAAACTGCCGGACGAAGTG
CCGACCGTTATTCAGGCACTGGTGGCTAACCTGCTGAAACGTAACCCGAA
TAAACGCCTGGACCCGGAAGTTGCGGCCAATGTCTGCCAGCTGTTTCTGT
GGGCACCGAGTACCTGGCTGAAACCGGGTCTGAAAGTTCCGACGTCAGGT
GAAATTCTGCAATGGCTGCTGTCGCTGACCACGAAAGTCCTGTGCGAAGG
CAAAATCAACAACAAATCCTTCGGTGAAAAATTCACCCGTAATTGGCGTC
GCACGTATCCGGAATACCTGCTGATTAGTTCCTTCCTGTGTCGCGCGAAA
CTGGCTAATGTTCGTAATGCTCTGCACTGGATTCAAGAAAACCTGCCGGA
ACTGGATTAAGCGGCCGC
Amino Acid Sequence
View Sequence
MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTH
LKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGFESNPITLNDLSLGKPIAKGTNGVVYSAKVKDDETDDNKYPFALKM
MFNYDIQSNSMEILKAMYRETVPARMYYSNHDLNNWEIELANRRKHLPPH
PNIVAIFSVFTDLIQELEGSKDLYPAALPPRLHPEGEGRNMSLFLLMKRY
DCNLQSFLSTAPSTRTSLLLLAQLLEGVAHMTAHGIAHRDLKSDNLLLDT
SEPESPILVISAFGCCLADKTNGLSLPYTSYEMDKGGNTALMAPEIICQK
PGTFSVLNYSKADLWAVGAIAYEIFNCHNPFYGPSRLKNFNYKEGDLPKL
PDEVPTVIQALVANLLKRNPNKRLDPEVAANVCQLFLWAPSTWLKPGLKV
PTSGEILQWLLSLTTKVLCEGKINNKSFGEKFTRNWRRTYPEYLLISSFL
CRAKLANVRNALHWIQENLPELD*
LKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGFESNPITLNDLSLGKPIAKGTNGVVYSAKVKDDETDDNKYPFALKM
MFNYDIQSNSMEILKAMYRETVPARMYYSNHDLNNWEIELANRRKHLPPH
PNIVAIFSVFTDLIQELEGSKDLYPAALPPRLHPEGEGRNMSLFLLMKRY
DCNLQSFLSTAPSTRTSLLLLAQLLEGVAHMTAHGIAHRDLKSDNLLLDT
SEPESPILVISAFGCCLADKTNGLSLPYTSYEMDKGGNTALMAPEIICQK
PGTFSVLNYSKADLWAVGAIAYEIFNCHNPFYGPSRLKNFNYKEGDLPKL
PDEVPTVIQALVANLLKRNPNKRLDPEVAANVCQLFLWAPSTWLKPGLKV
PTSGEILQWLLSLTTKVLCEGKINNKSFGEKFTRNWRRTYPEYLLISSFL
CRAKLANVRNALHWIQENLPELD*
Vector Type
Bacterial
Antibiotic
Amp
Comments
in pET - please make wild-type and kinase-inactive versions (D359A)
100042 R 1000 1001 1047 19007
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