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Pink1 (Tribolium)

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Plasmid
pET6HIS SUMO Pink1 Tribolium 151-570 D359A
Parent Plasmid
pET15b 6His SUMO1
Expressed
6HIS SUMO Pink1 tribolium 151-570 D359A
DU Number
DU43667
Genbank
XP_968367
Tag
6His
SUMO
Species
Tribolium castaneum
Sequence of Insert
CGCTTTGAATCTAACCCGATCACCCTGAATGATCTGAGTCTGGGTAAACC
GATCGCCAAAGGCACGAACGGTGTGGTTTACTCCGCAAAAGTCAAAGATG
ACGAAACCGATGACAATAAATATCCGTTTGCCCTGAAAATGATGTTCAAC
TACGATATTCAGAGCAATTCTATGGAAATCCTGAAAGCTATGTATCGTGA
AACCGTTCCGGCGCGCATGTATTACTCAAACCACGACCTGAACAATTGGG
AAATTGAACTGGCTAACCGTCGCAAACATCTGCCGCCGCACCCGAATATT
GTGGCAATCTTTAGCGTTTTCACCGATCTGATCCAAGAACTGGAAGGTTC
TAAAGACCTGTATCCGGCAGCGCTGCCGCCGCGTCTGCATCCGGAAGGTG
AAGGTCGCAACATGAGCCTGTTTCTGCTGATGAAACGTTATGATTGTAAT
CTGCAGTCATTCCTGAGCACCGCTCCGAGTACCCGCACCTCCCTGCTGCT
GCTGGCCCAACTGCTGGAAGGCGTGGCGCATATGACGGCCCACGGTATTG
CACATCGTGATCTGAAATCAGACAACCTGCTGCTGGATACCTCAGAACCG
GAATCGCCGATTCTGGTTATCTCGGcTTTTGGCTGCTGTCTGGCGGACAA
AACCAACGGTCTGAGCCTGCCGTATACGTCTTACGAAATGGATAAAGGCG
GTAATACGGCGCTGATGGCCCCGGAAATTATCTGCCAGAAACCGGGCACC
TTTAGTGTCCTGAATTATTCCAAAGCTGACCTGTGGGCGGTGGGTGCAAT
CGCTTACGAAATCTTCAACTGTCACAATCCGTTCTACGGCCCGTCTCGCC
TGAAAAACTTCAACTACAAAGAAGGTGATCTGCCGAAACTGCCGGACGAA
GTGCCGACCGTTATTCAGGCACTGGTGGCTAACCTGCTGAAACGTAACCC
GAATAAACGCCTGGACCCGGAAGTTGCGGCCAATGTCTGCCAGCTGTTTC
TGTGGGCACCGAGTACCTGGCTGAAACCGGGTCTGAAAGTTCCGACGTCA
GGTGAAATTCTGCAATGGCTGCTGTCGCTGACCACGAAAGTCCTGTGCGA
AGGCAAAATCAACAACAAATCCTTCGGTGAAAAATTCACCCGTAATTGGC
GTCGCACGTATCCGGAATACCTGCTGATTAGTTCCTTCCTGTGTCGCGCG
AAACTGGCTAATGTTCGTAATGCTCTGCACTGGATTCAAGAAAACCTGCC
GGAACTGGATTAAGCGGCCGC

Amino Acid Sequence
MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTH
LKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGRFESNPITLNDLSLGKPIAKGTNGVVYSAKVKDDETDDNKYPFALK
MMFNYDIQSNSMEILKAMYRETVPARMYYSNHDLNNWEIELANRRKHLPP
HPNIVAIFSVFTDLIQELEGSKDLYPAALPPRLHPEGEGRNMSLFLLMKR
YDCNLQSFLSTAPSTRTSLLLLAQLLEGVAHMTAHGIAHRDLKSDNLLLD
TSEPESPILVISAFGCCLADKTNGLSLPYTSYEMDKGGNTALMAPEIICQ
KPGTFSVLNYSKADLWAVGAIAYEIFNCHNPFYGPSRLKNFNYKEGDLPK
LPDEVPTVIQALVANLLKRNPNKRLDPEVAANVCQLFLWAPSTWLKPGLK
VPTSGEILQWLLSLTTKVLCEGKINNKSFGEKFTRNWRRTYPEYLLISSF
LCRAKLANVRNALHWIQENLPELD*

Vector Type
Bacterial
Antibiotic
Amp
Comments
in pET - please make wild-type and kinase-inactive versions (D359A)
100042 R 1000 1001 1047 19007
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