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Plasmid
pET6HIS SUMO Pink1 Tribolium 146-570 D359A
Parent Plasmid
pET15b 6His SUMO1
Expressed
His-SUMO-TcPINK1 146-570 D359A
DU Number
DU43664
Genbank
XP_968367
Tag
6His
SUMO
Species
Tribolium castaneum
Sequence of Insert
View Sequence
GATATCGACGAAAGCCGCTTTGAATCTAACCCGATCACCCTGAATGATCT
GAGTCTGGGTAAACCGATCGCCAAAGGCACGAACGGTGTGGTTTACTCCG
CAAAAGTCAAAGATGACGAAACCGATGACAATAAATATCCGTTTGCCCTG
AAAATGATGTTCAACTACGATATTCAGAGCAATTCTATGGAAATCCTGAA
AGCTATGTATCGTGAAACCGTTCCGGCGCGCATGTATTACTCAAACCACG
ACCTGAACAATTGGGAAATTGAACTGGCTAACCGTCGCAAACATCTGCCG
CCGCACCCGAATATTGTGGCAATCTTTAGCGTTTTCACCGATCTGATCCA
AGAACTGGAAGGTTCTAAAGACCTGTATCCGGCAGCGCTGCCGCCGCGTC
TGCATCCGGAAGGTGAAGGTCGCAACATGAGCCTGTTTCTGCTGATGAAA
CGTTATGATTGTAATCTGCAGTCATTCCTGAGCACCGCTCCGAGTACCCG
CACCTCCCTGCTGCTGCTGGCCCAACTGCTGGAAGGCGTGGCGCATATGA
CGGCCCACGGTATTGCACATCGTGATCTGAAATCAGACAACCTGCTGCTG
GATACCTCAGAACCGGAATCGCCGATTCTGGTTATCTCGGcTTTTGGCTG
CTGTCTGGCGGACAAAACCAACGGTCTGAGCCTGCCGTATACGTCTTACG
AAATGGATAAAGGCGGTAATACGGCGCTGATGGCCCCGGAAATTATCTGC
CAGAAACCGGGCACCTTTAGTGTCCTGAATTATTCCAAAGCTGACCTGTG
GGCGGTGGGTGCAATCGCTTACGAAATCTTCAACTGTCACAATCCGTTCT
ACGGCCCGTCTCGCCTGAAAAACTTCAACTACAAAGAAGGTGATCTGCCG
AAACTGCCGGACGAAGTGCCGACCGTTATTCAGGCACTGGTGGCTAACCT
GCTGAAACGTAACCCGAATAAACGCCTGGACCCGGAAGTTGCGGCCAATG
TCTGCCAGCTGTTTCTGTGGGCACCGAGTACCTGGCTGAAACCGGGTCTG
AAAGTTCCGACGTCAGGTGAAATTCTGCAATGGCTGCTGTCGCTGACCAC
GAAAGTCCTGTGCGAAGGCAAAATCAACAACAAATCCTTCGGTGAAAAAT
TCACCCGTAATTGGCGTCGCACGTATCCGGAATACCTGCTGATTAGTTCC
TTCCTGTGTCGCGCGAAACTGGCTAATGTTCGTAATGCTCTGCACTGGAT
TCAAGAAAACCTGCCGGAACTGGATTAAGCGGCCGC
GAGTCTGGGTAAACCGATCGCCAAAGGCACGAACGGTGTGGTTTACTCCG
CAAAAGTCAAAGATGACGAAACCGATGACAATAAATATCCGTTTGCCCTG
AAAATGATGTTCAACTACGATATTCAGAGCAATTCTATGGAAATCCTGAA
AGCTATGTATCGTGAAACCGTTCCGGCGCGCATGTATTACTCAAACCACG
ACCTGAACAATTGGGAAATTGAACTGGCTAACCGTCGCAAACATCTGCCG
CCGCACCCGAATATTGTGGCAATCTTTAGCGTTTTCACCGATCTGATCCA
AGAACTGGAAGGTTCTAAAGACCTGTATCCGGCAGCGCTGCCGCCGCGTC
TGCATCCGGAAGGTGAAGGTCGCAACATGAGCCTGTTTCTGCTGATGAAA
CGTTATGATTGTAATCTGCAGTCATTCCTGAGCACCGCTCCGAGTACCCG
