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Plasmid
pET6HIS SUMO Pink1 Tribolium 151-570
Parent Plasmid
pET15b 6His SUMO1
Expressed
6HIS SUMO Pink1 tribolium 151-570
DU Number
DU43660
Genbank
XP_968367
Tag
6His
SUMO
Species
Tribolium castaneum
Sequence of Insert
View Sequence
CGCTTTGAATCTAACCCGATCACCCTGAATGATCTGAGTCTGGGTAAACC
GATCGCCAAAGGCACGAACGGTGTGGTTTACTCCGCAAAAGTCAAAGATG
ACGAAACCGATGACAATAAATATCCGTTTGCCCTGAAAATGATGTTCAAC
TACGATATTCAGAGCAATTCTATGGAAATCCTGAAAGCTATGTATCGTGA
AACCGTTCCGGCGCGCATGTATTACTCAAACCACGACCTGAACAATTGGG
AAATTGAACTGGCTAACCGTCGCAAACATCTGCCGCCGCACCCGAATATT
GTGGCAATCTTTAGCGTTTTCACCGATCTGATCCAAGAACTGGAAGGTTC
TAAAGACCTGTATCCGGCAGCGCTGCCGCCGCGTCTGCATCCGGAAGGTG
AAGGTCGCAACATGAGCCTGTTTCTGCTGATGAAACGTTATGATTGTAAT
CTGCAGTCATTCCTGAGCACCGCTCCGAGTACCCGCACCTCCCTGCTGCT
GCTGGCCCAACTGCTGGAAGGCGTGGCGCATATGACGGCCCACGGTATTG
CACATCGTGATCTGAAATCAGACAACCTGCTGCTGGATACCTCAGAACCG
GAATCGCCGATTCTGGTTATCTCGGATTTTGGCTGCTGTCTGGCGGACAA
AACCAACGGTCTGAGCCTGCCGTATACGTCTTACGAAATGGATAAAGGCG
GTAATACGGCGCTGATGGCCCCGGAAATTATCTGCCAGAAACCGGGCACC
TTTAGTGTCCTGAATTATTCCAAAGCTGACCTGTGGGCGGTGGGTGCAAT
CGCTTACGAAATCTTCAACTGTCACAATCCGTTCTACGGCCCGTCTCGCC
TGAAAAACTTCAACTACAAAGAAGGTGATCTGCCGAAACTGCCGGACGAA
GTGCCGACCGTTATTCAGGCACTGGTGGCTAACCTGCTGAAACGTAACCC
GAATAAACGCCTGGACCCGGAAGTTGCGGCCAATGTCTGCCAGCTGTTTC
TGTGGGCACCGAGTACCTGGCTGAAACCGGGTCTGAAAGTTCCGACGTCA
GGTGAAATTCTGCAATGGCTGCTGTCGCTGACCACGAAAGTCCTGTGCGA
AGGCAAAATCAACAACAAATCCTTCGGTGAAAAATTCACCCGTAATTGGC
GTCGCACGTATCCGGAATACCTGCTGATTAGTTCCTTCCTGTGTCGCGCG
AAACTGGCTAATGTTCGTAATGCTCTGCACTGGATTCAAGAAAACCTGCC
GGAACTGGATTAAGCGGCCGC
GATCGCCAAAGGCACGAACGGTGTGGTTTACTCCGCAAAAGTCAAAGATG
ACGAAACCGATGACAATAAATATCCGTTTGCCCTGAAAATGATGTTCAAC
TACGATATTCAGAGCAATTCTATGGAAATCCTGAAAGCTATGTATCGTGA
AACCGTTCCGGCGCGCATGTATTACTCAAACCACGACCTGAACAATTGGG
AAATTGAACTGGCTAACCGTCGCAAACATCTGCCGCCGCACCCGAATATT
GTGGCAATCTTTAGCGTTTTCACCGATCTGATCCAAGAACTGGAAGGTTC
TAAAGACCTGTATCCGGCAGCGCTGCCGCCGCGTCTGCATCCGGAAGGTG
AAGGTCGCAACATGAGCCTGTTTCTGCTGATGAAACGTTATGATTGTAAT
CTGCAGTCATTCCTGAGCACCGCTCCGAGTACCCGCACCTCCCTGCTGCT