CACCTCCCTGCTGCTGCTGGCCCAACTGCTGGAAGGCGTGGCGCATATGA
CGGCCCACGGTATTGCACATCGTGATCTGAAATCAGACAACCTGCTGCTG
GATACCTCAGAACCGGAATCGCCGATTCTGGTTATCTCGGcTTTTGGCTG
CTGTCTGGCGGACAAAACCAACGGTCTGAGCCTGCCGTATACGTCTTACG
AAATGGATAAAGGCGGTAATACGGCGCTGATGGCCCCGGAAATTATCTGC
CAGAAACCGGGCACCTTTAGTGTCCTGAATTATTCCAAAGCTGACCTGTG
GGCGGTGGGTGCAATCGCTTACGAAATCTTCAACTGTCACAATCCGTTCT
ACGGCCCGTCTCGCCTGAAAAACTTCAACTACAAAGAAGGTGATCTGCCG
AAACTGCCGGACGAAGTGCCGACCGTTATTCAGGCACTGGTGGCTAACCT
GCTGAAACGTAACCCGAATAAACGCCTGGACCCGGAAGTTGCGGCCAATG
TCTGCCAGCTGTTTCTGTGGGCACCGAGTACCTGGCTGAAACCGGGTCTG
AAAGTTCCGACGTCAGGTGAAATTCTGCAATGGCTGCTGTCGCTGACCAC
GAAAGTCCTGTGCGAAGGCAAAATCAACAACAAATCCTTCGGTGAAAAAT
TCACCCGTAATTGGCGTCGCACGTATCCGGAATACCTGCTGATTAGTTCC
TTCCTGTGTCGCGCGAAACTGGCTAATGTTCGTAATGCTCTGCACTGGAT
TCAAGAAAACCTGCCGGAACTGGATTAAGCGGCCGC
Amino Acid Sequence
View Sequence
MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTH
LKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGDIDESRFESNPITLNDLSLGKPIAKGTNGVVYSAKVKDDETDDNKY
PFALKMMFNYDIQSNSMEILKAMYRETVPARMYYSNHDLNNWEIELANRR
KHLPPHPNIVAIFSVFTDLIQELEGSKDLYPAALPPRLHPEGEGRNMSLF
LLMKRYDCNLQSFLSTAPSTRTSLLLLAQLLEGVAHMTAHGIAHRDLKSD
NLLLDTSEPESPILVISAFGCCLADKTNGLSLPYTSYEMDKGGNTALMAP
EIICQKPGTFSVLNYSKADLWAVGAIAYEIFNCHNPFYGPSRLKNFNYKE
GDLPKLPDEVPTVIQALVANLLKRNPNKRLDPEVAANVCQLFLWAPSTWL
KPGLKVPTSGEILQWLLSLTTKVLCEGKINNKSFGEKFTRNWRRTYPEYL
LISSFLCRAKLANVRNALHWIQENLPELD*
LKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGDIDESRFESNPITLNDLSLGKPIAKGTNGVVYSAKVKDDETDDNKY
PFALKMMFNYDIQSNSMEILKAMYRETVPARMYYSNHDLNNWEIELANRR
KHLPPHPNIVAIFSVFTDLIQELEGSKDLYPAALPPRLHPEGEGRNMSLF
LLMKRYDCNLQSFLSTAPSTRTSLLLLAQLLEGVAHMTAHGIAHRDLKSD
NLLLDTSEPESPILVISAFGCCLADKTNGLSLPYTSYEMDKGGNTALMAP
EIICQKPGTFSVLNYSKADLWAVGAIAYEIFNCHNPFYGPSRLKNFNYKE
GDLPKLPDEVPTVIQALVANLLKRNPNKRLDPEVAANVCQLFLWAPSTWL
KPGLKVPTSGEILQWLLSLTTKVLCEGKINNKSFGEKFTRNWRRTYPEYL
LISSFLCRAKLANVRNALHWIQENLPELD*
Vector Type
Bacterial
Antibiotic
Amp
Comments
in pET - please make wild-type and kinase-inactive versions (D359A)
100042 R 1000 1001 1047 19007
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