GCTGGCCCAACTGCTGGAAGGCGTGGCGCATATGACGGCCCACGGTATTG
CACATCGTGATCTGAAATCAGACAACCTGCTGCTGGATACCTCAGAACCG
GAATCGCCGATTCTGGTTATCTCGGATTTTGGCTGCTGTCTGGCGGACAA
AACCAACGGTCTGAGCCTGCCGTATACGTCTTACGAAATGGATAAAGGCG
GTAATACGGCGCTGATGGCCCCGGAAATTATCTGCCAGAAACCGGGCACC
TTTAGTGTCCTGAATTATTCCAAAGCTGACCTGTGGGCGGTGGGTGCAAT
CGCTTACGAAATCTTCAACTGTCACAATCCGTTCTACGGCCCGTCTCGCC
TGAAAAACTTCAACTACAAAGAAGGTGATCTGCCGAAACTGCCGGACGAA
GTGCCGACCGTTATTCAGGCACTGGTGGCTAACCTGCTGAAACGTAACCC
GAATAAACGCCTGGACCCGGAAGTTGCGGCCAATGTCTGCCAGCTGTTTC
TGTGGGCACCGAGTACCTGGCTGAAACCGGGTCTGAAAGTTCCGACGTCA
GGTGAAATTCTGCAATGGCTGCTGTCGCTGACCACGAAAGTCCTGTGCGA
AGGCAAAATCAACAACAAATCCTTCGGTGAAAAATTCACCCGTAATTGGC
GTCGCACGTATCCGGAATACCTGCTGATTAGTTCCTTCCTGTGTCGCGCG
AAACTGGCTAATGTTCGTAATGCTCTGCACTGGATTCAAGAAAACCTGCC
GGAACTGGATTAAGCGGCCGC
Amino Acid Sequence
View Sequence
MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTH
LKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGRFESNPITLNDLSLGKPIAKGTNGVVYSAKVKDDETDDNKYPFALK
MMFNYDIQSNSMEILKAMYRETVPARMYYSNHDLNNWEIELANRRKHLPP
HPNIVAIFSVFTDLIQELEGSKDLYPAALPPRLHPEGEGRNMSLFLLMKR
YDCNLQSFLSTAPSTRTSLLLLAQLLEGVAHMTAHGIAHRDLKSDNLLLD
TSEPESPILVISDFGCCLADKTNGLSLPYTSYEMDKGGNTALMAPEIICQ
KPGTFSVLNYSKADLWAVGAIAYEIFNCHNPFYGPSRLKNFNYKEGDLPK
LPDEVPTVIQALVANLLKRNPNKRLDPEVAANVCQLFLWAPSTWLKPGLK
VPTSGEILQWLLSLTTKVLCEGKINNKSFGEKFTRNWRRTYPEYLLISSF
LCRAKLANVRNALHWIQENLPELD*
LKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGRFESNPITLNDLSLGKPIAKGTNGVVYSAKVKDDETDDNKYPFALK
MMFNYDIQSNSMEILKAMYRETVPARMYYSNHDLNNWEIELANRRKHLPP
HPNIVAIFSVFTDLIQELEGSKDLYPAALPPRLHPEGEGRNMSLFLLMKR
YDCNLQSFLSTAPSTRTSLLLLAQLLEGVAHMTAHGIAHRDLKSDNLLLD
TSEPESPILVISDFGCCLADKTNGLSLPYTSYEMDKGGNTALMAPEIICQ
KPGTFSVLNYSKADLWAVGAIAYEIFNCHNPFYGPSRLKNFNYKEGDLPK
LPDEVPTVIQALVANLLKRNPNKRLDPEVAANVCQLFLWAPSTWLKPGLK
VPTSGEILQWLLSLTTKVLCEGKINNKSFGEKFTRNWRRTYPEYLLISSF
LCRAKLANVRNALHWIQENLPELD*
Vector Type
Bacterial
Antibiotic
Amp
Comments
in pET - please make wild-type and kinase-inactive versions (D359A)
100042 R 1000 1001 1047 19007
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